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Mitogen-transformed human peripheral blood lymphocytes and tonsil blasts were examined by rosette formation to detect the presence of membrane-bound immunoglobulin (Ig) and surface receptors for fixed IgG and fixed C3. In addition, the capacity of these cells to rosette with sheep erythrocytes was evaluated as a reaction characteristic of T lymphocytes. In order for clear morphological recognition of the rosetting transformed lymphocytes and the rosetting tonsil blasts a cytocentrifuge technique was developed and used in conjunction with autoradiography and/or with Romanowsky stains. Using these techniques and the culture methods described in this paper phytohaemagglutinin, pokeweed mitogen, streptococcal filtrates and purified protein derivative stimulated predominantly T cells in the peripheral blood of man. A minority of the transformed cells in these mitogen-stimulated cultures (<24%) did rosette with B lymphocyte markers and presumably represent a B-cell response. No significant differences were found between the T- or B-cell specificity of the mitogens investigated. Lymphoid preparations from tonsils excised from normal donors with recurrent tonsillitis were found to contain 6-15% lymphoblasts and the iarge majority of these cells formed rosettes with the B-cell markers, less than 20% of these lymphoblasts formed spontaneous sheep erythrocyte rosettes. Using a mixed rosetting technique a small proportion (<5%) of PHA-transformed cells and tonsil lymphoblasts were found to have combined sheep Fc or combined sheep C3 receptors. The investigation of B- and T-lymphocyte surface markers on mitogentransformed lymphocytes was extended to neopiastic lymphocyte populations and it was found that the majority of transformed cells (>70%) present in chronic lymphocytic leukaemia cultures stimulated with PHA after 6 days incubation were transformed T lymphocytes. [ABSTRACT FROM AUTHOR]

This paper describes the course of infection and certain pathological changes in thymus-deprived and normal CBA mice after oral infection with Trichinella spiralis. Greater morbidity was found among deprived mice and deaths due to trichiniasis occurred solely in this group. The local inflammatory response in the gut and skeletal muscles was defective in deprived animals: compared to normal mice it was slower to develop, contained fewer cells and was inadequately sustained. There was a striking paucity of tissue eosinophils and this was correlated with failure of deprived mice to develop a blood eosinophilia. The parasites appeared to persist longer in the gut of deprived animals and progressively more parasites were recovered from the carcasses of these mice at later stages of the infection. The present findings indicate that thymus-dependent cell populations are implicated in the host response to T. spiralis; and some possible modes of action of T cells are discussed. [ABSTRACT FROM AUTHOR]

Immunosurveillance requires the migration of lymphocytes and their activation to induce proliferation and effector function. Effective immunity requires an optimal supply of nutrients to lymphocytes. Cells contain nutrient sensing apparatus such as adenosine 5′-monophosphate-activated protein kinase (AMPK) that surveys intracellular ATP levels. Immunity declines during ageing and one possibility is that the energy balance may be altered in old lymphocytes. This paper summarizes recent data identifying a convergence of senescence and nutrient signalling pathways in lymphocytes that inhibit both T cell and natural killer (NK) cell function during ageing. Significantly, these pathways can be inhibited to enhance the activity of these cells. [ABSTRACT FROM AUTHOR]

The article presents a notice of redundant publication on topics related to clinical and experimental immunology. The text and data of the paper "Peptide immunization in humans: a combined CD8+ T cell-targeted vaccine restimulates the memory CD4 T cell response but fails to induce cytoxic T lymphocytes (CTL)" is replicated without acknowledgement in the subsequent paper. The data indicate that the CTL peptide failed to stimulate a cytotoxic immune response.

The article presents information on various research papers published in journals related to dermatology. In one of the research papers, a missense mutation in SART3 gene was reported in a six-generation Chinese pedigree with disseminated superficial actinic porokeratosis. SART3 gene encodes a tumor-rejection antigen recognized by cytotoxic T lymphocytes, which may be associated with immunosuppression and malignant transformation. In another research, a novel therapy for wounds in rheumatic diseases patients is reported. Including those with rheumatoid arthritis or systemic sclerosis, who had wounds with exposed bones were treated either with the standard procedure or with a newly developed experimental procedure.

Responds to Jorge Casseb's comment on the authors' paper on the interaction of human T cell leukemia type I with liver disease in hepatitis C virus-infected patients. Effect of excess pro-inflammatory cytokine production; Preservation of cellular immunity in infected host; Liver cancer without induction of chronic hepatitis.

The article presents abstracts of papers related to renal transplantation pathomechanisms and basic science including "Deleterious Effect of Direct and Indirectly-Primed Alloreactive T-Cells After Long Time After Transplantation," by Oriol Bestard and colleages, "The Suppressor Properties of CD8+CD28-Cells in Mixed Lymphocyte Culture (MLC)," by Anna Korecka and colleagues, and "Renal Expression of Melanocortin-1 Receptor in the Rat Ischemia-Reperfusion Injury," by YoungSook Lee and colleagues.

Comments on Cynthia Boschi-Pinto, Sherri Stuver, Akihiko Okayama and Nancy Mueller's paper on the interaction of human T cell leukemia type I with liver disease in hepatitis C virus (HCV)-infected patients. Increase in risk for liver disease; Capacity to induce T cell proliferation and produce interferon-gamma; Capacity of T lymphocytes to respond to HCV-infected hepatocytes.

Presents an abstract of the research paper 'Cutaneous Lymphocyte-Associated Antigen Positive T-Cells Infiltrate Early and Specifically in Evolving Psoriatic Plaques,' by S. Davison, A. Ballsdon, M. Allen and J. Barker presented during the annual Meeting of the British Association of Dermatologists in Edinburgh, Scotland.

Presents an abstract of the research paper 'Identification and Isolation of Skin-Homing Melanocyte-Specific Cytotoxic T Lymphocytes From Individuals With Vitiligo,' by G.S. Ogg, P.R. Dunbar, P. Romero, J.L. Chen and V. Cerundolo presented at the Annual Meeting of the British Association of Dermatologists in Edinburgh, Scotland.