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The plant hormone abscisic acid (ABA) regulates many aspects of plant development and the stress response. The intracellular E3 ligase SDIR1 (SALT- AND DROUGHT-INDUCED REALLY INTERESTING NEW GENE FINGER1) plays a key role in ABA signaling, regulating ABA -related seed germination and the stress response. In this study, we found that SDIR1 is localized on the endoplasmic reticulum membrane in Arabidopsis thaliana. Using cell biology, molecular biology, and biochemistry approaches, we demonstrated that SDIR1 interacts with and ubiquitinates its substrate, SDIRIP1 (SDIR1-INTERACTING PROTEIN1), to modulate SDIRIP1 stability through the 26S proteasome pathway. SDIRIP1 acts genetically downstream of SDIR1 in ABA and salt stress signaling. In detail, SDIRIP1 selectively regulates the expression of the downstream basic region/leucine zipper motif transcription factor gene ABA-INSENSITIVE5 , rather than ABA-RESPONSIVE ELEMENTS BINDING FACTOR3 (ABF3) or ABF4 , to regulate ABA -mediated seed germination and the plant salt response. Overall, the SDIR1/SDIRIP1 complex plays a vital role in ABA signaling through the ubiquitination pathway. [ABSTRACT FROM AUTHOR]

We employ a sample of 135 873 RR Lyrae stars (RRLs) with precise photometric-metallicity and distance estimates from the newly calibrated P –ϕ31– R 21–[Fe/H] and Gaia G band P – R 21–[Fe/H] absolute magnitude–metallicity relations of Li et al. combined with available proper motions from Gaia EDR3, and 6955 systemic radial velocities from Gaia DR3 and other sources, in order to explore the chemistry and kinematics of the halo of the Milky Way (MW). This sample is ideally suited for characterization of the inner- and outer-halo populations of the stellar halo, free from the bias associated with spectroscopically selected probes, and for estimation of their relative contributions as a function of Galactocentric distance. The results of a Gaussian mixture model analysis of these contributions are broadly consistent with other observational studies of the halo, and with expectations from recent MW simulation studies. We apply the hdbscan clustering method to the specific energies and cylindrical actions (E , Jr, Jϕ, J z ), identifying 97 dynamically tagged groups (DTGs) of RRLs, and explore their associations with recognized substructures of the MW. The precise photometric-distance determinations (relative distance errors on the order of 5 per cent or better), and the resulting high-quality determination of dynamical parameters, yield highly statistically significant (low) dispersions of [Fe/H] for the stellar members of the DTGs compared to random draws from the full sample, indicating that they share common star-formation and chemical histories, influenced by their birth environments. [ABSTRACT FROM AUTHOR]

We use 3653 (2661 RRab, 992 RRc) RR Lyrae stars (RRLs) with 7D (3D position, 3D velocity, and metallicity) information selected from Sloan Digital Sky Survey, Large Sky Area Multi-Object Fiber Spectroscopic Telescope, and Gaia EDR3, and divide the sample into two Oosterhoff groups (Oo I and Oo II) according to their amplitude–period behaviour in the Bailey diagram. We present a comparative study of these two groups based on chemistry, kinematics, and dynamics. We find that Oo I RRLs are relatively more metal-rich, with predominately radially dominated orbits and large eccentricities, while Oo II RRLs are relatively more metal-poor, and have mildly radially dominated orbits. The Oosterhoff dichotomy of the Milky Way's halo is more apparent for the inner-halo region than for the outer-halo region. Additionally, we also search for this phenomenon in the haloes of the two largest satellite galaxies, the Large and Small Magellanic clouds, and compare over different bins in metallicity. We find that the Oosterhoff dichotomy is not immutable, and varies based on position in the Galaxy and from galaxy to galaxy. We conclude that the Oosterhoff dichotomy is the result of a combination of stellar and galactic evolution, and that it is much more complex than the dichotomy originally identified in Galactic globular clusters. [ABSTRACT FROM AUTHOR]

DNA methylation is a conserved epigenetic modification that is typically associated with silencing of transposable elements and promoter methylated genes. However, some DNA-methylated loci are protected from silencing, allowing transcriptional flexibility in response to environmental and developmental cues. Through a genetic screen in Arabidopsis (Arabidopsis thaliana), we uncovered an antagonistic relationship between the MICRORCHIDIA (MORC) protein and the IMITATION SWITCH (ISWI) complex in regulating the DNA-methylated SUPPRESSOR OF DRM1 DRM2 CMT3 (SDC) reporter. We demonstrate that components of the plant-specific ISWI complex, including CHROMATIN REMODELING PROTEIN11 (CHR11), CHR17, DDT-RELATED PROTEIN4 (DDR4), and DDR5, function to partially derepress silenced genes and transposable elements (TEs), through their function in regulating nucleosome distribution. This action also requires the known transcriptional activator DNAJ proteins, providing a mechanistic link between nucleosome remodeling and transcriptional activation. Genome-wide studies revealed that DDR4 causes changes in nucleosome distribution at numerous loci, a subset of which is associated with changes in DNA methylation and/or transcription. Our work reveals a mechanism for balancing transcriptional flexibility and faithful silencing of DNA-methylated loci. As both ISWI and MORC family genes are widely distributed across plant and animal species, our findings may represent a conserved eukaryotic mechanism for fine-tuning gene expression under epigenetic regulation. The DDT-RELATED PROTEIN4–IMITATION SWITCH nucleosome remodeler activates gene and transposable element expression through altered nucleosome distribution. [ABSTRACT FROM AUTHOR]

Based on a large spectroscopic sample of ∼4300 RR Lyrae stars with metallicity, systemic radial velocity, and distance measurements, we present a detailed analysis of the chemical and kinematic properties of the Galactic halo. Using this sample, the metallicity distribution function (MDF) as a function of r and the velocity anisotropy parameter β profiles (for different metallicity populations) are derived for the stellar halo. Both the chemical and kinematic results suggest that the Galactic halo is composed of two distinct parts, the inner halo and outer halo. The cutoff radius (∼30 kpc) is similar to the previous break radius found in the density distribution of the stellar halo. We find that the inner part is dominated by a metal-rich population with extremely radial anisotropy (β ∼ 0.9). These features are in accordance with those of ' Gaia -Enceladus-Sausage' (GES) and we attribute this inner halo component as being dominantly composed of stars deposited from this ancient merged satellite. We find that GES probably has a slightly negative metallicity gradient. The metal-poor populations in the inner halo are characterized as a long tail in MDF with an anisotropy of β ∼ 0.5, which is similar to that of the outer part. The MDF for the outer halo is very broad with several weak peaks and the value of β is around 0.5 for all metallicities. [ABSTRACT FROM AUTHOR]