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Start Over Author "Taiga Hara" Publisher oxford university press (oup)
3 results on '"Taiga Hara"'

1. Low-density lipoprotein apheresis for haemodialysis patients with peripheral arterial disease reduces reactive oxygen species production via suppression of NADPH oxidase gene expression in leucocytes

Author

Chikako Higashiyama, Masakazu Kohno, Hideyasu Kiyomoto, Kumiko Moriwaki, Kumiko Kaifu, Hirofumi Hitomi, Akira Nishiyama, Genei Ihara, Yoshiko Fujita, and Taiga Hara

Subjects
Male, medicine.medical_specialty, medicine.disease_cause, Internal medicine, Leukocytes, medicine, TBARS, Humans, Aged, Aged, 80 and over, Peripheral Vascular Diseases, Transplantation, NADPH oxidase, biology, Vascular disease, business.industry, C-reactive protein, NADPH Oxidases, Arteriosclerosis, Middle Aged, medicine.disease, Lipoproteins, LDL, Endocrinology, Nephrology, Immunology, Blood Component Removal, biology.protein, Female, lipids (amino acids, peptides, and proteins), P22phox, Reactive Oxygen Species, business, Oxidative stress, Lipoprotein
Abstract

Background Peripheral arterial disease (PAD) is a major complication of haemodialysis (HD), especially in patients with diabetes mellitus. Although previous reports have indicated that low-density lipoprotein apheresis (LDL-A) improves arteriosclerosis in PAD patients, the mechanism by which LDL-A affects PAD is still unclear. In this study, we tested the hypothesis that LDL-A attenuates reactive oxygen species (ROS) production in HD patients with PAD. Methods Twenty HD patients with PAD were investigated in this study. Clinical effects were evaluated by thermography and angiography. Oxidative stress in serum was evaluated by thiobarbituric acid reactive substances (TBARS) and expression of p22phox mRNA. Results Ischaemic symptoms due to PAD were gradually improved in 13 patients (65%) after LDL-A. One session of LDL-A removed approximately 75% of LDL from serum. Some patients exhibited dramatic improvement of severe symptoms of PAD such as skin ulcers after serial performance of LDL-A. The levels of LDL cholesterol, malondialdehyde-modified LDL, high-sensitivity C-reactive protein, vascular endothelial growth factor, international normalized ratio of pro-thrombin time and bradykinin were decreased after a single session of LDL-A, although there were no additional changes after 10 sessions of LDL-A. The levels of fibrinogen and p22phox mRNA were decreased by a single session of LDL-A, and these decreases continued over the entire period of treatment. TBARS was decreased after a course of LDL-A. Conclusions LDL-A improved ischaemic symptoms in HD patients with PAD by reducing ROS production in leucocytes. We conclude that LDL-A is an effective therapy for patients with HD complicated by PAD.

Published
2009
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2. Insulin attenuates apoptosis induced by high glucose via the PI3-kinase/Akt pathway in rat peritoneal mesothelial cells

Author

Hideyasu Kiyomoto, Yoshiko Fujita, Keisuke Matsubara, Masakazu Kohno, Hirofumi Hitomi, Noriko Sugasawa, Daisuke Nagata, Kumiko Moriwaki, Kumiko Kaifu, Taiga Hara, Akira Nishiyama, and Genei Ihara

Subjects
Male, medicine.medical_specialty, Morpholines, medicine.medical_treatment, Cell Culture Techniques, Apoptosis, Rats, Sprague-Dawley, Wortmannin, Phosphatidylinositol 3-Kinases, chemistry.chemical_compound, Internal medicine, medicine, Animals, Hypoglycemic Agents, Insulin, Phosphatidylinositol, Phosphorylation, Protein kinase B, PI3K/AKT/mTOR pathway, Phosphoinositide-3 Kinase Inhibitors, Transplantation, biology, Kinase, business.industry, Akt/PKB signaling pathway, Epithelial Cells, Rats, Androstadienes, Insulin receptor, Glucose, Endocrinology, chemistry, Chromones, Nephrology, biology.protein, Peritoneum, business, Proto-Oncogene Proteins c-akt, Signal Transduction
Abstract

Background Peritoneal mesothelial cells play an important role in peritoneal dialysis and are often exposed to dialysis fluid containing high glucose levels. Loss of peritoneal function is a major complication associated with long-term peritoneal dialysis. In this study, we hypothesized that high glucose levels induce apoptosis, and that insulin attenuates this apoptosis in peritoneal mesothelial cells. To clarify this hypothesis, we examined the effects of insulin on the phosphatidylinositol 3-kinase/Akt signaling pathway and apoptosis in rat peritoneal mesothelial cells. Methods Phosphorylated insulin receptor and Akt were detected by western blot analysis. Apoptosis was evaluated by measuring caspase 3 activity and by TUNNEL staining. Results Insulin (100 nmol/L) increased tyrosine phosphorylation of insulin receptor in peritoneal mesothelial cells. Furthermore, insulin (1-100 nmol/L) dose-dependently stimulated Akt phosphorylation. Treatment with the phosphatidylinositol 3-kinase inhibitors wortmannin (100 nmol/L) and LY294002 (10 micromol/L) attenuated insulin-induced Akt phosphorylation, indicating that insulin phosphorylates Akt via a phosphatidylinositol 3-kinase-dependent pathway. Insulin attenuated caspase 3 activity and decreased the number of TUNNEL-positive cells. The phosphatidylinositol 3-kinase inhibitors and overexpression of a dominant-negative mutant of Akt inhibited the effect of insulin on apoptosis. Conclusions The present data indicate that insulin attenuates high glucose-induced apoptosis via the phosphatidylinositol 3-kinase/Akt signaling pathway in peritoneal mesothelial cells. Therefore, the insulin signaling pathway may play a protective role in peritoneal function.

Published
2008
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3. Peritoneal dialysis - 3

Author

J. Rottembourg, Ea Wha Kang, Yilmaz Selcuk, Yong-Lim Kim, Declan de Freitas, Friedrich Prischl, Yoshiko Fujita, Javier Villacorta, Yiu Wing Ho, Ingrid Hofinger, Anna Casula, Yu Ting Tan, Jose C. Divino Filho, Watske Smit, Mustafa Comert, Miguel Perez Fontan, Juan Pérez-Martínez, Serhan Tuglular, Kyung-Woo Yoon, Ana Rodriguez-Carmona, Yoko Tanihata, Yasemin Soyoral, Vera M.S. Belangero, Alena Parikova, Kay B. Tan, Hye-Myung Ryu, José Luis Górriz Teruel, Annemarie Liegeois, Peter Rutherford, Annemieke M. Coester, Carmen A. Vlahu, Paul Taylor, Byung-Hee Lee, Adriana Loza, Yoko Nishijima, Yu Jin Jung, Terrance Wu, Hirofumi Hitomi, Keitaro Yokoyama, Murvet Yilmaz, Kyung Jin Lee, Selahattin Bicak, Hans Vink, Huseyin Toz, Sik Lee, Giovanni Cancarini, Woon Or Lam, Duk Hee Kang, Kumiko Kaifu, Carmen Gómez, Maurice Wan, Young-Hee Kim, Eyup Kulah, Luigi Manili, Hideki Nishimura, Lynne Curtis, Simon J. Davies, Hiroshi Hayakawa, Ayse Arar, Yoshitaka Maeda, W. Pronai, Elbis Ahbap, Giuseppe Mazzola, Ravi Pararajasingham, Ira Davis, Ya-fei Yang, Eveline Lee, Hyuk-Joon Choi, Roberto Pecoits-Filho, Raymond T. Krediet, Ho Yung Lee, Jose A. Meneses, Dae Suk Han, Lawrence P. McMahon, Ozge Timur, Yan Li, Natalia Fernandes, Joaquín Ortuño, Jun-Young Do, Stanley Fan, Tsutomu Sanaka, Maria C. Villegas, Sennur Kose Budak, Julia Klein, Bregtje A. Lemkes, Hugo Breien, Young Sook Lee, Haridian Sosa, Martin Zeier, Vedat Schwenger, Akihiko Matsuda, Friedo W. Dekker, Baris Doner, Jung Eun Lee, Yalcin Solak, Mourad Farouk, Antonio Alberto Lopes, Abdulkadir Unsal, Mina Yu, Kleyton A. Bastos, Lutfullah Altintepe, Jesus Montenegro, Andres Lopez Muñiz, Betul Erismis, Murat Can, Devrim Bozkurt, Takenori Funaki, Lars P. Kihm, Wieneke Michels, Renato T. Goncalves, Louese Dunn, Seung Hyun Lee, Marina Avramovic, Francesca Valerio, Kyu-Bok Choi, Chieko Higuchi, Tatsuo Hosoya, Paul Brenchley, Reinhard Kramar, Jong Won Park, Hideyasu Kiyomoto, Els Boeschoten, Jeung Eun Lee, Afshin Tavakoli, Cetin Ozener, Yoshindo Kawaguchi, Tadashi Sofue, Chan-Duck Kim, J. De Meester, Minoru Kubota, Sandra van Dijk, Gregory Ehrlich, Sandhya Seneviratne, Seung Hyeok Han, Burak Sayin, Juan Pérez, Anurag Singh, Kyung Pyo Kang, Ibrahim Guney, Kyu Hun Choi, Gulsah Sasak, Arafat Mirza, Arzu Kahveci, Yener Koc, Kyu-Hyang Cho, Dick G. Struijk, Siren Sezer, Zeynep Biyik, Hasan Kayabasi, Huang-Joe Wang, Huseyin Atalay, Jung-Ju Seo, Hidetomo Nakamoto, Angela Summers, Kumiko Moriwaki, Archna Sinha, Jin-Jhung Yeh, Tsun Gun Ng, Ahmet Dursun, Sang Choel Lee, Dong Ryeol Ryu, Elif Ari, Ken Izumori, Marija Andjelkovic, Anita Saxena, Marie-Luise Gross, Mehdi Yeksan, Pinar Cetin, Hiromichi Suzuki, Murat Biyik, Massimo Sandrini, David Ansell, Hee-Jeong Choi, Masaaki Tokuda, Zeki Tonbul, Sung Kwang Park, Bratislav Apostolovic, Satoshi Nishioka, Soo Young Yoon, Osamu Ikehara, Masakazu Kohno, Ae Sin Lee, In-Kyong Hur, Audrey M. Hutchcraft, Taiga Hara, John E. Sanderson, Nurhan Ozdemir, Amit Gupta, Ju-Min Yook, Kyun-Il Yoon, Zhangsuo Liu, Sang Yong Lee, Sung Kyew Kang, Yuk Yee Cheng, Seng Hoe Tan, Teresa Garcia Falcon, Huseyin Begenik, Biju John, Raziye Yazici, Dick Struijk, Soner Duman, Ian Bridges, Shin Wook Kang, Sun-Hee Park, Ji-Hyung Cho, Sara Jiménez, Valerio Vizzardi, Hakki Arikan, Guldeniz Karadeniz, Suleyman Turk, Jose Ramon Rodriguez-Palomares, Ana Marta Gomes, Tetsuya Mitarai, Jakob Arhem, Mehmet Naci Aldemir, A. Oguz Akgun, Manfred Wallner, Won Kim, Mari Ishida, Tae Hwan Lee, Sait Şen, Paul J. Thornalley, Mehmet Emin Kucukoglu, Rosalind Williams, F. Wenzelburger, Carmen Gómez-Roldán, M. Feriani, Eva Seiringer, Yudo Tanno, Bruno Remacle, Yoshihiro Obara, Marion Verduijn, Titus Augustine, Maite Rivera, Bo Ekelund, Erol Aktunç, Victor Burguera, Akira Nishiyama, Kitae Bang, Duk Hoon Kim, Synke van der Heyden, Sung-Bok Jung, Hiroyasu Yamamoto, Seung-Jung Kim, Erkan Oztekin, Ana Guitian, Helen Hurst, Raj Kumar Sharma, Ji-Young Choi, Kia Chong Chua, Seong Suk Kim, Narayan Prasad, Meng Eng Tay, Reha Erkoc, Sonja Zehetmayer, Sandra Mueller-Krebs, and Catherine De Vos

Subjects
Transplantation, medicine.medical_specialty, Nephrology, business.industry, medicine.medical_treatment, Urology, medicine, business, Peritoneal dialysis
Published
2009
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