1. Inhibition of Bacillus licheniformis LMG 19409 from ropy cider by enterocin AS-48.
- Author
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Grande, Ma. J., Lucas, R., Abriouel, H., Valdivia, E., Omar, N. Ben, Maqueda, M., Martínez-Cañamero, M., and Gálvez, A.
- Subjects
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BACILLUS biotechnology , *BACILLUS (Bacteria) , *CIDER (Alcoholic beverage) , *BACTERIOCINS , *ANTIBACTERIAL agents , *MICROBIOLOGY - Abstract
Aims: To determine the activity of enterocin AS-48 against ropy-forming Bacillus licheniformis from cider. Methods and Results: Enterocin AS-48 was tested on B. licheniformis LMG 19409 from ropy cider in MRS-G broth, fresh-made apple juice and in two commercial apple ciders (A and B). Bacillus licheniformis was rapidly inactivated in MRS-G by 0·5 μg ml−1AS-48 and in fresh-made apple juice by 3 μg ml−1. Concentration-dependent inactivation of this bacterium in two commercial apple ciders (A and B) stored at 4, 15 and 30°C for 15 days was also demonstrated. Counts from heat-activated endospores in cider A plus AS-48 decreased very slowly. Application of combined treatments of heat (95°C) and enterocin AS-48 reduced the time required to achieved complete inactivation of intact spores in cider A to 4 min for 6 μg ml−1 and to 1 min for 12 μg ml−1. D and z values also decreased as the bacteriocin concentration increased. Conclusion: Enterocin AS-48 can inhibit ropy-forming B. licheniformis in apple cider and increase the heat sensitivity of spores. Significance and Impact of the Study: Results from this study support the potential use of enterocin AS-48 to control B. licheniformis in apple cider. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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