1. TheMedicago truncatulaE3 Ubiquitin Ligase PUB1 Interacts with the LYK3 Symbiotic Receptor and Negatively Regulates Infection and Nodulation
- Author
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Susana Rivas, Benoit Lefebvre, Ton Timmers, Dörte Klaus-Heisen, Laurent Deslandes, Christine Hervé, Malick Mbengue, Fernanda de Carvalho-Niebel, Julie V. Cullimore, Solène Froidure, Sylvie Camut, Sandra Moreau, Unité mixte de recherche interactions plantes-microorganismes, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), European Community funded Research Training Network 'NODPERCEPTION', French Ministry for Higher Education and Research, and ANR-05-BLAN-0243,NodBindsLysM,Etude multidisciplinaire des Interactions signal Nod / LysM récepteurs kinases dans la symbiose Rhizobia-Légumineuses(2005)
- Subjects
0106 biological sciences ,symbiotic receptor ,Plant Science ,luzerne ,Plant Root Nodulation ,01 natural sciences ,nitrogen ,Nod factor ,Ubiquitin ,Gene Expression Regulation, Plant ,medicago trunculata ,Génétique des plantes ,sinorhizobium meliloti ,nodulation ,Research Articles ,Plant Proteins ,azote ,0303 health sciences ,Sinorhizobium meliloti ,fourrage ,biology ,Kinase ,ubiquitin ligase ,fabaceae ,food and beverages ,[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics ,Plants, Genetically Modified ,Medicago truncatula ,Ubiquitin ligase ,Cell biology ,Biochemistry ,RNA Interference ,Signal transduction ,symbiose ,récepteur ,Signal Transduction ,Ubiquitin-Protein Ligases ,Molecular Sequence Data ,Plants genetics ,légumineuse ,[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics ,03 medical and health sciences ,Two-Hybrid System Techniques ,Tobacco ,Symbiosis ,030304 developmental biology ,fungi ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Cell Biology ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,infection ,[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding ,Protein kinase domain ,biology.protein ,bacteria ,010606 plant biology & botany - Abstract
L'article original est publié par The American Society of Plant Biologists; International audience; LYK3 is a lysin motif receptor-like kinase of Medicago truncatula, which is essential for the establishment of the nitrogenfixing, root nodule symbiosis with Sinorhizobium meliloti. LYK3 is a putative receptor of S. meliloti Nod factor signals, but little is known of how it is regulated and how it transduces these symbiotic signals. In a screen for LYK3-interacting proteins, we identified M. truncatula Plant U-box protein 1 (PUB1) as an interactor of the kinase domain. In planta, both proteins are localized and interact in the plasma membrane. In M. truncatula, PUB1 is expressed specifically in symbiotic conditions, is induced by Nod factors, and shows an overlapping expression pattern with LYK3 during nodulation. Biochemical studies show that PUB1 has a U-box–dependent E3 ubiquitin ligase activity and is phosphorylated by the LYK3 kinase domain. Overexpression and RNA interference studies in M. truncatula show that PUB1 is a negative regulator of the LYK3 signaling pathway leading to infection and nodulation and is important for the discrimination of rhizobia strains producing variant Nod factors. The potential role of PUB E3 ubiquitin ligases in controlling plant–microbe interactions and development through interacting with receptor-like kinases is discussed.
- Published
- 2010
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