1. Potential involvement of drought-induced Ran GTPase CLRan1 in root growth enhancement in a xerophyte wild watermelon.
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Akashi, Kinya, Yoshimura, Kazuya, Kajikawa, Masataka, Hanada, Kouhei, Kosaka, Rina, Kato, Atsushi, Katoh, Akira, Nanasato, Yoshihiko, Tsujimoto, Hisashi, and Yokota, Akiho
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GUANOSINE triphosphatase ,EFFECT of drought on plants ,WATERMELONS - Abstract
Enhanced root growth is known as the survival strategy of plants under drought. Previous proteome analysis in drought-resistant wild watermelon has shown that Ran GTPase, an essential regulator of cell division and proliferation, was induced in the roots under drought. In this study, two cDNAs were isolated from wild watermelon,CLRan1andCLRan2, which showed a high degree of structural similarity with those of other plant Ran GTPases. Quantitative RT-PCR and promoter-GUS assays suggested thatCLRan1was expressed mainly in the root apex and lateral root primordia, whereasCLRan2was more broadly expressed in other part of the roots. Immunoblotting analysis confirmed that the abundance of CLRan proteins was elevated in the root apex region under drought stress. Transgenic Arabidopsis overexpressing CLRan1 showed enhanced primary root growth, and the growth was maintained under osmotic stress, indicating thatCLRan1functions as a positive factor for maintaining root growth under stress conditions. Ran GTPase (CLRan1), which is expressed in the roots of drought-resistant wild watermelon, functions as a positive factor for maintaining root growth under osmotic stress. [ABSTRACT FROM PUBLISHER]
- Published
- 2016
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