9 results on '"Ulvskov, Peter"'
Search Results
2. Evidence for land plant cell wall biosynthetic mechanisms in charophyte green algae.
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Mikkelsen, Maria D., Harholt, Jesper, Ulvskov, Peter, Johansen, Ida E., Fangel, Jonatan U., Doblin, Monika S., Bacic, Antony, and Willats, William G. T.
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PLANT cell walls ,GREEN algae ,BIOSYNTHESIS ,BIOCHEMICAL engineering ,CHAROPHYTA - Abstract
Background and Aims The charophyte green algae (CGA) are thought to be the closest living relatives to the land plants, and ancestral CGA were unique in giving rise to the land plant lineage. The cell wall has been suggested to be a defining structure that enabled the green algal ancestor to colonize land. These cell walls provide support and protection, are a source of signalling molecules, and provide developmental cues for cell differentiation and elongation. The cell wall of land plants is a highly complex fibre composite, characterized by cellulose cross-linked by non-cellulosic polysaccharides, such as xyloglucan, embedded in a matrix of pectic polysaccharides. How the land plant cell wall evolved is currently unknown: early-divergent chlorophyte and prasinophyte algae genomes contain a low number of glycosyl transferases (GTs), while land plants contain hundreds. The number of GTs in CGA is currently unknown, as no genomes are available, so this study sought to give insight into the evolution of the biosynthetic machinery of CGA through an analysis of available transcriptomes. Methods Available CGA transcriptomes were mined for cell wall biosynthesis GTs and compared with GTs characterized in land plants. In addition, gene cloning was employed in two cases to answer important evolutionary questions. Key Results Genetic evidence was obtained indicating that many of the most important core cell wall polysaccharides have their evolutionary origins in the CGA, including cellulose, mannan, xyloglucan, xylan and pectin, as well as arabino-galactan protein. Moreover, two putative cellulose synthase-like D family genes (CSLDs) from the CGA species Coleochaete orbicularis and a fragment of a putative CSLA/K-like sequence from a CGA Spirogyra species were cloned, providing the first evidence that all the cellulose synthase/-like genes present in early-divergent land plants were already present in CGA. Conclusions The results provide new insights into the evolution of cell walls and support the notion that the CGA were pre-adapted to life on land by virtue of the their cell wall biosynthetic capacity. These findings are highly significant for understanding plant cell wall evolution as they imply that some features of land plant cell walls evolved prior to the transition to land, rather than having evolved as a result of selection pressures inherent in this transition. [ABSTRACT FROM PUBLISHER]
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- 2014
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3. Toward Stable Genetic Engineering of Human O-Glycosylation in Plants.
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Zhang Yang, Bennett, Eric P., Jargensen, Bodil, Drew, Damian P., Arigi, Emma, Mandel, Ulla, Ulvskov, Peter, Levery, Steven B., Clausen, Henrik, and Petersen, Bent L.
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GENETIC engineering ,GLYCOSYLATION ,PLANTS ,GENETIC recombination ,ESTERIFICATION - Abstract
Glycosylation is the most abundant and complex posttranslational modification to be considered for recombinant production of therapeutic proteins. Mucin-type (N-acetylgalactosamine [GalNAc]-type) O-glycosylation is found in eumetazoan cells but absent in plants and yeast, making these cell types an obvious choice for de novo engineering of this O-glycosylation pathway. We previously showed that transient implementation of O-glycosylation capacity in plants requires introduction of the synthesis of the donor substrate UDP-GalNAc and one or more polypeptide GalNAc-transferases for incorporating GalNAc residues into proteins. Here, we have stably engineered O-glycosylation capacity in two plant cell systems, soil-grown Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum) Bright Yellow-2 suspension culture cells. Efficient GalNAc O-glycosylation of two stably coexpressed substrate O-glycoproteins was obtained, but a high degree of proline hydroxylation and hydroxyproline-linked arabinosides, on a mucin (MUC1)-derived substrate, was also observed. Addition of the prolyl 4-hydroxylase inhibitor 2,2-dipyridyl, however, effectively suppressed proline hydroxylation and arabinosylation of MUC1 in Bright Yellow-2 cells. In summary, stably engineered mammalian type O-glycosylation was established in transgenic plants, demonstrating that plants may serve as host cells for the production of recombinant O-glycoproteins. However, the present stable implementation further strengthens the notion that elimination of endogenous posttranslational modifications may be needed for the production of protein therapeutics. [ABSTRACT FROM AUTHOR]
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- 2012
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4. Mechanical Properties of Plant Cell Walls Probed by Relaxation Spectra.
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Hansen, Steen Laugesen, Ray, Peter Martin, Karlsson, Anders Ola, Jorgensen, Bodil, Borkhardt, Bernhard, Petersen, Bent Larsen, and Ulvskov, Peter
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PLANT cell walls ,POLYMERS ,BIOMECHANICS ,PLANT mechanics ,COMPUTER software - Abstract
Transformants and mutants with altered cell wall composition are expected to display a biomechanical phenotype due to the structural role of the cell wall. It is often quite difficult, however, to distinguish the mechanical behavior of a mutant's or transformant's cell walls from that of the wild type. This may be due to the plant's ability to compensate for the wall modification or because the biophysical method that is often employed, determination of simple elastic modulus and breakstrength, lacks the resolving power necessary for detecting subtle mechanical phenotypes. Here, we apply a method, determination of relaxation spectra, which probes, and can separate, the viscoelastic properties of different cell wall components (i.e. those properties that depend on the elastic behavior of load-bearing wall polymers combined with viscous interactions between them). A computer program, BayesRelax, that deduces relaxation spectra from appropriate theological measurements is presented and made accessible through a Web interface. BayesRelax models the cell wall as a continuum of relaxing elements, and the ability of the method to resolve small differences in cell wall mechanical properties is demonstrated using tuber tissue from wild-type and transgenic potatoes (Solanum tuberosum) that differ in rhamnogalacturonan I side chain structure. [ABSTRACT FROM AUTHOR]
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- 2011
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5. Metabolomic, Transcriptional, Hormonal, and Signaling Cross-Talk in Superroot2.
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Morant, Marc, Ekstrøm, Claus, Ulvskov, Peter, Kristensen, Charlotte, Rudemo, Mats, Olsen, Carl Erik, Hansen, Jørgen, Jørgensen, Kirsten, Jørgensen, Bodil, Møller, Birger Lindberg, and Bak, Søren
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AUXIN ,HOMEOSTASIS ,PLANT hormones ,PLANT growth ,PLANT development ,GLUCOSINOLATES - Abstract
Auxin homeostasis is pivotal for normal plant growth and development. The superroot2 (sur2) mutant was initially isolated in a forward genetic screen for auxin overproducers, and SUR2 was suggested to control auxin conjugation and thereby regulate auxin homeostasis. However, the phenotype was not uniform and could not be described as a pure high auxin phenotype, indicating that knockout of CYP83B1 has multiple effects. Subsequently, SUR2 was identified as CYP83B1, a cytochrome P450 positioned at the metabolic branch point between auxin and indole glucosinolate metabolism. To investigate concomitant global alterations triggered by knockout of CYP83B1 and the countermeasures chosen by the mutant to cope with hormonal and metabolic imbalances, 10-day-old mutant seedlings were characterized with respect to their transcriptome and metabolome profiles. Here, we report a global analysis of the sur2 mutant by the use of a combined transcriptomic and metabolomic approach revealing pronounced effects on several metabolic grids including the intersection between secondary metabolism, cell wall turnover, hormone metabolism, and stress responses. Metabolic and transcriptional cross-talks in sur2 were found to be regulated by complex interactions between both positively and negatively acting transcription factors. The complex phenotype of sur2 may thus not only be assigned to elevated levels of auxin, but also to ethylene and abscisic acid responses as well as drought responses in the absence of a water deficiency. The delicate balance between these signals explains why minute changes in growth conditions may result in the non-uniform phenotype. The large phenotypic variation observed between and within the different surveys may be reconciled by the complex and intricate hormonal balances in sur2 seedlings decoded in this study. [ABSTRACT FROM PUBLISHER]
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- 2010
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6. Arabidopsis thaliana RGXT1 and RGXT2 Encode Golgi-Localized (1,3)-α-D-Xylosyltransferases Involved in the Synthesis of Pectic Rhamnogalacturonan-II.
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Egelund, Jack, Petersen, Bent Larsen, Motawia, Mohammed Saddik, Damager, Iben, Faik, Ahmed, Olsen, Carl Erik, Ishii, Tadashi, Clausen, Henrik, Ulvskov, Peter, and Geshia, Naomi
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ARABIDOPSIS thaliana ,GLYCOSYLTRANSFERASES ,PLANT cell walls ,MEMBRANE proteins ,DISACCHARIDES ,STEREOCHEMISTRY ,POLYSACCHARIDES ,BIOSYNTHESIS - Abstract
Two homologous plant-specific Arabidopsis thaliana genes, RGXT1 and RGXT2, belong to a new family of glycosyltransferases (CAZy GT-family-77) and encode cell wall (1,3)-α-D-xylosyltransferases. The deduced amino acid sequences contain single transmembrane domains near the N terminus, indicative of a type II membrane protein structure. Soluble secreted forms of the corresponding proteins expressed in insect cells showed xylosyltrensferase activity, transferring D-xylose from UDP-α-xylose to L-fucose. The disaccharide product was hydrolyzed by α-xylosidase, whereas no reaction was catalyzed by β-xylosidase. Furthermore, the regio- and stereochemistry of the methyl xylosyl-fucoside was determined by nuclear magnetic resonance to be an α-(1,3) linkage, demonstrating the isolated glycosyltransferases to be (1,3)-α-D-xylosyltransferases. This particular linkage is only known in rhamnogalacturonan-II, a complex polysaccharide essential to vascular plants, and is conserved across higher plant families. Rhamnogalacturonan-II isolated from both RGXT1 and RGXT2 T-DNA insertional mutants functioned as specific acceptor molecules in the xylosyltransferase assay. Expression of RGXT1- and RGXT2-enhanced green fluorescent protein constructs in Arabidopsis revealed that both fusion proteins were targeted to a Brefeldin A-sensitive compartment and also colocalized with the Golgi marker dye BODIPY TR ceramide, consistent with targeting to the Golgi apparatus. Taken together, these results suggest that RGXT1 and RGXT2 encode Golgi-localized (1,3)-α-D-xylosyltransferases involved in the biosynthesis of pectic rhamnogalacturonan-II. [ABSTRACT FROM AUTHOR]
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- 2006
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7. If Homogalacturonan Were a Side Chain of Rhamnogalacturonan I. Implications for Cell Wall Architecture.
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Vincken, Jean-Paul, Schols, Henk A., Oomen, Ronald J.F.J., McCann, Maureen C., Ulvskov, Peter, Voragen, Alphons G.J., and Visser, Richard G.F.
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PECTINS ,CITRUS fruit by-products ,PLANT cell walls - Abstract
Summarizes the large amount of data on pectin structure. Model for the macromolecular structure of pectin; Ideas on how pectins are integrated into the plant cell wall; Constituent polymers and primary structure of pectins.
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- 2003
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8. Immunoaffinity Purification of Indole-3-acetamide Using Monoclonal Antibodies.
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Ulvskov, Peter, Marcussen, Jan, Rajagopal, Ranganatha, Prinsen, Els, Rudelsheim, Patrick, and Onckelen, Henri Van
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- 1987
9. New Polysaccharide with a Long Evolutionary History.
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Ulvskov, Peter and Harholt, Jesper
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POLYSACCHARIDES , *CELLULOSE synthase , *SCIENTIFIC method , *PLANT cell walls , *XYLANS - Abstract
Neofunctionalization could have occurred when the modern rosette-shaped cellulose synthase complex evolved near the base of the charophytic algae, making linear terminal complex cellulose synthases redundant. Cellulose synthase has a prokaryotic origin, and the arabinoglucan synthase, AGlcS, is strikingly similar to the prokaryotic homologs as well as cellulose synthases in fungi, oomycetes, and even a few animals. The discovery of the mixed-linkage arabinoglucan presents us with the only known polysaccharide synthesized by a member of the cellulose synthase superfamily featuring both hexosyl and pentosyl residues in its backbone. [Extracted from the article]
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- 2018
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