1. Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes.
- Author
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Trachtulec, Zdeněk, Mihola, Ondrej, Vlcek, Cestmír, Himmelbauer, Heinz, Pačes, Václav, and Forejt, Jiří
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INFERTILITY ,GENES ,MOLECULAR cloning ,MOLECULAR genetics ,GENE mapping ,ANIMAL genome mapping ,BIOLOGICAL evolution ,MICE ,GENETICS - Abstract
The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and someMus musculus musculusmice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of theHst1gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow theHst1region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes forHst1by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs. © 2005 The Linnean Society of London,Biological Journal of the Linnean Society, 2005,84, 637–641. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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