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The study was focused on developing a continuous method to produce an alcohol mixture suitable to be used as a gasoline supplement. The immobilized column reactor with wood pulp fibers was successfully used for the continuous production of butanol and isopropanol using Clostridium beijerinckii DSM 6423. A sugar mixture (glucose, mannose, galactose, arabinose and xylose) representing lignocellulose hydrolysate was used as a substrate for the production of solvents. The effect of dilution rate on solvent production was studied during continuous operation. The maximum total solvent concentration of 11.99 g/l was obtained at a dilution rate of 0.16 h. The maximum solvent productivity (5.58 g/l h) was obtained at a dilution rate of 1.5 h. The maximum solvent yield of 0.45 g/g from sugar mixture was observed at 0.25 h. The system will be further used for the solvent production using wood hydrolysate as a substrate. [ABSTRACT FROM AUTHOR]