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Your search keyword '"Saidijam, Massoud"' showing total 10 results
Start Over Author "Saidijam, Massoud" Publisher pasteur institute of iran
10 results on '"Saidijam, Massoud"'

2. Expression Analysis of mir-21 and mir-221 in Cancerous Tissues from Iranian Patients with Gastric Cancer

Author

Effatpanah, Hosein, Yadegarazari, Reza, Karami, Manoochehr, Majlesi, Amir, Shabab, Nooshin, and Saidijam, Massoud

Subjects
Male, Gene Expression Profiling, Stomach neoplasms, Gene Expression, Iran, Middle Aged, Real-Time Polymerase Chain Reaction, MicroRNAs, ROC Curve, Tumor markers, Area Under Curve, Biomarkers, Tumor, Humans, Original Article, Female, Early Detection of Cancer
Abstract

Background: Early detection is a key to survival for gastric cancer. Molecular markers such as miRNA (microRNA) can have great importance in the early diagnosis of gastric cancer. Expression of miR-21 and miR-221 are deregulated in many types of human cancers. This study aimed to investigate the differences in miRNA expression patterns within the Iranian population. Methods: Total RNA was extracted from gastric cancer tissues and adjacent non-cancerous tissues from 32 patients. Expression levels of miR-21 and miR-221 were detected by Real time RT-PCR using a specific primer, with 5s rRNA as the internal reference gene. Results: Our data showed that the expression levels of miR-21 and miR-221 in gastric cancer samples were significantly higher than in paired non-cancerous samples (P < 0.05). The receiver operating characteristic (ROC) analyses yielded the area under the curve (AUC) values of 80.30 for miR-21 and 93.30 for miR-221, and combined ROC analysis revealed the highest AUC value of 96.90 in discriminating GC patients from healthy controls. Conclusion: It seems that miR-21 and miR-221 expression pattern in Iranian patients with gastric cancer are similar to any other population. Considering the increased expression level of two miRNAs in cancerous tissue compared to normal tissue as well as the area under ROC curve, miR-21 and miR-221 can be used for early detection of gastric cancer.

Published
2015

10. Semi-quantitative analysis of HOXA11, leukemia inhibitory factor and basic transcriptional element binding protein 1 mRNA expression in the mid-secretory endometrium of patients with endometriosis.

Author

Alizadeh Z, Shokrzadeh N, Saidijam M, and Sanoee MF

Subjects
Base Sequence, Case-Control Studies, DNA Primers, Electrophoresis, Agar Gel, Female, Humans, Reverse Transcriptase Polymerase Chain Reaction, Endometriosis genetics, Endometrium metabolism, Homeodomain Proteins genetics, Kruppel-Like Transcription Factors genetics, Leukemia Inhibitory Factor genetics, RNA, Messenger genetics
Abstract

Background: HOXA11 and leukemia inhibitory factor (LIF) and basic transcriptional element binding protein1 (BTEB1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. In this case-control study, the expression pattern of these mRNA was evaluated in patients with endometriosis at the time of implantation. We also describe a semi-quantitative RT-PCR protocol optimized in our laboratory., Methods: Eight patients with endometriosis were considered as our case and 8 fertile women as control group. Expression levels of HOXA11, LIF and BTEB1 mRNA were measured in endometrium during the mid-secretory phase using semi-quantitative RT-PCR., Results: We describe the detailed procedure for the analysis of HOXA11, LIF and BTEB1 mRNA levels. Endometrial HOXA11 and LIF mRNA expression levels (normalized to beta-actin expression) were significantly decreased in endometrium of infertile patients with endometriosis compared with healthy fertile controls at the time of implantation (P < 0.05). A similar trend was seen in BTEB1 mRNA expression., Conclusion: The results suggest that the alteration in expression pattern of the some genes could account for some aspects of infertility in endometriosis.

Published
2011

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