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Protein arginine methyltransferase 1 (PRMT1) is involved in multiple cellular functions including proliferation and differentiation. Although PRMT1 is expressed in vascular endothelial cells (ECs), which are responsible for angiogenesis during embryonic development, its role has remained elusive. In this study, we generated endothelial-specific prmt1-knockout (Prmt1-ECKO) mice, and found that they died before embryonic day 15. The superficial temporal arteries in these embryos were poorly perfused with blood, and wholemount 3D imaging revealed dilated and segmentalized luminal structures in Prmt1-ECKO fetuses in comparison with those of controls. Our findings provide evidence that PRMT1 is important for embryonic vascular formation. [ABSTRACT FROM AUTHOR]

The article presents a study on the detection of the catalysis of choline and phosphatidic acid (PA) by phospholipase D (PLD) with the use of matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight mass spectrometry (MALDI-QIT-TOF/MS) with 9-aminoacridine matrix. Topics discussed include detection of choline and PA as a reaction product of phosphatidylcholine hydrolysis, enzymatic properties of PLD, and choline as a precursor of the neurotransmitter acetylcholine.