1. Resveratrol mediates mitochondrial function through the sirtuin 3 pathway to improve abnormal metabolic remodeling in atrial fibrillation.
- Author
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Cao Y, Cui L, Tuo S, Liu H, and Cui S
- Subjects
- Animals, Mice, Cell Line, Signal Transduction drug effects, Membrane Potential, Mitochondrial drug effects, Mitochondria metabolism, Mitochondria drug effects, Mitochondria, Heart metabolism, Mitochondria, Heart drug effects, Reactive Oxygen Species metabolism, Resveratrol pharmacology, Sirtuin 3 metabolism, Atrial Fibrillation metabolism, Atrial Fibrillation drug therapy
- Abstract
This study investigated the impact of resveratrol on abnormal metabolic remodeling in atrial fibrillation (AF) and explored potential molecular mechanisms. An AF cell model was established by high-frequency electrical stimulation of HL-1 atrial muscle cells. Resveratrol concentrations were optimized using CCK-8 and flow cytometry. AF-induced increases in ROS and mitochondrial calcium, along with decreased adenosine triphosphate (ATP) and mitochondrial membrane potential, were observed. Resveratrol mitigated these changes and maintained normal mitochondrial morphology. Moreover, resveratrol acted through the SIRT3-dependent pathway, as evidenced by its ability to suppress AF-induced acetylation of key metabolic enzymes. SIRT3 overexpression controls acetylation modifications, suggesting its regulatory role. In conclusion, resveratrol's SIRT3-dependent pathway intervenes in AF-induced mitochondrial dysfunction, presenting a potential therapeutic avenue for AF-related metabolic disorders. This study sheds light on the role of resveratrol in mitigating AF-induced mitochondrial remodeling and highlights its potential as a novel treatment for AF.
- Published
- 2024
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