1. Cholinesterase inhibiting and antiplasmodial steroidal alkaloids from Sarcococca hookeriana.
- Author
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Devkota KP, Lenta BN, Choudhary MI, Naz Q, Fekam FB, Rosenthal PJ, and Sewald N
- Subjects
- Animals, Butyrylcholinesterase metabolism, Drug Resistance, Magnetic Resonance Spectroscopy, Plasmodium falciparum drug effects, Pregnanes isolation & purification, Spectrophotometry, Infrared, Spectrophotometry, Ultraviolet, Alkaloids isolation & purification, Alkaloids pharmacology, Antimalarials, Buxaceae chemistry, Cholinesterase Inhibitors, Pregnanes pharmacology, Steroids isolation & purification, Steroids pharmacology
- Abstract
Bioguided phytochemical investigation of Sarcococca hookeriana with respect to the cholinesterase enzyme inhibitory assay yielded two new pregnane-type steriodal alkaloids hookerianamide H (1) and hookerianamide I (2), along with three known alkaloids N(a)-methylepipachysamine D (3), sarcovagine C (4) and dictyophlebine (5). Their structures were determined with the aid of extensive spectroscopic analysis. All compounds showed good inhibitory activities against the enzymes acetylcholinesterase (IC(50) 2.9-34.1 microM) and butyrylcholinesterase (IC(50) 0.3-3.6 microM). These compounds also showed moderate antiplasmodial activity (IC(50) 2.4-10.3 microM) against the Plasmodium falciparum chloroquine resistant W2 strain.
- Published
- 2007
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