1. Pro-angiogenic activity of isofuran.
- Author
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Jingxia Zhang, Xiangjiang Wang, Zhiping Lin, and Tang Zhu
- Subjects
- *
PROTEIN kinases , *RYANODINE receptors , *ENDOTHELIAL cells - Abstract
Isofurans (IsoFs) are a series of novel discovered lipid peroxidation products. This study focused on the investigation of the angiogenic property of IsoF. MTT stain assay indicated that 1 μm IsoF had the most bioactivity in rat brain endothelial cells (RBECs). IsoF significantly promoted cellular proliferation and migration and remarkably decreased staurosporine-induced apoptosis by TUNEL assay in the RBECs. It successfully up-regulated rat aortic vascularization and choroid explant sprouting, extracellular regulated protein kinases (ERK) 1/2, and triggered calcium release. RT-PCR examination indicated that IsoF up-regulated tumor necrosis factor (NF)α, angiopoietin-1 receptor (Tie2), and vascular endothelial growth factor (VEGF)-A, but did not interfere with caspase 2 and VEGF-C in the RBECs. IsoF has pro-angiogenic activity. Calcium release and ERK1/2 phosphorylation may be involved in the signaling of the IsoF-induced up-regulation of TNF α, Tie2, and VEGF-A, which could be the molecular mechanism of the pro-angiogenic activity of the IsoF. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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