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The article presents a study on the role of bi-cistronic baculovirus/T in larvae expression system in producing recombinant E2 glycoprotein (rE2). The study indicates that the larvae expression system can secrete substantial levels of rE2 glycoprotein to the hemolymph. Moreover, it also determines that the system can help distinguish infected and uninfected larvae and reduces target protein degradation.

A green fluorescent protein ( gfp) gene was ligated to the Lactobacillus reuteri-specific nisin-inducible expression-secretion vector pNIES, generating a pNIES-GFP vector capable of secreting the cloned gene as a GFP-fusion protein with fluorescent activity. To develop this system as a live vehicle carrying the heat-stable enterotoxin (ST) and heat-labile enterotoxin B (LTB) of the enterotoxigenic Escherichia coli (ETEC), a recombinant 5′- ST- LT B-3′ DNA fragment was cloned into pNIES-GFP. The resulting L. reuteri/pNIES-GFP:STLTB system was found to possess the capability of adhering to the mice gut, secreting GFP:STLTB product at 0.14 and 0.026 pgcell−1 under induced and noninduced conditions, respectively. Further analysis of the GFP:STLTB product confirmed its ganglioside-binding ability, LTB antigenicity and relative freedom from the ST-associated toxicity, making it suitable for use as an oral vaccine in mice. Oral inoculation of the L. reuteri/pNIES-GFP:STLTB culture in mice elicited significant ( P<0.01) serum IgG and mucosal IgA antibodies against the STLTB antigen. These immunized mice were subsequently challenged with ETEC and showed full protection against the fluid influx response in the gut. This is the first report of using L. reuteri as a vaccine carrier to induce complete immunologic protection against ETEC. [ABSTRACT FROM AUTHOR]

The article discusses the study, which aims to determine the functional expression of a fungal immunomodulatory protein (FIP) from Song-Shan Lingzhi in Sf21 insect cells. The study involves the fusing of a recombinant FIP-gts and 6His-tag at its C-terminus, which was expressed in Sf21 insect cells by the baculovirus expression system, to produce active FIP-gts for evaluation of oral administration. Key information regarding the study are further discussed.

The article discusses the construction of nissin-controlled gene expression system in Lactococcus reuteri. The system was constructed by ligating nisA promoter and nisRK DNA fragments into the Escherichia coli-Lb. reuteri shuttle vector pSTE32. The chimerical plasmid was capable of expressing the heterologous amylase gene under nissin induction.