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Dissolved organic matter (DOM) plays a pivotal role in drinking water treatment, influencing the performance of unit processes and final water quality (e.g. disinfection byproduct risk). Biofiltration is an effective method of reducing DOM, but currently lacks a comprehensive appreciation of the association between microbial profiles and biofiltration performance. In this study, bench-scale biofiltration units inoculated with microbial consortia from river and soil matrices were operated successively for comparing their efficacy in terms of DOM removal. The results showed that biofiltration units receiving soil microbes were significantly superior (p < 0.05) to those receiving river inoculated microbes in terms of decomposing DOM recalcitrant fractions and reducing DBP formation potential, resulting in DOC and DBP precursor removals of up to 58.4 % and 87.9 %, respectively. Characterization of the taxonomic composition revealed that differences in the microbial assembly of the two biofilter groups were subject to deterministic rather than stochastic factors. Furthermore, more complicated interspecific relationships and niche structures in soil inoculated biofilters were deciphered by co-occurrence network, providing a plausible profile on a taxonomic division of labor in DOM stepwise degradation. Accordingly, the contribution of microbial compositions was found to be of greater importance than the GAC mass and biomass attached to the media. Thus, this study has advanced the understanding of microbial-mediated DOM decomposition in biofiltration, and also provided a promising strategy for enhancing the process for water use via developing appropriate engineered consortia of bacteria., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Biological activated carbon filter (BAC) is one of the most effective technologies for removing disinfection by-product (DBP) precursors from water. Biochar is a lower-cost medium that has the potential to replace granular activated carbon in BAC applications, thus leading to the development of biological biochar filter (BCF). This study compared BCF with BAC for the removal of DBP precursors using column experiments. Both BCF and BAC achieved the removal of DBP precursors, resulting in concentrations of all DBP formation potential below the World Health Organization guideline values for drinking water. Bromodichloromethane and unknown DBP precursor removal by BCF was comparable to that by BAC. However, BAC removed more chloroform and dichloroacetontrile precursors than BCF. For microbial community analysis, cell numbers in a bottom layer (inlet) of BCF and BAC columns were higher than those in the top layer. The abundances of Nordella and a microbial genus from Burkholderiaceae at the bottom layer showed a strong correlation to the number of DBP precursors removed and were comparable in BCF and BAC. This finding likely contributes to the similarities between DBPs species removed and the removal performances of some known and unknown DBP precursors by BCF and BAC. Overall results from this study revealed that biochar can be served as a low-cost and sustainable replacement of activated carbon in water filter for DBP precursor removal., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Biofiltration in drinking water treatment (BDWT) are popular as it holds promise as an alternative to chemical treatments, yet our understanding of the key drivers and trends underlying bacterial evolution within this process remains limited. While plasmids and phages are recognized as the main vectors of horizontal gene transfer (HGT), their roles in shaping bacterial evolution in BDWT remain largely unknown. Here we leverage global metagenomic data to unravel the primary forces driving bacterial evolution in BDWT. Our results revealed that the primary vector of HGT varies depending on the type of source water (groundwater and surface water). Both plasmids and phages accelerated bacterial evolution in BDWT by enhancing genetic diversity within species, but they drove contrasting evolutionary trends in functional redundancy in different source water types. Specifically, trends towards and away from functional redundancy (indicated as gene-protein ratio) were observed in surface-water and groundwater biofilters, respectively. Virulent phages drove bacterial evolution through synergistic interactions with bacterial species capable of natural transformation and with certain natural compounds that disrupt bacterial cytoplasmic membranes. Genes relating to water purification (such as Mn(II)-oxidizing genes), microbial risks (antibiotic resistance genes), and chemical risk (polycyclic aromatic hydrocarbons) were enriched via HGT in BDWT, highlighting the necessity for heighted focus on these useful and risky objects. Overall, these discoveries enhance our understanding of bacterial evolution in BDWT and have implications for the optimization of water treatment strategies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

This study explored the potential of sand biofiltration for tertiary treatment of real refinery wastewater. The biofilter (2 cm (I.D.) x 15 cm (L)) operated on secondary treated refinery wastewater at flow rate of 1 mL/min had empty bed contact time (EBCT) of 47.12 min for one circulation. Maximum reduction in COD after 4, 8 and 12 times recirculation was 25 %, 52 % and 56 %; while the TOC reduction was 33 %, 43 % and 51 %, respectively, after biofilm development over 30 days. Quantification using two dimensional gas chromatography - time of flight mass spectrometry (GCxGC-TOF MS) revealed that several of the identified target compounds could not be detected in the wastewater after 12 recirculations. After 8 times recirculation, most of the compounds showed very high removal efficiency. For biofiltration over the flow rate range 2-10 mL/min, the reduction in COD and NH 4 + -N ranged from 62-73 % and 78-86 %, respectively, after 8 times recirculation. The nitrite concentration first increased and subsequently decreased, while the nitrate concentration continuously increased with increase in the number of recirculations. Solid phase micro-extraction (SPME) analysis of the aqueous phase using GCxGC-TOF MS and a semi-quantitative approach indicated that the removal of predominant classes of compounds was greater than 95 % after 8 times recirculation, with maximum reduction occurring in the first pass through the biofilter. Assimilable organic carbon (AOC) reduction was 98 % after 8 times recirculation. Metagenomic analysis revealed that Proteobacteria was the most dominant phylum in the biofilter. Many known polynuclear aromatic hydrocarbon (PAH) degraders, such as Sphingomonadales, Burkholderiales, Rhodobacterales and Rhodospirillales, were found in the biofilter leading to high removal efficiency of hazardous organic pollutants., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Nature-based solutions are encouraged for treating oilfield produced water from oil and gas extraction, a crucial undertaking that aligns with the Canadian oil sands industry's ambitious goal of zero waste, and the globally recognized Sustainable Development Goals (SDGs) pertaining to water conservation and ecosystem preservation. This study explored the use of peat-mineral mix (PMM), a leftover of inevitable oil sands mining, for treating low and high-strength wastewaters during biofiltration, which contained large molecular weight (44.3 kDa), which include alcohols, aliphatics, aromatics, and ketones, and can impart high toxicity to both fauna and flora (MicroTox: 99 %). The breakthrough curve indicated an effective initial adsorption phase driven by advection within the column dynamics. For complete organics removal and mechanistic insights, the wastewater was re-circulated in a continuous mode for up to 42 days. Here, we found that chemical oxygen demand was reduced from ∼85,000 mg/L to ∼965 mg/L). Kinetics investigations along with physicochemical characterization of PMM and wastewater suggested that chemisorption and anaerobic digestion contributed to the overall removal of contaminants. Chemisorption, led by hydrogen bonding and hydrophobic interactions, was the dominant mechanism, with a limited contribution from physical adsorption (surface area: 2.85 m2/g). The microbial community within the PMM bed was rich/diverse (Shannon > 6.0; Chao1 > 600), with ∼ 50 % unclassified phylotypes representing 'microbial dark matter'. High electric conductivity (332.1 μS cm -1 ) of PMM and the presence of Geobacter, syntrophs, and Methanosaeta suggest that direct interspecies electron transfer was likely occurring during anaerobic digestion. Both low and high-strength wastewaters showed effective removal of dissolved organics (e.g., naphthenic acids, acid extractable fraction, oil and grease content), nutrients, and potentially toxic metals. The successful use of PMM in treating oilfield produced water offers promising avenues for embracing nature-based remediation solutions at oil refining sites., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Biofiltration is an environmentally 'green' technology that is compatible with the recently proposed sustainable development goals, and which has an increasingly important future in the field of water treatment. Here, we explored the impacts of bioelectrochemical integration on a bench-scale slow rate biofiltration system regarding its performance in reclaimed water treatment. Results showed that the short-term (<3 months) integration improved the removal of natural organic matter (NOM) (approximately 8.8%). After long-term (5 months and thereafter) integration, the cathodic charge transfer resistance was found to have a significant reduction from 2662 to 1350 Ω. Meanwhile, bioelectrochemical autotrophic sulfate (SO 4 2- ) reduction (over 27.6% reduction) through the syntrophic metabolism between hydrogen oxidation strains (genus Hydrogenophaga) and sulfate-reducing microbes (genera Dethiobacter, Desulfovibrio, and Desulfomicrobium) at the cathodic region was observed. More significantly, the microbial-derived chromophoric humic substances were found to act as electron shuttles at the cathodic region, which might facilitate the process of bioelectrochemical SO 4 2- reduction. Overall, this study provided valuable insights into the potential application of bioelectrochemical-integrated biofilter for simultaneous reduction of NOM and SO 4 2- treating reclaimed water., Competing Interests: Declaration of competing interest The authors declare that they have no known competing for financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Biofiltration is used worldwide to provide safe potable water due to its low energy demand and excellent treatment performance. For instance, in Denmark, over 95% of drinking water is supplied through groundwater-fed rapid sand filters (RSF). Bacteriophages, viruses that infect bacteria, have been shown to shape the taxonomic and functional composition of microbial communities across a range of natural and engineering systems. However, phages in the biofiltration systems are rarely studied, despite the central role microbes play in water purification. To probe this, metagenomic data from surface water, groundwater and mixed source water biofiltration units (n = 26 from China, Europe and USA) for drinking water production were analysed to characterize prokaryotic viruses and to identify their potential microbial hosts. The source water type and geographical location are found to exert influence on the composition of the phageome in biofilters. Although the viral abundance (71,676 ± 17,841 RPKM) in biofilters is only 14.4% and 17.0% lower than those of the nutrient-rich wastewater treatment plants and fresh surface waters, the richness (1,441 ± 1,046) and diversity (Inverse Simpson: 91 ± 61) in biofiltration units are significantly less by a factor of 2-5 and 3-4, respectively. In depth analysis of data from 24 groundwater-fed RSFs in Denmark revealed a core phageome shared by most RSFs, which was consistently linked to dominant microbial hosts involved in key biological reactions for water purification. Finally, the high number of specific links detected between phages and bacterial species and the large proportion of lytic phages (77%) led to the conjecture that phages regulate bacterial populations through predation, preventing the proliferation of dominant species and contributing to the established functional redundancy among the dominant microbial groups. In conclusion, bacteriophages are likely to play a significant role in water treatment within biofilters, particularly through interactions with key bacterial species., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper, (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The health risks associated with manganese (Mn) in drinking water, and an improved understanding of Mn accumulation within, and subsequent release from, distribution systems, have increased the need for robust, sustainable treatment options to minimize Mn concentrations in finished water. Biofiltration is an established and effective method to remove Mn in groundwater however, Mn removal in surface water biofilters is an emerging treatment process that has not been extensively studied. Seasonal variations in water temperature can present an operational challenge for surface water biofilters which may see reduced Mn removal under colder conditions. This study examined the microbiomes of surface water biofilters at three utilities (ACWD WTP, WTP B, and WTP D) which all experienced similar seasonal fluctuations in influent water temperature. High Mn removal was observed at the ACWD WTP for much of the year, but Mn removal decreased with a concurrent decrease in the influent water temperature (58% ± 22%). In contrast, both WTP B and WTP D achieved year-round Mn removal (84% ± 5% and 93% ± 8% respectively). Marker gene (16S rRNA) sequencing analysis of the biofilter microbiomes identified a high abundance of Betaproteobacteria in WTP B and WTP D (37% ± 12% and 21% ± 3% respectively), but a low abundance of Betaproteobacteria in the ACWD WTP (2% ± 2%). The microbiomes of new bench-scale biofilters, in operation at the ACWD WTP, were also investigated. The abundance of Betaproteobacteria was significantly greater (p < 0.05) after the biofilters had acclimated than before acclimation, and differential abundance analysis identified 6 genera within the Betaproteobacteria class were enriched in the acclimated microbiome. Additionally, the acclimated biofilters were able to maintain high Mn removal performance (87% ± 10%) when the influent water temperature decreased to 10 °C or less. Further analysis of previously published studies found the abundance of Betaproteobacteria was also significantly greater (p < 0.001) in biofilters with sustained Mn removal than in biofilters which did not treat for Mn as a contaminant, despite differences in design scale, source water, and media type. Microbiome network analysis identified multiple co-occurrence relationships between Betaproteobacteria and Mn oxidizing bacteria in the WTP B and WTP D biofilters, suggesting indirect contributions by Betaproteobacteria to biological Mn oxidation. These co-occurrence relationships were not present in the full-scale ACWD WTP microbiome. Whether the role of Betaproteobacteria in biological Mn oxidation is direct, indirect, or a combination of both, they are consistently present at a high abundance in both groundwater and surface water biofilters with sustained Mn removal, and their absence may contribute to the seasonal fluctuations in Mn removal observed at the ACWD WTP. This new insight to Betaproteobacteria and their role in Mn biofiltration could contribute to water innovation and design that would improve the reliability of Mn removal., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

1,4-Dioxane is a drinking water contaminant of emerging concern. Because conventional and many advanced drinking water treatment technologies are ineffective for 1,4-dioxane removal, cost-effective technologies for the removal of 1,4-dioxane at drinking water-relevant concentrations are needed. In this research, a gravity-fed, cometabolic biofiltration system was developed to degrade 1,4-dioxane that was spiked into coagulated, settled surface water at a concentration of ∼10 µg/L. Objectives were to determine whether cometabolic degradation of trace levels of 1,4-dioxane can be sustained using n-butane as primary substrate and whether filter media properties and empty bed contact time (EBCT) affect biofiltration efficiency. A mixed culture of bacteria derived from the Cape Fear River basin and previously enriched using isobutane served as inoculum for biologically active filters. Two granular activated carbons (GACs) with different grain sizes and one carbonaceous resin were used as attachment media, and n-butane served as the primary substrate for biologically active filters. Non-inoculated controls with the same media were evaluated in parallel to distinguish between biological and adsorptive removals of 1,4-dioxane. For the duration of the pilot study (>3 months), 1,4-dioxane was degraded in inoculated biofilters receiving n-butane. In control filters containing larger and smaller grain GAC, 1,4-dioxane broke through completely within 750 and 1250 bed volumes, respectively, corresponding to 15 to 30 days of operation at an EBCT of 30 min. 1,4-Dioxane removal increased with increasing EBCT in all biologically active filters. At an EBCT of 30 min, the biologically active GAC filter containing the larger-grain GAC removed on average 87% of 1,4-dioxane at pseudo steady-state. When the hydraulic loading rate was decreased to achieve an overall EBCT of 60 min, 1,4-dioxane was removed to <1 µg/L in the biologically active GAC filter containing the larger-grain GAC. Activity-based labeling showed the presence of catalytically active monooxygenases in backwash water from biologically active filters that degraded 1,4-dioxane. Amplicon sequencing results showed that while taxa shifted after the initial inoculation of biologically active filters, taxa in biologically active filters remained more similar to the inoculum than those in the non-inoculated control filters. Overall, results of this research demonstrate that cometabolic degradation of 1,4-dioxane at trace levels is possible for extended periods of time in inoculated biofilters that receive n-butane as primary substrate., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Stormwater biofilters have demonstrated promising yet variable removal of faecal microorganisms. Significant antimicrobial-producing plants have been proposed as an inexpensive, safe and easily adaptable component of biofilter design to enhance faecal pathogen treatment. The aim of the present study was to investigate whether significant antimicrobial-producing plants improved faecal bacterial inactivation throughout the biofilter soil profile, focusing on four key treatment zones. These were specifically the top sediment/surface layer; the rhizosphere (soil directly attached to and influenced by plant roots); bulk soil (soil not directly associated with roots); and the submerged/saturated zone. Biofilters were configured with either: (1) no plant; (2) Carex appressa, the most highly recommended plant species in Australian biofilter adoption guidelines; or (3) one of two significant antimicrobial-producing Australian plant species, Melaleuca linariifolia or Melaleuca fulgens (n = 3 each). Following 16 months' maturation, systems were dosed with semi-synthetic stormwater containing faecal bacteria Escherichia coli and Enterococcus faecalis to monitor their ensuing die-off within all major biofilter treatment zones. Bacterial inactivation was generally more rapid in M. fulgens and M. linariifolia than C. appressa biofilters, with E. faecalis demonstrating an overall enhanced resistance to inactivation. Top sediment tended to exhibit the highest inactivation rates, significantly correlated with sunlight exposure. Conversely, the rhizosphere supported comparatively prolonged faecal bacterial survival. The authors recommend further investigation of melaleucas and similar highly antimicrobial-producing plants for enhanced faecal pathogen treatment within biofilters and related treatment contexts., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

For the potable reuse of municipal wastewater effluent, carbon based advanced treatment (CBAT) using coagulation, ozonation, biofiltration and/or granular activated carbon (GAC) adsorption is a promising approach for controlling disinfection byproduct (DBP) formation. However, CBAT can also favor a shift in DBP formation to more toxic brominated DBP species. To protect public health, treatment-specific DBP formation and speciation trends need to be identified and understood. First, this study systematically evaluated the treatment of six wastewater effluents with four CBAT process trains (experimental n was 55) and measured DBP formation and speciation trends. Overall, CBAT decreased DBP formation by >90% and GAC preferentially removed highly-reactive effluent organic matter as indicated by lower yields of both highly-forming and highly-toxic classes of carbonaceous and nitrogenous DBPs. Since GAC treatment also induced systematic speciation changes by increasing the ratio of bromide to dissolved organic matter, the second part of this study focused on understanding the health impacts of DBP speciation changes on calculated additive toxicity (CAT). Based on the evaluation of 20 DBPs, measured using established methods, the CAT values from cyto- and genotoxicity metrics decreased by as much as 85% due to high levels of precursor removal by GAC. Expanding the evaluation to include 52 DBPs, measured using more extensive analytical methods, resulted in the same conclusions. This study also developed a "speciation potency" metric, that re-scales class-by-class speciation trends using toxic potency factors (e.g., cytotoxicity [LC 50 ]). The observed shifts in DBP speciation after treatment increased the class-level toxic potency factors by up to a factor of 4; a greater amount of precursor removal is required for treatment to reduce toxicity, which was achieved with CBAT trains. This proposed approach of combining speciation potency with DBP yields enables evaluation of DBP-associated risk with easily measured surrogates (i.e., bromide and dissolved organic carbon [DOC]). By identifying and quantitatively comparing DBP formation and speciation trends over multiple wastewater effluents and treatment trains, this study demonstrates that CBAT can be a robust approach to DBP precursor removal for potable reuse., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

A new focus on biofiltration has emerged that aims to shape microbial communities to improve treatment efficacy. It is therefore necessary to understand the linkages between microbial community structure and biofilter function. However, the assembly and interaction of microbial communities in biological activated carbon (BAC) filters are unknown. In this study, we selected one coal-based granular activated carbon (GAC), GAC-13, with simultaneously developed micropore and micro-level macropore volume used for a bench-scale BAC column experiment, and compared it with other coal-based GACs and wood-based GAC in terms of the dissolved organic carbon (DOC) removal and microbial community characteristics. The results showed that there was no difference between the DOC removal efficiency of BAC-13 and the other two coal-based BAC filters with high iodine value in the period dominated by adsorption, while the DOC removal efficiency of BAC-13 (64.7±0.6%) was significantly higher than that of other BAC filters (36.3±0.8-54.1±0.4%) with a difference of 0.3-0.7 mg/L in DOC during the steady state. The bacterial communities were strongly assembled by deterministic rather than stochastic factors, where the surface polarity of GAC had a greater effect on the microbial communities than its physical properties. The corresponding co-occurrence network revealed that microbes in the BAC filter may be more cooperative than competitive. The keystone bacterium Hyphomicrobium, which had a relatively low abundance, contributed 0.3-1% more to the most abundant functions and produced 5-21 proteins/(g·GAC) more than the dominant bacterium Sphingobium. The metaproteomic-based approach could provide more accurate information regarding the contributions of different species to metabolic functions. The pore size distribution of GAC was found to be an important factor in determining BAC filter performance; the most important pore sizes were micropores and micro-level macropores (0.2-10 μm and >100 μm in diameter), and the latter impacted the abundance of keystone species. Overall, our findings provide new insights into shaping microbial communities by optimizing pore size structure to improve BAC performance, especially the abundance of keystone species., (Copyright © 2022. Published by Elsevier Ltd.)

This study investigated treatment strategies which accelerated the acclimation of new Mn-removing biofilters to help utilities respond to changing Mn regulations, such as the recent introduction of a health-based maximum acceptable concentration and a reduction in the aesthetic objective for Mn in drinking water by Health Canada. Bench-scale filters of either GAC or anthracite media were fed with applied water containing Mn (17-61 μg/L) from a full-scale plant over 294 days. Treatment strategies included the addition of H 2 O 2 (1 mg/L) and/or an increase in pH from 6.8 to 7.5 through the addition of NaOH. The potential physico-chemical and biological mechanisms responsible for accelerated biofilter acclimation under the various redox conditions were investigated through thermodynamic modelling, to predict homogeneous Mn oxide formation, and 16S rRNA gene amplicon sequencing, to characterize the microbial community within the filters. GAC filters treated with NaOH, and both H 2 O 2 and NaOH, were the first to acclimate (< 20 μg/L Mn in filter effluent) after 59 and 63 days respectively, while the ambient GAC filter took almost 3 times as long to acclimate (168 days), and the anthracite filters which received the same chemically adjusted water took almost 4 times as long (226 and 251 days, respectively). The accelerated acclimation in the treated GAC filters was likely due to physico-chemical oxidation via three potential mechanisms: (1) homogeneous oxidation of dissolved Mn(II) to Mn(III)/Mn(IV) oxides and the subsequent removal of oxides from solution through adherence to the GAC surface, (2) adsorption of dissolved Mn(II) to GAC and subsequent homogeneous or biological oxidation, or (3) formation of colloidal Mn(III)/Mn(IV) oxides and subsequent adsorption of dissolved Mn(II) to the Mn colloids. In the untreated GAC filter and all anthracite filters, which did not benefit from improved redox conditions or an active surface, physico-chemical mechanisms alone were insufficient for consistent Mn removal to less than 20 μg/L. Acclimation in these filters was delayed until a microbiome enriched with bacteria capable of biological nitrification and Mn oxidation evolved within the filters. The acclimated microbiome was consistent between GAC and anthracite filters and was significantly different from the non-acclimated microbiome (p < 0.001) initially formed during the early operation of the filters. Interestingly, treatment with NaOH, and NaOH and H 2 O 2 , which accelerated physico-chemical oxidation in GAC filters, was observed to delay the development of biological oxidation in anthracite filters, and thus deferred acclimation. Although some filters took longer to acclimate than others, once acclimation was reached all filters had a similar microbiome and were able to consistently remove Mn to below 20 µg/L., (Copyright © 2021. Published by Elsevier Ltd.)

Membrane fouling by influent biopolymers, and the formation of surface biofilms, are major obstacles to the practical application of membrane technologies. Identifying reliable and sustainable pre-treatment methods for membrane filtration remains a considerable challenge and is the subject of continuing research study worldwide. Herein, the performance of a bench-scale gravity-driven up-flow slow biofilter (GUSB) as the pre-treatment for ultrafiltration to reduce membrane fouling is presented. Dissolved organic carbon (DOC) was shown efficiently removed by the GUSB (around 80%) when treating a natural water influent. More significantly, biopolymers, with molecular weight (MW) between 20 kDa and 100 kDa, were effectively removed (62.8% reduction) and this led to a lower rate of transmembrane pressure (TMP) development by the UF membrane. Microbial diversity analysis further unraveled the function of GUSB in shaping microbes to degrade biopolymers, contributing to lower accumulation and different distribution pattern of SMP on the membrane surface. Moreover, the biofilm formed on the membrane surface after the pre-treatment of GUSB was observed to have a relative porous structure compared to the control system, which can also affect the fouling development. Long-term operation of GUSB further revealed its robust performance in reducing both natural organic matters and UF fouling propensity. This study overall has demonstrated the potential advantages of applying a GUSB to enhance UF process performance by reducing biofouling and improving effluent quality., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing for financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

The dark false mussel Mytilopsis leucophaeata occurs as non-native species in Asia, Europe and South America. Despite the low population density usually found in its native range, M. leucophaeata forms dense clusters in newly invaded areas which lead to impacts on local organisms and environment. Some of the impacts of non-native species on newly colonized areas may be positive. However, despite the empirical evidences of increase in water transparency by freshwater dresseinids, the hypothesis that dark false mussel enhances the quality of brackish water has never been tested so far. Thus, the present study aimed to determine M. leucophaeata impacts on the water quality of a nutrient-enriched coastal lagoon by (1) analyzing a historical water dataset for pre- and post-invasion at Rodrigo de Freitas Lagoon (Brazil) and (2) experimentally testing dark false mussel direct effects on water quality. Historical field data evidenced significant lower phytoplankton abundance and chlorophyll a, and higher water transparency for the post-invasion period. These pattens were also supported by time-series analyses, which detected a decreasing trend for total coliforms density and an increased trend for dissolved oxygen over time. Moreover, time series indicated a gradual increase in lagoon water level with time, but none or minor changes were evidenced for floodgates operation routine, meteorological variables, and the frequency of sewage input. In microcosms, M. leucophaeata was effective to increase water transparency and decrease E. coli densities. The combination of field and laboratory data partially supported the hypothesis that M. leucophaeata invasion promoted an improvement in lagoon water quality, but increased phosphorus availability through excretion in microcosm assays. Management of dark false mussel populations seems to be a potential strategy for water quality improvement within already invaded systems where high sewage-enriched effluents are released. However, as non-native species often unbalance ecological relationships and functioning of the invaded ecosystems, new introductions of M. leucophaeata must be avoided., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Antibiotic resistance genes (ARGs) are being detected in drinking water frequently, constituting a major public health issue. As a typical drinking water treatment process, the biofilter may harbour various ARGs due to the filter biofilms established during the filtration process. The objective of this study was to investigate the behaviour of ARGs (bla CTX-M , bla OXA-1 , bla TEM , ermB, tetA, tetG, tetQ, tetW, tetX, sul 1, sul 2, dfrA1 and dfrA12) and their possible association with bacteria in a bench-scale biofiltration system. The impact of filter media on horizontal gene transfer (HGT) was also explored using a model conjugative plasmid, RP1. The biofiltration system comprised four types of biofilters, including sand, granular activated carbon (GAC), GAC sandwich, and anthracite-sand biofilters. Results showed that although the absolute abundance of ARGs decreased (0.97-log reduction on average), the ARGs' abundance normalised to bacterial numbers showed an increasing trend in the filtered water. Biofilms collected from the surface layer revealed the lowest relative abundance of ARGs (p < 0.01) compared to the deeper layer biofilms, indicating that the proportion of ARG-carrying bacteria was greater in the lower position. Most chosen ARG numbers correlated to Proteobacteria, Acidobacteria and Nitrospirae phyla, which accounted for 51.9%, 5.2% and 2.0% of the biofilm communities, respectively. GAC media revealed the highest transfer frequency (2.60 × 10 -5 ), followed by anthracite (5.31 × 10 -6 ) and sand (2.47 × 10 -6 ). Backwashing can reduce the transferability of RP1 plasmid significantly in biofilms but introduces more transconjugants into the planktonic phase. Overall, the results of this study could enhance our understanding of the prevalence of ARGs in drinking water biofiltration treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

By applying favorable oxic and oligotrophic conditions through subsequent aeration and an additional infiltration step, the sequential managed aquifer recharge technology (SMART) was proven to better remove trace organic chemicals (TOrCs) than conventional MAR systems. To minimize the physical footprint, pumping costs and hydraulic retention times, as well as to overcome limitations of site-specific heterogeneities of such systems, the SMART concept was further upgraded by two main engineered technologies. This SMARTplus bioreactor is comprised of an infiltration trench and highly homogenous porous media to provide high infiltration rates and plug-flow conditions. Additionally, an in-situ oxygen delivery device, in particular a self-designed PDMS gas-liquid membrane contactor, was designed to establish favorable subsurface oxic conditions. This novel SMARTplus technology was investigated at pilot scale and is designed for advanced water treatment either in the context of water reuse or treatment of impaired surface water. To determine the design specifications and to construct a pilot-scale membrane contactor, the mass transfer coefficients of the PDMS membrane were investigated at lab-scale for varying Reynold numbers (0.2-2). With the help of the customized membrane contactor, homogenous, bubble-free and passive oxygen delivery could be successfully demonstrated at pilot-scale under laminar flow conditions and short contact times. Oxygen concentrations downstream of the membrane contactors met the design specifications (>1 mg/L) as long as the required feed water quality was provided. However, high NH 4 + concentrations in the secondary effluent resulted in higher and unsteady oxygen demand than the target oxygen transfer rates could meet and suboxic conditions prevailed. Although a 20-50% enhancement in the removal of certain compounds (4-FAA, antipyrine, sulfamethoxazole, and citalopram) was achieved, demonstration of the full potential of enhanced TOrC removal by SMARTplus was hindered due to unsteady feed water quality., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Recovering and reusing treated wastewater effluent is a sustainable and cost-effective practice for addressing global water sustainability. To date, most potable reuse advanced water treatment (AWT) solutions are based on reverse osmosis (RO), which generates a continuous reject stream of concentrated brine waste. Ozone-biofiltration based solutions have been investigated as a potential alternative for RO. However, implementation of ozone-biofiltration for potable reuse projects around the world has been limited. The goal of this study was to conduct an extended field investigation of ozone-biofiltration treatment to address regulatory, design, and operational hurdles that may hinder implementation in water-short areas. For 16 months, two parallel biological activated carbon (BAC) filters were operated at empty bed contact times (EBCTs) of 10 min and 20 min treating up to 60,000 and 30,000 bed volumes (BVs), respectively, of sand filtered effluent from a municipal wastewater treatment process. BAC 1 (EBCT = 10 min) and BAC 2 (EBCT = 20 min) used Calgon Filtrasorb 400 granular activated carbon (GAC) as filter media, with equal bed depths of 0.8 m. Increasing the specific ozone dose from 0.9 to 2.0 provided a muted response with respect to oxidation of contaminants of emerging concern (CECs) that are resistive to ozonation. N-Nitrosodimethylamine (NDMA) was generated during ozonation, with the average concentration of NDMA in ozonated effluent being 40.4 ng/L. In BAC 1 (EBCT = 10 min), NDMA was fully removed during the first month of study (<2000 BVs), partially removed between 2000 and 20,000 BVs, and completely removed when monitored between 57,000 and 62,000 BVs. These trends clearly reveal time-dependent interactions between carbon-based (e.g., adsorption) and non-carbon-based (e.g., biodegradation) removal mechanisms. In BAC 2 (EBCT = 20 min), almost all CECs, excluding NDMA, were removed consistently throughout the study (through ∼30,000 BVs). This indicates a somewhat different interaction between carbon-based and non-carbon-based removal in the more lightly loaded BAC 2, compared to BAC 1. After 482 days of operation, BAC 1 (EBCT = 10 min) produced effluent with lower NDMA concentration (<2 ng/L) than BAC 2 (10 ng/L), confirming prior evidence of cometabolic NDMA biodegradation pathways operable in more heavily loaded BACs. These findings emphasize the need for extended field testing (50,000 BVs or greater). BAC 1 removed TOC in effluent until it plateaued at around 6 mg/L after 60,000 BVs, whereas BAC 2 effluent plateaued at around 4 mg/L. Under plateau conditions, BAC 1 and BAC 2 with sand filter pretreatment and ozonation appear to have a gross TOC removal potential of around 0.2-0.3 kg of TOC removed per day per cubic meter of carbon media (kg/d/m 3 ). A comparative analysis of findings from this study and results from a past ozone-BAC study in the Reno area (termed BAC 3 operated downstream of membrane filter with an EBCT of 30 min) shows that higher TOC removal was observed in BAC with shorter EBCT and upstream sand filter compared to BAC with longer EBCT and upstream membrane filter. The present study addresses the regulatory and financial concerns associated with ozone-BAC performance in potable reuse applications. Improved comprehension of ozone-BAC performance, coupled with its reduced capital and operations and maintenance (O&M) costs compared to RO, may accelerate the full-scale implementation of ozone-BAC treatment as a sustainable solution for the rapidly emerging potable reuse market., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Rapid sand biofiltration (RSBF) is widely used for the removal of contaminants from drinking water treatment systems. Biofilm microbiomes in the biofilter media play essential roles in biotransformation of contaminants, but is not comprehensively understood. This study reports on Mn(II) oxidation and the core microbiomes in magnetite sand RSBF (MagS-RSBF) and manganese sand RSBF (MnS-RSBF). MnS-RSBF showed a relatively higher Mn(II) removal rate (40-91.2%) than MagS-RSBF during the start-up. MagS-RSBF and MnS-RSBF had similar Mn(II) removal rates (94.13% and 99.16%) over stable operation for 80 days. Mn(II) removal rates at different depths in the MnS-RSBF reactor significantly changed with operation time, and the filter in the upper layer of MnS-RSBF made the largest contribution to Mn(II) oxidation once operation had stabilized. PacBio single molecule sequencing of full-length 16S rRNA gene indicated that biofilter medium had a significant impact on the core microbiomes of the biofilms from the two biofilters. The magnetite sand biofilter facilitated the enrichment of Mn(II)-oxidizing biofilms. The dominant populations consisted of Pedomicrobium, Pseudomonas, and Hyphomicrobium in the RSBF, which have been affiliated with putative manganese-oxidizing bacteria (MnOB). The relative abundance of Pedomicrobium manganicum increased with operation time in both RSBF reactors. In addition, Nordella oligomobilis and Derxia gummosa were statistically correlated with Mn(II) oxidation. Species-species co-occurrence networks indicated that the microbiome of MnS-RSBF had more complex correlations than that of MagS-RSBF, implying that biofilter medium substantially shaped the microbial community in the RSBF. Hyphomicrobium and nitrite-oxidizing Nitrospira moscoviensis were positively correlated. The core microbiomes' composition of both RSBF reactors converged over operation time. A hybrid biofilter medium with magnetite sand and manganese sand may therefore be best in rapid sand filtration for Mn(II) oxidation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Compost-based biofiltration is a method widely used to mitigate ammonia emissions during composting. To improve the efficiency of a composting-biofiltration system, it is necessary to determine the most effective degree of composting at which to process the packing media used in the biofiltration system. In this study, materials pre-composted for 20 and 30 d (C20 and C30, respectively), and mature compost (CM) that had been treated for 60 d, were applied as biofilter media to remove ammonia from dairy manure composting exhaust gases. A comparison of the results revealed that the C30 biofilter not only completely removed ammonia, but also produced the least nitrogen loss (1.84%). The C20 biofilter exhibited an inferior performance, indicating that enough pre-composted time is necessary for material used as the packing media. Though the CM biofilter displayed good performance with regard to ammonia removal (97.8%), it had a high nitrogen loss (6.46%). A spearman rank correlation matrix revealed that the abundance of nitrogen cycle genes including amoA, nosZ, nirK, and nirS, had a strong correlation with the physicochemical properties such as nitrate content, carbon source, moisture content, and pH of the biofilter media. C30 provided advantageous conditions and contained a relatively high abundance of nitrifiers and the lowest abundance of denitrifiers. As a result, C30 rather than CM was a more appropriate biofilter media for ammonia removal. Moreover, the occurrence of biological nitrification during the dairy manure composting process indicates the effectiveness of a material for use as biofilter media., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Pilot testing of direct potable reuse (DPR) using multi-stage ozone and biological filtration as an alternative treatment train without reverse osmosis (RO) was investigated. This study examined four blending ratios of advanced treated reclaimed water from the F. Wayne Hill Water Resources Center (FWH WRC) in Gwinnett County, Georgia, combined with the existing drinking water treatment plant raw water supply, Lake Lanier, for potable water production. Baseline testing with 100 percent (%) Lake Lanier water was initially conducted; followed by testing blends of 15, 25, 50, and 100% reclaimed water from FWH WRC. Finished water quality from the DPR pilot was compared to drinking water standards, and emerging microbial and chemical contaminants were also evaluated. Results were benchmarked against a parallel indirect potable reuse (IPR) pilot receiving 100% of the raw water from Lake Lanier. Finished water quality from the DPR pilot at the 15% blend complied with the United States primary and secondary maximum contaminant levels (MCLs and SMCLs, respectively). However, exceedances of one or more MCLs or SMCLs were observed at higher blends. Importantly, reclaimed water from FWH WRC was of equal or better quality for all microbiological targets tested compared to Lake Lanier, indicating that a DPR scenario could lower acute risks from microbial pathogens compared to current practices. Finished water from the DPR pilot had no detections of microorganisms, even at the 100% FWH WRC effluent blend. Microbiological targets tested included heterotrophic plate counts, total and fecal coliforms, Escherichia coli, somatic and male-specific coliphage, Clostridium perfringens, Enterococci, Legionella, Cryptosporidium, and Giardia. There were water quality challenges, primarily associated with nitrate originating from incomplete denitrification and bromate formation from ozonation at the FWH WRC. These challenges highlight the importance of upstream process monitoring and control at the advanced wastewater treatment facility if DPR is considered. This research demonstrated that ozone with biological filtration could achieve potable water quality criteria, without the use of RO, in cases where nitrate is below the MCL of 10 mg nitrogen per liter and total dissolved solids are below the SMCL of 500 mg per liter., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Biofiltration has been observed to increase or decrease the concentrations of N-nitrosodimethylamine (NDMA) precursors in the effluents of full-scale drinking water facilities, but these changes have been inconsistent over time. Bench-scale tests comparing biofiltration columns side-by-side exposed to different conditions were employed to characterize factors associated with changes in NDMA precursor concentrations, as measured by application of chloramines under uniform formation conditions (UFC). Side-by-side comparisons of biofiltration media from different facilities fed with water from each of these facilities demonstrated that differences in source water quality were far more important than any original differences in the microbial communities on the biofiltration media for determining whether NDMA precursor concentrations increased, decreased or remained constant across biofilters. Additional tests involving spiking of specific constituents hypothesized to promote increases in NDMA precursor concentrations demonstrated that inorganic nitrogen species associated with nitrification, including ammonia, hydroxylamine and chloramines, and biotransformation of known precursors (i.e., municipal wastewater and the cationic polymer, polyDADMAC) to more potent forms were not important. Biotransformation of uncharacterized components of source waters determined whether NDMA precursor concentrations increased or decreased across biofilters. These uncharacterized source water component concentrations varied temporally and across locations. Where biotransformation of source water precursors increased NDMA precursor concentrations, ∼30-60% of the levels observed in column effluents fed with biofiltration influent water remained associated with the media and could be rinsed therefrom in either the dissolved or particulate form. Ozone pre-treatment significantly reduced NDMA precursor concentrations at one facility, suggesting that pre-oxidation could be an effective technique to mitigate the increase in NDMA precursor concentrations during biofiltration. Biofiltration decreased the concentrations of halogenated disinfection byproduct precursors., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

In this study, permeate quality, membrane performance, and microbial community in a gravity-driven microfiltration (GDM) reactor and a biofiltration (BF) + GDM reactor for seawater reverse osmosis (RO) desalination pretreatment were compared at both lab-scale and pilot-scale. The presence of BF column was more efficient in removing soluble organic substances by biosorption/biodegradation, leading to superior permeate quality from BF + GDM and subsequently lower RO fouling than GDM. Compared to the biofilm-saturated anthracite media, the granular activated carbon media in BF improved the assimilable organic substances removal in BF + GDM. Although less organic substances and microbial cells were accumulated on the membrane in BF + GDM, its permeate flux was 10-20% lower than GDM. Furthermore, BF lowered the amounts and diversity of prokaryotes (due to less organic substances) and eukaryotes (due to BF media rejection and lacking of prokaryotic preys) in the membrane biofilm of BF + GDM, but did not cause significant shifts of predominant species. Thus, the lower flux in BF + GDM was attributed to the limited predation and movement of eukaryotes in membrane biofilm, which may result in the formation of less porous and compact biofilm layer. The cost analysis indicated that BF + GDM-RO requires 5.2% less operating cost and 1.5% less water production cost than GDM-RO., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Water utilities must control microbial regrowth in the distribution system to protect public health. In this study, an adenosine triphosphate (ATP)-based biomass production potential test using indigenous bacterial communities were used to evaluate regrowth potential following ozonation with either biofiltration (BF) or sustained chlorination (SCl 2 ). Two full-scale water treatment plants with different upstream processes (i.e., WTP-BF: ozonation, coagulation/flocculation, biofiltration, UV irradiation, chlorination; and WTP-SCl 2 : ozonation, chlorination, coagulation/flocculation, filtration, chlorination) were compared. Characterization of indigenous bacteria using 16S rRNA gene sequencing, qPCR, and cellular ATP (cATP) showed microbial diversity changes across treatment, biomass sloughing from biofilters (effluent cATP = 30 ± 1 ng/L), and disinfection by chlorine (cATP < 1 ng/L). For both WTPs, 14-day cumulative biomass production (CBP t  =  ∑t=0tATP(t)×Δt) was highest for ozonated water samples (CBP 14  = 1.2 × 10 3 -3.0 × 10 3  d ngATP/L). CBP further increased with increasing ozone dose due to production of more biodegradable carbon. Growth promotion by carbon was confirmed from the consumption of ozonation byproducts (carboxylic acids, aldehydes) and the increase in CBP (9.5 × 10 2 -2.9 × 10 3  d ngATP/L) after addition of 50-300 μgC/L acetate. Ozone followed by sustained chlorination (WTP-SCl 2 ) effectively controlled biomass growth across the treatment process (CBP 14 <10 d ngATP/L). In contrast, ozone followed by biofiltration (WTP-BF) reduced regrowth potential by 30% (biofilter influent CBP 14  = 1.3 × 10 3  d ngATP/L; biofilter effluent CBP 14  = 9.3 × 10 2  d ngATP/L). After adding chlorine to the biofilter effluent, CBP 14 was reduced to <10 d ngATP/L. Lastly, online ATP measurements confirmed the discrete measurements and improved identification of the cATP peak and growth phases of indigenous bacteria., (Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

The biological degradation of many trace organic compounds has been reported to be strongly redox dependent. The traditional characterization of redox conditions using the succession of inorganic electron acceptors such as dissolved oxygen and nitrate falls short in accurately describing the critical transition state between oxic and suboxic conditions. Novel monitoring strategies using intrinsic redox tracers might be suitable to close that gap. This study investigated the potential use of the successive biological transformation of the iodinated contrast medium iopromide as an intrinsic tracer of prevailing redox conditions in biofiltration systems. Iopromide degradation in biofiltration systems was monitored by quantifying twelve known biological transformation products formed under oxic conditions. A novel dimensionless parameter (T IOP ) was introduced as a measure for the successive transformation of iopromide. A strong correlation between the consumption of dissolved oxygen and iopromide transformation emphasized the importance of general microbial activity on iopromide degradation. However, results disproved a direct correlation between oxic (>1 mg/L O 2 ) and suboxic (<1 mg/L O 2 ) conditions and the degree of iopromide transformation. Results indicated that besides redox conditions also the availability of biodegradable organic substrate affects the degree of iopromide transformation. Similar behavior was found for the compounds gabapentin and benzotriazole, while the oxic degradation of metoprolol remained stable under varying substrate conditions., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The combination of biological growth and particle loading can adversely affect hydraulic performance in drinking water biofilters. In this study, upstream oxidant addition was used to distribute biologically-derived filter clogging in granular activated carbon (GAC) biofilters. Oxidant penetration was assessed during pilot-scale operation and backwashing of dual media (GAC/sand) and multimedia (GAC/anthracite/sand) biofilters. Influent chlorine (HOCl), monochloramine (NH 2 Cl), and hydrogen peroxide (H 2 O 2 ) residuals were optimized to react with the GAC surface in the upper portion of the filter media bed (depth < 0.5 m) to attenuate biomass development. As the oxidant residual was quenched by surface-mediated reaction with the filter media, biomass growth was promoted deeper in the filter bed (depth > 0.5 m). The oxidant-induced effects on biomass and hydraulic performance were monitored through measurements of adenosine triphosphate (ATP) and head loss accumulation at different media depths. Addition of oxidants (e.g., 0.6 mg Cl 2 /L HOCl) could decrease terminal head loss by 20% in dual media filters and 40% in multimedia filters. These hydraulic benefits were achieved without significantly affecting removal of assimilable organic carbon (AOC), total organic carbon (TOC), turbidity, and particle counts. Oxidant type, residual concentration, media type, media age, and media depth influenced the passage of oxidant residuals and distribution of filter biomass. When oxidants were added during backwashing, oxidant residual was quenched through the bed depth from a combination of reactions with GAC media and biofilm degradation. This attenuation of residual oxidant may prevent the oxidant residual from penetrating the entire bed depth, potentially compromising backwashing objectives., (Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The treatment efficiency of a vegetative filter strip and a biofiltration swale treating heavily loaded road runoff are evaluated. Concentrations measured in water drained from the two systems are compared to those in untreated road runoff collected from a reference catchment for a wide range of contaminants including organic carbon, nutrients (N and P), trace metals, and organic micropollutants (polycyclic aromatic hydrocarbons (PAH), total petroleum hydrocarbons (TPH), alkylphenols, bisphenol-A, phthalates), in both total and dissolved phases. Predominantly particulate pollutants, including Pb, Zn and PAH, were very efficiently removed (around 90%) for most events. However, poor particulate removal was observed during a winter period. Relatively few pollutants were significantly removed in the dissolved phase and observed concentration reductions tended to be lower than those of suspended solids and associated pollutants; as such, lower removals were observed for total concentrations of moderately particulate micropollutants, including bisphenol-A, alkylphenols and phthalates. In addition, some pollutants appear to be emitted from various biofilter components (filter media, drainage and lining materials), as low or negative concentration removals were observed during the first months of operation of the biofiltration swale., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

The performance of biofiltration to mitigate CH 4 emissions from cow barns was investigated in the laboratory using two flow-through columns constructed with an acclimatized packed bed media composed of inexpensive materials and readily available in an agricultural context. The biofilters were fed with artificial exhaust gas at a constant rate of 0.036 m 3  h -1 and low inlet CH 4 concentration (0.22 g m -3  = 300 ppm). The empty-bed residence time (EBRT) was equal to 0.21 h. Additionally, in order to simulate temperature changes under natural conditions and determine the influence of such cycles on CH 4 removal efficiency, the upper part of the biofilters were submitted to temperature oscillations over time. The maximum oxidation rate (1.68 μg CH4 g dw -1 h -1 ) was obtained with the commercial compost mixed with straw. Accordingly, it was considered as packing bed media for the biofilters. The CH 4 removal efficiency was affected by the temperature prevailing within the biofilters, by the way in which the cooling-warming cycles were applied and by the acclimatization process. The shorter the cooling-warming cycles, the more oxidation rates varied. With longer cycles, CH 4 removal rates stabilized and CH 4 removal efficiencies attained nearly 100% in both biofilters, and remained at this level for more than 100 days, irrespective of the temperature at the top of the biofilter, which was - at times - adverse for microbiological activity. The first order rate constant for CH 4 . If such rate could be transposed to real field conditions in Canada, home to nearly 945,000 dairy cows, biofiltration may be applied to efficiently abate between 2 × 10 -1 . If such rate could be transposed to real field conditions in Canada, home to nearly 945,000 dairy cows, biofiltration may be applied to efficiently abate between 2 × 10 6 and 3 × 10 6  t yr -1 of CO 2 equivalent (depending on how estimates are performed) from bovine enteric fermentation alone., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

The use of gadolinium-based contrasting agents (GBCA) is increasing because of the intensive usage of these agents in magnetic resonance imaging (MRI). Waste-water treatment does not reduce anthropogenic Gd-concentration significantly. Anomalous Gd-concentration in surface waters have been reported worldwide. However, removal of GBCA-s by aquatic macrophytes has still hardly been investigated. Four aquatic plant species (Lemna gibba, Ceratophyllum demersum, Elodea nuttallii, E. canadensis) were investigated as potential biological filters for removal of commonly used but structurally different GBCA-s (Omniscan, Dotarem) from water. These plant species are known to accumulate heavy metals and are used for removing pollutants in constructed wetlands. The Gd uptake and release of the plants was examined under laboratory conditions. Concentration-dependent infiltration of Gd into the body of the macrophytes was measured, however significant bioaccumulation was not observed. The tissue concentration of Gd reached its maximum value between day one and four in L. gibba and C. demersum, respectively, and its volume was significantly higher in C. demersum than in L. gibba. In C. demersum, the open-chain ligand Omniscan causes two-times higher tissue Gd concentration than the macrocyclic ligand Dotarem. Gadolinium was released from Gd-treated duckweeds into the water as they were grown further in Gd-free nutrient solution. Tissue Gd concentration dropped by 50% in duckweed treated by Omniscan and by Dotarem within 1.9 and 2.9 days respectively. None of the macrophytes had a significant impact on the Gd concentration of water in low and medium concentration levels (1-256 μg L -1 ). Biofiltration of GBCA-s by common macrophytes could not be detected in our experiments. Therefore it seems that in constructed wetlands, aquatic plants are not able to reduce the concentration of GBCA-s in the water. Furthermore there is a low risk that these plants cause the accumulation of anthropogenic Gd in the food chain., (Copyright © 2017. Published by Elsevier Ltd.)

Microbial community structure in the ozone-biofiltration systems of two drinking water and two wastewater treatment facilities was characterized using 16S rRNA gene sequencing. Collectively, these datasets enabled comparisons by facility, water type (drinking water, wastewater), pre-oxidation (ozonation, chlorination), media type (anthracite, activated carbon), media depth, and backwash dynamics. Proteobacteria was the most abundant phylum in drinking water filters, whereas Bacteroidetes, Chloroflexi, Firmicutes, and Planctomycetes were differentially abundant in wastewater filters. A positive correlation was observed between media depth and relative abundance of Cyanobacteria in drinking water filters, but there was only a slight increase in one alpha diversity metric with depth in the wastewater filters. Media type had a significant effect on beta but not alpha diversity in drinking water and wastewater filters. Pre-ozonation caused a significant decrease in alpha diversity in the wastewater filters, but the effect on beta diversity was not statistically significant. An evaluation of backwash dynamics resulted in two notable observations: (1) endosymbionts such as Neochlamydia and Legionella increased in relative abundance following backwashing and (2) nitrogen-fixing Bradyrhizobium dominated the microbial community in wastewater filters operated with infrequent backwashing. Bradyrhizobium is known to generate extracellular polymeric substances (EPS), which may adversely impact biofilter performance and effluent water quality. These findings have important implications for public health and the operation and resiliency of biofiltration systems., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Landfill leachates are known for their high and complex composition of organic, inorganic and microbial pollutants. As a result, it is quite challenging to treat these effluents by using only one treatment process. A combining approach is generally required to treat efficiently these wastewaters and comply with the discharge standards. In this present study, electrocoagulation (EC) and biofiltration (BF) processes were sequentially used to treat landfill leachate. EC process has been able to remove 37 ± 2% of the initial total COD. A fractionation of organic compounds showed that EC was particularly efficient to remove insoluble COD and humic acids. In addition, other pollutants such as turbidity, true color, Zn and phosphorus were significantly reduced by EC with 82 ± 2.7%, 60 ± 13%, 95 ± 2.6% and 82 ± 5.5% of removal respectively. The subsequent treatment by BF process led to completely removal of ammonia pollution (>99% of NH 4 removal) and a partial removal of dissolved organic compounds (42 ± 7% of COD removal). The hybrid process EC/BF could form the basis of a process capable of removing organic and inorganic pollutants from many refractory wastewaters (mature landfill leachates, industrial and municipal wastewaters)., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

In this study, a fixed-bed biofiltration system (biofilter) that utilized indigenous microorganisms was developed for the reclamation of oil sands process-affected water (OSPW). With the assistance of quantitative polymerase chain reaction (qPCR) and confocal laser scanning microscopy (CLSM), indigenous microorganisms from OSPW were able to attach to the surface of sand media and form biofilms. The number of total bacteria on the biofilter media reached a steady state (10 9 /g) after 23 days of operation. Ultra Performance Liquid Chromatography/High Resolution Mass Spectrometry (UPLC/HRMS) analysis showed that 21.8% of the classical naphthenic acids (NAs) removal was achieved through the circulation of raw OSPW on the biofilter for 8 times (equivalent to a hydraulic retention time of 16 h). When ozonation with utilized ozone dose of 30 mg/L was applied as pretreatment, the classical NAs in the ozonated OSPW were removed by 89.3% with an accelerated biodegradation rate of 0.5 mg/L/h. Compared with other biofilm reactors such as moving bed biofilm reactor (MBBR), ozonation pretreatment could benefit the biodegradation of NAs in the biofilter more (classical NA removal: 89.3% vs. 34.4%), especially for those with high carbon number and cyclicity. The combined ozonation-biofiltration process could remove 92.7% of classical NAs from raw OSPW in 16 h. Although both ozonation and biofiltration alone did not show degradation of oxidized NAs from raw OSPW, the combined process led to a 52.9% and 42.6% removal for O 3 -NAs and O 4 -NAs, respectively, which were the dominant oxidized NA species in OSPW. Metagenomic sequencing analysis showed that Rhodococcus was the dominant bacterial genus on the sand media, which may play a crucial role during the NA biodegradation. With the advantage of high NA removal efficiency, the combined ozonation-biofiltration process is a promising approach for NA degradation and shows high potential to be scaled up for in-situ OSPW treatment., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Biodegradable organic matter (BOM), found in all surface waters, is a challenge for drinking water utilities because it can lead to distribution system bio-regrowth, react to form disinfection by-products, or be a specific compound of concern. A critical review of BOM (occurrence and oxidant effects) and rapid-rate biofiltration performance (preozonation, backwashing with an oxidant, empty bed contact time (EBCT) and temperature) was carried out. An extensive literature data analysis (n = 100) found total organic carbon (TOC) in nonozonated water is comprised of 20% (median) biodegradable organic carbon (BDOC) and 3% (median) assimilable organic carbon (AOC). For ozonated waters (n = 103), these values increased to 30% (median) BDOC and 9% (median) AOC. For all operation conditions (n = 117), biofilters (12 min average EBCT) removed 12% (median) of the influent TOC with higher removals for ozonated waters, 15% (median), compared to nonozonated waters, 10% (median). As temperature increased from ≤10 °C to ≥20 °C, TOC removal increased from 10% to 17% (median). This review demonstrates biofiltration can be an efficient treatment technology to remove a portion of the BOM from the filter influent and should be optimized to achieve maximum removal., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Media type is a critical design consideration when implementing biofiltration for drinking water treatment. Granular activated carbon (GAC) has been shown to provide superior performance when compared to a wide range of media types, largely due to its higher surface area. Engineered ceramic media is an attractive alternative to GAC as it has a similar surface area but at a lower cost. This pilot-scale biofiltration study compared the performance of GAC, anthracite and two different effective sizes of ceramic (CER) media (1.0 mm and 1.2 mm), in terms of dissolved organic carbon (DOC), head loss, turbidity, and disinfection by-product formation potential (DBPFP). Biological acclimation was monitored using adenosine tri-phosphate (ATP) measurements; biomass was further examined using laccase and esterase enzyme activity assays. When compared to other media types examined, biological GAC had higher (p > 0.05) removals of DOC (9.8 ± 3.8%), trihalomethane formation potential (THMFP, 26.3 ± 10.2%), and haloacetic acid formation potential (HAAFP, 27.2 ± 14.0%). CER media required 6-7 months to biologically acclimate, while filters containing GAC and anthracite were biologically active (>100 ng of ATP/g media) following 30-45 days of operation. Once acclimated, ATP values of 243 and 208 ng/g attained for CER 1.0 and 1.2, respectively, were statistically comparable to GAC (244 ng/g) and higher than anthracite (110 ng/g), however this did not translate into greater organics removal. Esterase and laccase enzyme kinetics were highest for GAC, while CER was shown to have greater biodegradation potential than anthracite. The four media types attained similar turbidity reduction (p > 0.05), however ceramic media filters were observed to have run times which were 1.5-2.3 times longer when compared to anthracite, which could represent potential cost savings in terms of energy for pumping and backwash requirements. Overall, ceramic media was shown to be a potential alternative to anthracite when considering biofiltration, especially during cold water conditions (T < 10 °C)., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Microorganisms inhabiting filtration media of a drinking water treatment plant can be beneficial, because they metabolize biodegradable organic matter from source waters and those formed during disinfection processes, leading to the production of biologically stable drinking water. However, which microbial consortia colonize filters and what metabolic capacity they possess remain to be investigated. To gain insights into these issues, we performed metagenome sequencing and analysis of microbial communities in three different filters of a full-scale drinking water treatment plant (DWTP). Filter communities were sampled from a rapid sand filter (RSF), granular activated carbon filter (GAC), and slow sand filter (SSF), and from the Schmutzdecke (SCM, a biologically active scum layer accumulated on top of SSF), respectively. Analysis of community phylogenetic structure revealed that the filter bacterial communities significantly differed from those in the source water and final effluent communities, respectively. Network analysis identified a filter-specific colonization pattern of bacterial groups. Bradyrhizobiaceae were abundant in GAC, whereas Nitrospira were enriched in the sand-associated filters (RSF, SCM, and SSF). The GAC community was enriched with functions associated with aromatics degradation, many of which were encoded by Rhizobiales (∼30% of the total GAC community). Predicting minimum generation time (MGT) of prokaryotic communities suggested that the GAC community potentially select fast-growers (<15 h of MGT) among the four filter communities, consistent with the highest dissolved organic matter removal rate by GAC. Our findings provide new insights into the community phylogenetic structure, colonization pattern, and metabolic capacity that potentially contributes to organic matter removal achieved in the biofiltration stages of the full-scale DWTP., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Recent studies revealed the benefits of oligotrophic and oxic conditions for the biological removal of many trace organic chemicals (TOrCs) during soil-aquifer treatment. These findings indicate an unused tuning potential that might also be applicable in engineered biofiltration systems with drastically reduced hydraulic retention times for an enhanced mitigation of TOrCs during wastewater treatment. This study introduces the novel approach of sequential biofiltration (SBF) for the advanced treatment of secondary effluent using two granular media filters operated in series with an intermediate aeration step aiming for oxic and oligotrophic conditions in the second filter stage. Results from the experiments conducted at pilot-scale confirm a reduced substrate availability and predominantly oxic conditions in the second filter stage of the SBF setup. An increased removal of several TOrCs was observed in an SBF system as compared to a conventional single-stage biofiltration unit operated at the same overall empty bed contact time (EBCT). Short-term tests with varying EBCTs in the first filter stage revealed a high degree of system robustness of TOrC mitigation when confronted with sudden hydraulic load variations. Higher removal of several TOrCs at increased EBCTs in the second filter stage indicates that EBCT might play a crucial role for the degradation of certain compounds., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

While disinfection provides hygienically safe drinking water, the disinfectants react with inorganic or organic precursors, leading to the formation of harmful disinfection byproducts (DBPs). Biological filtration is a process in which an otherwise conventional granular filter is designed to remove not only fine particulates but also dissolved organic matters (e.g., DBP precursors) through microbially mediated degradation. Recently, applications of biofiltration in drinking water treatment have increased significantly. This review summarizes the effectiveness of biofiltration in removing DBPs and their precursors and identifies potential factors in biofilters that may control the removal or contribute to formation of DBP and their precursors during drinking water treatment. Biofiltration can remove a fraction of the precursors of halogenated DBPs (trihalomethanes, haloacetic acids, haloketones, haloaldehydes, haloacetonitriles, haloacetamides, and halonitromethanes), while also demonstrating capability in removing bromate and halogenated DBPs, except for trihalomethanes. However, the effectiveness of biofiltration mediated removal of nitrosamine and its precursors appears to be variable. An increase in nitrosamine precursors after biofiltration was ascribed to the biomass sloughing off from media or direct nitrosamine formation in the biofilter under certain denitrifying conditions. Operating parameters, such as pre-ozonation, media type, empty bed contact time, backwashing, temperature, and nutrient addition may be optimized to control the regulated DBPs in the biofilter effluent while minimizing the formation of unregulated emerging DBPs. While summarizing the state of knowledge of biofiltration mediated control of DBPs, this review also identifies several knowledge gaps to highlight future research topics of interest., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Four dual media filters (DMFs) were operated in a biofiltration mode with different engineered environments (DMF I and II: coagulation with/without acidification and DMF III and IV: without/with chlorination). Designed biofilm enrichment reactors (BERs) containing the removable reverse osmosis (RO) coupons, were connected at the end of the DMFs in parallel to analyze the biofilm on the RO membrane by DMF effluents. Filtration performances were evaluated in terms of dissolved organic carbon (DOC) and assimilable organic carbon (AOC). Organic foulants on the RO membrane were also quantified and fractionized. The bacterial community structures in liquid (seawater and effluent) and biofilm (DMF and RO) samples were analyzed using 454-pyrosequencing. The DMF IV fed with the chlorinated seawater demonstrated the highest reductions of DOC including LMW-N as well as AOC among the other DMFs. The DMF IV was also effective in reducing organic foulants on the RO membrane surface. The bacterial community structure was grouped according to the sample phase (i.e., liquid and biofilm samples), sampling location (i.e., DMF and RO samples), and chlorination (chlorinated and non-chlorinated samples). In particular, the biofilm community in the DMF IV differed from the other DMF treatments, suggesting that chlorination exerted as stronger selective pressure than pH adjustment or coagulation on the biofilm community. In the DMF IV, several chemoorganotrophic chlorine-resistant biofilm-forming bacteria such as Hyphomonas, Erythrobacter, and Sphingomonas were predominant, and they may enhance organic carbon degradation efficiency. Diverse halophilic or halotolerant organic degraders were also found in other DMFs (i.e., DMF I, II, and III). Various kinds of dominant biofilm-forming bacteria were also investigated in RO membrane samples; the results provided possible candidates that cause biofouling when DMF process is applied as the pretreatment option for the RO process., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

The removal of precursors of 36 disinfection byproducts (DBPs) in effluents from flocculation/sedimentation process was evaluated across a pilot-scale two-stage biofiltration process, i.e., a sand/anthracite (SA) biofilter (empty bed contact time (EBCT) of 7.5 min) coupled with a biologically-active granular activated carbon (GAC) contactor (EBCT of 15 min). The biofiltration process exhibited a good capacity for removal of the total DBP formation potential (DBPFP) (by 25.90 ± 2.63%), and GAC contactors contributed most to the DBPFP removal (accounting for 60.63 ± 16.64% of the total removal). The removal percentage of DBPFPs of different structure types was in the following order: halonitroalkanes (58.50%) > haloaldehydes (33.62%) > haloacetic acids (HAAs, 28.13%) > haloalkanes (20.46%) > haloketones (13.46%) > nitrosamines (10.23%) > halonitriles (-8.82%) > haloalkenes (-9.84%). The precursors of bromo-DBPs (containing only bromine atoms) and maximal halogenated DBPs (containing 3 & 4 halo atoms) were removed largely compared to other DBPs. Among the total DBPFP, trihalomethanes (THMs), HAAs, and chloral hydrate were the dominant DBPs, and they accounted for >92% of the total targeted DBPs by weight. Pearson correlation analysis (CA) and principal components analysis (PCA) indicated a significant association among these dominant DBPs. Canonical correspondence analysis (CCA) revealed specific ultraviolet absorbance (SUVA 254 ) could serve as a good surrogate parameter for DBPFP. Pre-chlorination upstream of the biofilters may not greatly impact the overall removal of DBPFP by SA/GAC biofiltration. In addition, results showed that SA/GAC biofiltration was a useful procedure to remove the inorganic DBP chlorite., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Global water resources contain a variety of micropollutants (MPs), including pharmaceuticals, personal care products, and pesticides. This study investigated the removal of MPs during drinking water production by means of biofiltration. The objective of this work was to investigate the influence of hydrodynamics on biofilm growth and development in a biofiltration process and the consequent effect on MP biotransformation rates. We operated three groups of biofiltration columns continuously for 381 days under three distinct hydrodynamic regimes (superficial velocity: 10, 20, 40 cm h -1 ) and fed them a mixture of 29 micropollutants at low concentrations. Total protein concentrations were used as a surrogate measurement for attached biomass and periodic tracer experiments were conducted to estimate dispersivity and assess changes in the depth of the biological zone in each biofilter. These data revealed significant differences in biofilm assembly among the biofilters; higher superficial velocities led to less concentrated surface biomass but a deeper biological zone and more total biomass. Eleven of the 29 MPs were biotransformed and nine of those could be evaluated to estimate biotransformation rates. The second-order rate constants for all nine MPs were not significantly different among the hydrodynamic regimes. However, a depth-based analysis of biotransformation rates revealed significantly greater second-order rate constants for 5 of the MPs at increasing biofilter depths, suggesting that sparse microbial communities found in deeper and more oligotrophic biofilters had a greater activity for the biotransformation of these MPs. The identification of several transformation products at similar relative distributions suggests that the greater activity was not the result of changing metabolic processes under more oligotrophic conditions. These results improve our fundamental understanding of biofilm assembly and functioning in biofiltration processes., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Water treatment combining advanced oxidative processes with subsequent exposure to biological activated carbon (BAC) holds promise for the attenuation of recalcitrant pollutants. Here we contrast oxidation and subsequent biofiltration of treated wastewater effluent employing either ozone or UV/H 2 O 2 followed by BAC during pilot-scale implementation. Both treatment trains largely met target water quality goals by facilitating the removal of a suite of trace organics and bulk water parameters. N-nitrosodimethylamine (NDMA) formation was observed in ozone fed BAC columns during biofiltration and to a lesser extent in UV/H 2 O 2 fed columns and was most pronounced at 20 min of empty bed contact time (EBCT) when compared to shorter EBCTs evaluated. While microbial populations were highly similar in the upper reaches, deeper samples revealed a divergence within and between BAC filtration systems where EBCT was identified to be a significant environmental predictor for shifts in microbial populations. The abundance of Nitrospira in the top samples of both columns provides an explanation for the oxidation of nitrite and corresponding increases in nitrate concentrations during BAC transit and support interplay between nitrogen cycling with nitrosamine formation. The results of this study demonstrate that pretreatments using ozone versus UV/H 2 O 2 impart modest differences to the overall BAC microbial population structural and functional attributes, and further highlight the need to evaluate NDMA formation prior to full-scale implementation of BAC in potable reuse applications., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Greywater is being increasingly used as an alternative water source to reduce potable water demand and to alleviate pressure on sewerage systems. This paper presents the development of a low energy and low maintenance greywater treatment technology: a living wall system, employing ornamental plants (including vines) grown in a sand filter on a side of a building to treat shower, bath, and washing basin wastewaters. The system can, at the same time, provide critical amenity and micro-climate benefits to our cities. A large scale column study was conducted in Melbourne, Australia, to investigate the following design and operational factors of the proposed system: plant species, saturated zone design, rest period, hydraulic loading rate and pollutant inflow concentration. The results indicate that the use of ornamental species (e.g. Canna lilies, Lonicera japonica, ornamental grape vine) can contribute to pollutant removal. Vegetation selection was found to be particularly important for nutrient removal. While a wider range of tested plant species was effective for nitrogen removal (>80%), phosphorus removal was more variable (-13% to 99%) over the study period, with only a few tested plants being effective - Carex appressa and Canna lilies were the best performers. It was also found that phosphorus removal can be compromised over the longer term as a result of leaching. Excellent suspended solids and organics removal efficiencies can be generally achieved in these systems (>80% for TSS and >90% for BOD) with plants having a relatively small impact. Columns had an acceptable infiltration capacity after one year of operation. When planted with effective species (e.g. Carex appressa and Canna lilies), it is expected that performance will not be significantly affected by longer rest periods and higher pollutant concentrations in the early years of system operation. The results of this study, thus, demonstrate that innovative and aesthetically pleasing living walls can be designed for treatment of greywater at the household scale., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Ozonation is known to generate biodegradable organic matter, which is typically reduced by biological filtration to avoid bacterial regrowth in distribution systems. Post-chlorination generates halogenated disinfection byproducts (DBPs) but little is known about the biodegradability of their precursors. This study determined the effect of ozonation and biofiltration conditions, specifically ozone exposure and empty bed contact time (EBCT), on the control of DBP formation potentials in drinking water. Ozone exposure was varied through addition of H 2 O 2 during ozonation at 1 mgO 3 /mgDOC followed by biological filtration using either activated carbon (BAC) or anthracite. Ozonation led to a 10% decrease in dissolved organic carbon (DOC), without further improvement from H 2 O 2 addition. Raising H 2 O 2 concentrations from 0 to 2 mmol/mmolO 3 resulted in increased DBP formation potentials during post-chlorination of the ozonated water (target Cl 2 residual after 24 h = 1-2 mg/L) as follows: 4 trihalomethanes (THM4, 37%), 8 haloacetic acids (HAA8, 44%), chloral hydrate (CH, 107%), 2 haloketones (HK2, 97%), 4 haloacetonitriles (HAN4, 33%), trichloroacetamide (TCAM, 43%), and adsorbable organic halogen (AOX, 27%), but a decrease in the concentrations of 2 trihalonitromethanes (THNM2, 43%). Coupling ozonation with biofiltration prior to chlorination effectively lowered the formation potentials of all DBPs including CH, HK2, and THNM2, all of which increased after ozonation. The dynamics of DBP formation potentials during BAC filtration at different EBCTs followed first-order reaction kinetics. Minimum steady-state concentrations were attained at an EBCT of about 10-20 min, depending on the DBP species. The rate of reduction in DBP formation potentials varied among individual species before reaching their minimum concentrations. CH, HK2, and THNM2 had the highest rate constants of between 0.5 and 0.6 min -1 followed by HAN4 (0.4 min -1 ), THM4 (0.3 min -1 ), HAA8 (0.2 min -1 ), and AOX (0.1 min -1 ). At an EBCT of 15 min, the reduction in formation potential for most DBPs was less than 50% but was higher than 70% for CH, HK2, and THNM2. The formation of bromine-containing DBPs increased with increasing EBCT, most likely due to an increase in Br - /DOC ratio. Overall, this study demonstrated that the combination of ozonation and biofiltration is an effective approach to mitigate DBP formation during drinking water treatment., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Potable water treatment in small communities is challenging due to a complexity of factors starting with generally poor raw water sources, a smaller tax and consumption base that limit capital and operating funds, and culminating in what is typically a less sophisticated and robust water treatment plant for production and delivery of safe, high quality potable water. The design and optimization of modular ozone-assisted biological filtration systems can address some of these challenges. In surface water treatment, the removal of organic matter (e.g., dissolved organic carbon - DOC), inorganic nutrients and other exposure-related contaminants (e.g., turbidity and dissolved solids) from the raw water source is essential. Thus, a combination of chemical and biological oxidation processes can produce an effective and efficient water treatment plant design that is also affordable and robust. To that end, the ozone-assisted biological filtration water treatment plants in two communities were evaluated to determine the efficacy of oxidation and contaminant removal processes. The results of testing for in-field system performance indicate that plant performance is particularly negatively impacted by high alkalinity, high organics loading, and turbidity. Both bicarbonate and carbonate alkalinity were observed to impede ozone contact and interaction with DOC, resulting in lower than anticipated DOC oxidation efficiency and bioavailability. The ozone dosage at both water treatment plants must be calculated on a more routine basis to better reflect both the raw water DOC concentration and presence of alkalinities to ensure maximized organics oxidation and minimization of trihalomethanes production., (Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.)

To better understand biofiltration, concentration profiles of various natural organic matter (NOM) components throughout a pilot-scale drinking water biofilter were investigated using liquid chromatography - organic carbon detection (LC-OCD) and fluorescence excitation and emission matrices (FEEM). Over a 2 month period, water samples were collected from six ports at different biofilter media depths. Results showed substantial removal of biopolymers (i.e. high molecular weight (MW) NOM components as characterized by LC-OCD) and FEEM protein-like materials, but low removal of humic substances, building blocks and low MW neutrals and low MW acids. For the first time, relative biodegradability of different NOM components characterized by LC-OCD and FEEM approaches were investigated across the entire MW range and for different fluorophore compositions, in addition to establishing the biodegradation kinetics. The removal kinetics for FEEM protein-like materials were different than for the LC-OCD-based biopolymers, illustrating the complementary nature of the LC-OCD and FEEM approaches. LC-OCD biopolymers (both organic carbon and organic nitrogen) and FEEM protein-like materials were shown to follow either first or second order biodegradation kinetics. Due to the low percent removal and small number of data points, the performance of three kinetic models was not distinguishable for humic substances. Pre-filtration of samples for FEEM analyses affected the removal behaviours and/or kinetics especially of protein-like materials which was attributed to the removal of the colloidal/particulate materials., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

A full-scale nitrifying biofilter was continuously monitored during two measurement periods (September 2014; February 2015) during which both gaseous and liquid N2O fluxes were monitored on-line. The results showed diurnal and seasonal variations of N2O emissions. A statistical model was run to determine the main operational parameters governing N2O emissions. Modification of the distribution between the gas phase and the liquid phase was observed related to the effects of temperature and aeration flow on the volumetric mass transfer coefficient (kLa). With similar nitrification performance values, the N2O emission factor was twice as high during the winter campaign. The increase in N2O emissions in winter was correlated to higher effluent nitrite concentrations and suspected increased biofilm thickness., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

In surface water treatment, ultrafiltration (UF) membranes are widely used because of their ability to supply safe drinking water. Although UF membranes produce high-quality water, their efficiency is limited by fouling. Improving UF filtrate productivity is economically desirable and has been attempted by incorporating sustainable biofiltration processes as pretreatment to UF with varying success. The availability of models that can be applied to describe the effectiveness of biofiltration on membrane mass transfer are lacking. In this work, UF water productivity was empirically modeled as a function of biofilter feed water quality using either a quadratic or Gaussian relationship. UF membrane mass transfer variability was found to be governed by the dimensionless mass ratio between the alkalinity (ALK) and dissolved organic carbon (DOC). UF membrane productivity was optimized when the biofilter feed water ALK to DOC ratio fell between 10 and 14., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

Biofiltration systems are used in urban areas to reduce the concentration and load of nutrient pollutants and heavy metals entering waterways through stormwater runoff. Biofilters can, however be exposed to salt water, through intrusion of seawater in coastal areas which could decrease their ability to intercept and retain pollutants. We measured the effect of adding saline stormwater on pollutant removal by six monocotyledonous species with different levels of salt-tolerance. Carex appressa, Carex bichenoviana, Ficinia nodosa, Gahnia filum, Juncus kraussii and Juncus usitatus were exposed to six concentrations of saline stormwater, equivalent to electrical conductivity readings of: 0.09, 2.3, 5.5, 10.4, 20.0 and 37.6 mS cm(-1). Salt-sensitive species: C. appressa, C. bichenoviana and J. usitatus did not survive ≥10.4 mS cm(-1), removing their ability to take up nitrogen (N). Salt-tolerant species, such as F. nodosa and J. kraussii, maintained N-removal even at the highest salt concentration. However, their levels of water stress and stomatal conductance suggest that N-removal would not be sustained at concentrations ≥10.4 mS cm(-1). Increasing salt concentration indirectly increased phosphorus (P) removal, by converting dissolved forms of P to particulate forms which were retained by filter media. Salt concentrations ≥10 mS cm(-1) also reduced removal efficiency of zinc, manganese and cadmium, but increased removal of iron and lead, regardless of plant species. Our results suggest that biofiltration systems exposed to saline stormwater ≤10 mS cm(-1) can only maintain N-removal when planted with salt-tolerant species, while P removal and immobilisation of heavy metals is less affected by species selection., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

A tertiary treatment step is required in current wastewater treatment plants to remove trace pollutants and thus to prevent their extensive occurrence in the aquatic environment. In this study, natural MnOx ore and natural zeolite were separately used to pack two lab-scale aerated biofilters, which were operated in approximately 1.5 years for the removal of frequently occurring pharmaceuticals, including carbamazepine (CBZ), diclofenac (DFC), and sulfamethoxazole (SMX), out of synthetic and real secondary effluents. Mn(2+) was added in the feeds to promote the growth of iron/manganese oxidizing bacteria which were recently found to be capable of degrading recalcitrant pollutants. An effective removal (80-90%) of DFC and SMX was observed in both biofilters after adaptation while a significant removal of CBZ was not found. Both biofilters also achieved an effective removal of spiked Mn(2+), but a limited removal of carbon and nitrogen contents. Additionally, MnOx biofilter removed 50% of UV254 from real secondary effluent, indicating a high potential on the removal of aromatic compounds., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

The objective of this study was to determine the extent to which a suite of organic micropollutants (MPs) can be removed by biological filtration and the role of bioavailability and ammonia oxidizing microorganisms (AOMs) in the biodegradation process. During approximately one year, laboratory-scale columns with 8 min empty bed contact time (EBCT) and packed with anthracite as filter media were used for treating a tertiary effluent spiked with a broad range of MPs at a target concentration of 2 μg L(-1). In parallel columns, aerobic biomass growth was inhibited by using either the biocide sodium azide (500 mg L(-1) NaN3) or allylthiourea (5 mg L(-1) ATU), specifically inhibiting nitrifying bacteria. Once the biomass had colonized the media, around 15% of the dissolved organic carbon (DOC) contained in the untreated tertiary effluent was removed by non-inhibited columns. The removal of several MPs increased over time indicating the relevance of biological activity for the removal of MPs, while the negative control, the NaN3 inhibited column, showed no significant removal. Out of 33 MPs, 19 were recalcitrant (<25%) to biodegradation under aerobic conditions with the others exhibiting a diverse range of removal efficiency up to 95%. Through inhibition by ATU it was shown that nitrifying bacteria were clearly having a role in the degradation of several MPs, whereas the removal of other MPs was not affected by the presence of the nitrification inhibitor. A relationship between the qualitative assessment of sorption of MPs on granular activated carbon (GAC) and their removal efficiency by biodegradation on anthracite was observed. This result suggested that the affinity of the MPs for GAC media could be a useful indicator of the bioavailability of compounds during biofiltration on anthracite., (Copyright © 2014 Elsevier Ltd. All rights reserved.)