1. Penicillin susceptibility among invasive MSSA infections: a multicentre study in 16 Spanish hospitals
- Author
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Mama, O.M., Aspiroz, C., Lozano, C., Ruiz-Ripa, L., Azcona, J.M., Seral, C., Cercenado, E., López-Cerero, L., Palacián, P., Belles-Belles, A., Berdonces, P., Siller, M., Aguirre-Quiñonero, A., Zarazaga, M., Torres, C, Castillo, J., Rezusta, A., Calleja, A.I., and on behalf of the Spanish Study Group of Clinical S. aureus CC398
- Subjects
Methicillin-Resistant Staphylococcus aureus ,0301 basic medicine ,Microbiology (medical) ,Staphylococcus aureus ,Fusidic acid ,030106 microbiology ,Erythromycin ,Mupirocin ,Microbial Sensitivity Tests ,Penicillins ,Biology ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Animals ,Pharmacology (medical) ,Typing ,Pharmacology ,Tetracycline Resistance ,Staphylococcal Infections ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,Hospitals ,Anti-Bacterial Agents ,Ciprofloxacin ,Penicillin ,030104 developmental biology ,Infectious Diseases ,chemistry ,Methicillin Susceptible Staphylococcus Aureus ,medicine.drug - Abstract
Objectives To determine the prevalence of penicillin susceptibility among MSSA causing bloodstream infections (BSIs) in 16 Spanish hospitals and to characterize the penicillin-susceptible MSSA (MSSA-PENS) isolates. Methods A total of 1011 Staphylococcus aureus isolates were collected from blood cultures in 16 Spanish hospitals during 2018–19 (6–12 months) and their susceptibility to 18 antimicrobials was determined. The MSSA-PENS isolates were selected and examined by PCR to determine the presence of the blaZ gene, other resistance genes and the genes lukF/lukS-PV, eta, etb and tst. The immune evasion cluster (IEC) type was also analysed. All the MSSA-PENS isolates were submitted to S. aureus protein A (spa) typing and the clonal complexes (CCs) were assigned according to their spa type. Results The prevalence of MSSA was 74.6% (754/1011) and 14.9% (151/1011) were MSSA-PENS-blaZnegative. MSSA-PENS-blaZnegative isolates (n = 151) were ascribed to 88 spa types and 11 CCs. The most frequent CCs were CC5 (35/151) and CC398 (25/151), with t002-CC5 and t571-CC398 being the most common lineages. Pan-susceptibility was identified in 117 of the 151 MSSA-PENS-blaZnegative isolates (77.5%). In the remaining isolates, erythromycin and clindamycin resistance was the most frequent resistance found, although tobramycin, ciprofloxacin, fusidic acid, mupirocin and/or tetracycline resistance was also detected. Thirty-eight MSSA-PENS-blaZnegative isolates were IEC negative and four isolates were Panton–Valentine leucocidin (‘PVL’) positive. Conclusions A high penicillin susceptibility rate was detected among MSSA, opening therapeutic opportunities for BSIs. The emergence of new successful MSSA-PENS clones could be responsible for these data. The detection among MSSA-PENS-blaZnegative isolates of the clonal lineage CC398 or the absence of an IEC raises questions about their possible animal origin, requiring further analysis.
- Published
- 2021
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