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1. Improvement of ethanol production from D-lactic acid by constitutive expression of lactate transporter Jen1p in Saccharomyces cerevisiae.

Author

Wakamatsu M, Tomitaka M, Tani T, Taguchi H, Kida K, and Akamatsu T

Subjects
Gene Expression, Ethanol metabolism, Genetic Engineering methods, Lactic Acid metabolism, Monocarboxylic Acid Transporters genetics, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Symporters genetics
Abstract

To improve ethanol production from D-lactate, Jen1p, a monocarboxylate-proton symporter, was constitutively expressed in Saccharomyces cerevisiae NAM34-4C. The mutant produced 2.4 g/L of ethanol, approximately 2.4 times higher than that of the wild-type strain. A monocarboxylate/proton symporter gene (JEN1) null mutant was also constructed. It produced 0.19 g/L of ethanol, 5 times lower than that of the wild-type strain.

Published
2013
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