1. PGC-1beta down-regulation is associated with reduced ERRalpha activity and MCAD expression in skeletal muscle of senescence-accelerated mice.
- Author
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Rodríguez-Calvo R, Jové M, Coll T, Camins A, Sánchez RM, Alegret M, Merlos M, Pallàs M, Laguna JC, Vázquez-Carrera M, Rodríguez-Calvo, Ricardo, Jové, Mireia, Coll, Teresa, Camins, Antoni, Sánchez, Rosa M, Alegret, Marta, Merlos, Manuel, Pallàs, Mercè, Laguna, Juan C, and Vázquez-Carrera, Manuel
- Abstract
Mitochondrial dysfunction is involved in the development of aging. Here, we examined the effect of aging on the skeletal muscle expression of two isoforms of the transcriptional peroxisome proliferator-activated receptor gamma (PPARgamma) coactivator-1 (PGC-1) in an experimental murine model of accelerated aging, the senescence-accelerated mouse (SAM). The senescence-accelerated prone mice (SAM-P8) showed no changes in PGC-1alpha, but a decrease in PGC-1beta expression (52% reduction, p <.001) was observed compared to the senescence-accelerated resistant mice (SAM-R1). In agreement with the proposed role of PGC-1beta as an estrogen-related receptor (ERR) protein ligand, the expression of the ERRalpha target gene medium-chain acyl-coenzyme A dehydrogenase was strongly suppressed (85%, p <.001) in SAM-P8. The decrease in the expression of medium-chain acyl-coenzyme A dehydrogenase was consistent with the reduction in ERRalpha DNA-binding activity of SAM-P8. These findings indicate that the age-mediated decrease in PGC-1beta expression in SAM-P8 skeletal muscle affects the expression of genes involved in mitochondrial fatty acid oxidation. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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