1. Prevalence and characteristics of ESBL-producing Escherichia coli in clinically healthy pigs: implications for antibiotic resistance spread in livestock.
- Author
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Oliveira, Rúzivia Pimentel, da Silva, Juliana Soares, da Silva, Giarlã Cunha, Rosa, Jéssica Nogueira, Bazzolli, Denise Mara Soares, and Mantovani, Hilario C
- Subjects
DRUG resistance in bacteria ,MOBILE genetic elements ,BETA-lactamase inhibitors ,ESCHERICHIA coli ,BETA lactamases ,SWINE ,DRUG resistance in microorganisms ,LIVESTOCK - Abstract
Aim This study aimed to compare and characterize the resistance profile and the presence of extended-spectrum beta-lactamase (ESBL) related genes in Escherichia coli isolated from healthy finishing pigs fed with or without antibiotics in their diets. Methods and results A total of 27 ceftiofur-resistant E. coli isolates were obtained from 96 healthy pigs. The antibiotic resistance profile was tested, and all 27 isolates were classified as multidrug-resistant (MDR). A high proportion of isolates were resistant to cephalosporins, ampicillin, ciprofloxacin, and tetracyclines. The ESBL production was observed in 85% of isolates by double-disc synergy test. The MDR- E. coli isolates harbored ESBL genes, such as bla
TEM , blaCTX-M-1 , blaCTX-M-2 , and blaCTX-M-8,25. In addition, other antibiotics resistance genes (ARGs) were also detected, such as sul2, ant(3″)-I, tetA , and mcr-1. The mobilization of the blaCTX-M gene was confirmed for nine E. coli isolates by conjugation assays. The presence of blaCTX-M on mobile genetic elements in these isolates was demonstrated by Southern blot hybridization, and the resistance to cephalosporins was confirmed in the transconjugants. Our results indicate the prevalence of CTX-M-producing E. coli strains harboring mobile genetic elements in the normal microbiota of healthy pigs. Conclusions These findings highlight the significance of ESBL genes as a global health concern in livestock and the potential spread of antimicrobial resistance to other members of the gastrointestinal tract microbiota. [ABSTRACT FROM AUTHOR]- Published
- 2024
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