1. Correlation of Interleukin-2 Receptor Expression With Tissue-Specific Growth of an Interleukin-3-Dependent Autocrine Leukemia
- Author
-
Ian G. Young, H. D. Campbell, Terry Robins, Andrew J. Hapel, and Rhodri Ceredig
- Subjects
Interleukin 2 ,Cancer Research ,Receptor expression ,Genetic Vectors ,Leukemia inhibitory factor receptor ,Biology ,Transfection ,Cell Line ,Mice ,medicine ,Animals ,Lymphocytes ,Receptors, Immunologic ,Autocrine signalling ,Interleukin 3 ,Leukemia, Experimental ,Interleukin ,Receptors, Interleukin-2 ,Flow Cytometry ,medicine.disease ,Virology ,Molecular biology ,Clone Cells ,Leukemia ,Retroviridae ,Genes ,Oncology ,Leukemia, Myeloid ,Cell culture ,Interleukin-2 ,Interleukin-3 ,medicine.drug - Abstract
An autocrine leukemia (FDC-P1-IL3) has been developed using a retroviral vector containing the interleukin-3 (IL-3) gene to transfect the IL-3-dependent cell line FDC-P1. When leukemia cells were reisolated from experimental animals, it was found that levels of interleukin-2 (IL-2) receptor (IL-2R) expression were greater on cells isolated from the lymph node than on cells isolated from the spleen. Cloned sublines of FDC-P1-IL3 were selected by flow microfluorometry for high or low levels of IL-2R expression. Those clones that expressed high levels of IL-2R grew preferentially in the lymph node. Although IL-2 is not mitogenic for FDC-P1 cells and does not increase the rate of growth of FDC-P1-IL3 cells in vitro, the cloning efficiency of FDC-P1-IL3 is increased fourfold in the presence of IL-2. These observations suggest that the IL-2R on FDC-P1-IL3 cells plays an important role in modulating the growth of this leukemia in sites that contain high levels of IL-2.
- Published
- 1988
- Full Text
- View/download PDF