1. Experimental strategies for studying transcription factor–DNA binding specificities.
- Author
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Geertz, Marcel and Maerk, Sebastian J.
- Subjects
- *
DNA-ligand interactions , *TRANSCRIPTION factors , *GENETIC regulation , *PROTEIN-protein interactions , *ELECTROPHORESIS - Abstract
Specific binding of transcription factors (TFs) determines in a large part the connectivity of gene regulatory networks as well as the quantitative level of gene expression. A multiplicity of both experimental and computational methods is currently used to discover and characterize the underlying TF–DNA interactions. Experimental methods can be further subdivided into in vitro- and in vivo-based approaches, each accenting different aspects of TF-binding events. In this review we summarize the flexibility and performance of a selection of both types of experimental methods. In conclusion, we argue that a serial combination of methods with different throughput and data type constitutes an optimal experimental strategy. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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