1. Nigrostriatal Proteomics of Cypermethrin-Induced Dopaminergic Neurodegeneration: Microglial Activation-Dependent and -Independent Regulations.
- Author
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Singh, Anand Kumar, Tiwari, Manindra Nath, Dixit, Anubhuti, Upadhyay, Ghanshyam, Patel, Devendra Kumar, Singh, Dhirendra, Prakash, Om, and Singh, Mahendra Pratap
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PHYSIOLOGICAL effects of insecticides , *PYRETHROIDS , *PROTEOMICS , *GENE expression , *NEURODEGENERATION , *DOPAMINERGIC neurons , *MITOGEN-activated protein kinases , *HEAT shock proteins - Abstract
The study aimed to identify the differentially expressed nigrostriatal proteins in cypermethrin-induced neurodegeneration and to investigate the role of microglial activation therein. Proteomic approaches were used to identify the differentially expressed proteins. Microglial activation, tyrosine hydroxylase immunoreactivity (TH-IR), dopamine content, and neurobehavioral changes were measured according to the standard procedures. The expressions of α-internexin intermediate filament (α-IIF), ATP synthase D chain (ATP-SD), heat shock protein (Hsp)-70, truncated connexin-47, Hsp-60, mitogen-activated protein kinase-activated kinase-5, nicotinamide adenine dinucleotide dehydrogenase 24k chain precursor, platelet-activating factor acetyl hydrolase 1b-α2 (PAF-AH 1b-α2), and synaptosomal-associated protein-25 (SNAP-25) were altered in the substantia nigra and nicotinamide adenine dinucleotide- specific isocitrate dehydrogenase, phosphatidylethanolamine-binding protein-1, prohibitin, protein disulfide isomerase-endoplasmic reticulum 60 protease, stathmin, and ubiquitin-conjugating enzyme in the striatum along with motor impairment, decreased dopamine and TH-IR, and increased microglial activation after cypermethrin exposure. Minocycline restored α-IIF, ATP-SD chain, truncated connexin-47, Hsp-60, PAF-AH 1b-α2, stathmin and SNAP-25 expressions, motor impairment, dopamine, TH-IR, and microglial activation. The results suggest that cypermethrin produces microglial activation-dependent and -independent changes in the expression patterns of the nigrostriatal proteins leading to dopaminergic neurodegeneration. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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