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The article focuses on the study regarding the winged bean chymotrypsin-trypsin inhibitor (WbCTI) cloning, characterization, and purification. The study isolated genomic DNA and RNA from winged bean and used Escherichia coli strain in the cloning experiments and for plasmid propagation. The study showed that WbCTI and Kunitz-type trypsin isolated from soybeans encoding and Delonix regia (DrTI) were intronless and recombinant protein (rWbCTI) binded the proteases in a 1:1 molar ratio.

The article presents a medical case of a diabetic man, who had poor compliance to treatment with left lower limb pain and deformity of his left foot for two months. On evaluation, he had bilateral loss of pain, touch, temperature, and vibration sense below the ankle joint. His vibration perception threshold was increased to 46 mV on the left side and 38 mV on the right side. The overall picture was of chronic neuroarthropathic joint due to diabetes mellitus. Calcium, vitamin D, and alendronate 40 mg per day were prescribed. Bisphosphonates have been used in the treatment of Charcot's foot because increased osteoclastic activity secondary to autonomic dysfunctions has been documented and vitamin D were started to prevent bisphosphonate induced osteomalacia.