1. Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
- Author
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Tomas Cermak, Clarice Schmidt, Daniel F. Voytas, Erin L. Doyle, Aaron W. Hummel, Michelle Christian, Adam J. Bogdanove, and Feng Zhang
- Subjects
Transcriptional Activation ,Transcription Activator-Like Effectors ,Xanthomonas ,DNA Repair ,Transcription, Genetic ,Recombinant Fusion Proteins ,Biology ,Xanthomonas campestris ,Genome engineering ,chemistry.chemical_compound ,Genome editing ,TAL effector ,Notes ,Catalytic Domain ,Genetics ,DNA Breaks, Double-Stranded ,Protein–DNA interaction ,Deoxyribonucleases, Type II Site-Specific ,Transcription activator-like effector nuclease ,Binding Sites ,Zinc Fingers ,DNA ,Zinc finger nuclease ,DNA-Binding Proteins ,chemistry ,Gene Targeting ,Genetic Engineering ,Transcription Factors - Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
- Published
- 2010
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