1. Effect of Priming With Seasonal Influenza A(H3N2) Virus on the Prevalence of Cross-Reactive Hemagglutination-Inhibition Antibodies to Swine-Origin A(H3N2) Variants.
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Feng Liu, Veguilla, Vic, Gross, F. Liaini, Gillis, Eric, Rowe, Thomas, Xiyan Xu, Tumpey, Terrence M., Katz, Jacqueline M., Levine, Min Z., Xiuhua Lu, Liu, Feng, Xu, Xiyan, and Lu, Xiuhua
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INFLUENZA A virus, H3N2 subtype ,BLOOD agglutination ,SWINE diseases ,ANTIBODY formation ,DISEASE prevalence ,INFLUENZA epidemiology ,ANIMAL experimentation ,ANTIGEN-antibody reactions ,EPIDEMIOLOGICAL research ,HEMAGGLUTINATION tests ,INFLUENZA ,MAMMALS ,RESEARCH funding ,SWINE ,VIRAL antibodies ,VIRAL antigens ,ORTHOMYXOVIRUS infections - Abstract
Background: Recent outbreaks of swine-origin influenza A(H3N2) variant (H3N2v) viruses have raised public health concerns. Previous studies indicated that older children and young adults had the highest levels of hemagglutination-inhibition (HI) antibodies to 2010-2011 H3N2v viruses. However, newly emerging 2013 H3N2v have acquired antigenic mutations in the hemagglutinin at amino acid position 145 (N145K/R). We estimated the levels of serologic cross-reactivity among humans primed with seasonal influenza A(H3N2) (sH3N2), using postinfection ferret antisera. We also explored age-related HI antibody responses to 2012-2013 H3N2v viruses.Methods: Human and ferret antisera were tested in HI assays against 1 representative 2012 H3N2v (145N) and 2 2013 H3N2v (145K/R) viruses, together with 9 sH3N2 viruses circulating since 1968.Results: Low levels of cross-reactivity between the H3N2v and sH3N2 viruses from the 1970s-1990s were observed using postinfection ferret antisera. The overall seroprevalence among the sH3N2-primed population against 2012-2013 H3N2v viruses was >50%, and age-related seroprevalence was observed. Seroprevalence was significantly higher to 2013 H3N2v than to 2012 H3N2v viruses among some children likely to have been primed with A/Sydney/5/97-like (145K) or A/Wuhan/359/95-like viruses (145K).Conclusions: A single substitution (N145K/R) was sufficient to affect seropositivity to H3N2v viruses in some individuals. Insight into age-related antibody responses to newly emerging H3N2v viruses is critical for risk assessment and pandemic preparedness. [ABSTRACT FROM AUTHOR]- Published
- 2017
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