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We determined a trace amount of 14 phenols derived from pesticides by high performance liquid chromatography with an electrochemical detector. Phenols from aqueous and solid samples were enriched through exhaustive steam distillation and XAD-4 resin adsorption. Quantitative determinations were done by reverse-phase high performance liquid chromatography without derivatization. The minimum detectable quantity: phenol, 0.1ng; 2, 4-dichlorophenol, 0.4ng; 2, 4, 6-trichlorophenol, 0.4ng; 2-nitrophenol, 0.4ng. The recovery of phenols from egg plant samples fortified with each of 4-chlorophenol, 4-chloro-2-methylphenol and 2, 4-dichlorophenol at 1ppm ranged from 78 to 102%, while that of 4-nitro-phenol was poor. The recovery of 3-methyl-4-nitrophenol was not determined because other materials interfered.

We have developed a method to determine anilines derived from pesticides by high performance liquid chromatography with an electrochemical detector. Pesticides were hydrolyzed with HCl or KOH to the corresponding anilines, which were isolated from the hydrolysates by steam distillation/solvent extraction. Quantitative determinations were done by reversephase high performance liquid chromatography without derivatization. Anilines in subnanogram quantities were detected with an electrochemical detector. Recovery data were obtained from egg plant samples fortified with fluoroimide, aniliazine, linuron, alachlor or iprodione at 0.4ppm. Average recoveries for fluoroimide, anilazine, linuron, alachlor and iprodione were 100, 85, 68, 27 and 89%, respectively.

ガスクロマトグラフィーによる残留農薬の簡易分析法のための精製法として, 活性炭-フロリジルのミニカラムの有用性について検討した. 試料をメタノールで抽出し, 抽出物をトルエンへ転溶させ, トルエン溶液をミニカラムを用いて精製し, ガスクロマトグラフ (ECDおよびFTD) で定量した.25農薬, 15作物について回収率試験を行なった結果, dimethoate および oxadixyl 以外の農薬はすべて90%以上の回収率を得た. 検出限界は有機リン剤が0.01～0.02ppm, カーバメイトおよび合成ピレスロイドが0.05ppm, 有機塩素剤が0.005ppmであった. 本法は簡便かつ迅速な方法であり, 多くの農薬のモニタリングに広く適用できると思われる.