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Introduction The riverine population resident at banks of Amazon river (Amapá, Brazil) has particularities related to health-disease process and socioepidemiologic demands, that represents a challenge for appointment of professionals in the unique health system. The project Mandic Borderless has a purpose to attend, in a complete and humanized way, populations without assistance in places of difficult access. Objectives Describe the experience and results obtained from the health care to riverine population in the region along Amazon River, Amapá, Brazil, attended during an expedition in 2020. Descriptive cross-sectional survey. The health-care staff that performed the consultations was composed of doctor, dentists, nurses and medical students, who work in a college located in the interior of the state of São Paulo. Results About 4,500 riverine populations were attended indirectly and other 1,775 directly by many medical specialties, such as: clinical medicine, gynecology, pediatrics, ophthalmology, dermatology, ultrasonography, nursery and odontology work for free. And also collective health education actions were carried out, addressing topics such as: oral health, scabies, pediculosis (lice), first aid, hypertension, diabetes, sexually transmitted diseases prevention and contraceptive methods. More than 300 patients received toothpaste kits and were instructed about oral health. Conclusions The expedition collected data about isolate population showing their necessary and provide an innovated experience in the medical teachings, considering the knowledge about the disease health process and the integrated and humanity care. Key messages Provide for medical students a vision about communities who lives in a geographic isolation. There are necessary of an upgrade in access a basic e essential services for the riverine population.