1. Clustering malignant cell states using universally variable genes.
- Author
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Yoon, Sang-Ho and Nam, Jin-Wu
- Subjects
- *
CANCER cells , *RNA sequencing , *GENE expression , *GENES , *FEATURE selection - Abstract
Single-cell RNA sequencing (scRNA-seq) has revealed important insights into the heterogeneity of malignant cells. However, sample-specific genomic alterations often confound such analysis, resulting in patient-specific clusters that are difficult to interpret. Here, we present a novel approach to address the issue. By normalizing gene expression variances to identify universally variable genes (UVGs), we were able to reduce the formation of sample-specific clusters and identify underlying molecular hallmarks in malignant cells. In contrast to highly variable genes vulnerable to a specific sample bias, UVGs led to better detection of clusters corresponding to distinct malignant cell states. Our results demonstrate the utility of this approach for analyzing scRNA-seq data and suggest avenues for further exploration of malignant cell heterogeneity. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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