1. AFM-based protein fishing in the pulsed electric field
- Author
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N. D. Ivanova, Yu. D. Ivanov, Alexander I. Archakov, A. T. Kopylov, Anna L. Kaysheva, S. G. Vesnin, Alexander A. Izotov, Kristina A. Malsagova, Vadim S. Ziborov, V. Yu. Tatur, and Tatyana O. Pleshakova
- Subjects
Range (particle radiation) ,Materials science ,Clinical Biochemistry ,technology, industry, and agriculture ,Analytical chemistry ,macromolecular substances ,Mass spectrometry ,Chip ,Biochemistry ,Highly oriented pyrolytic graphite ,Electric field ,Rise time ,Molecular Medicine ,Water injection (engine) ,Voltage - Abstract
A combination of (atomic force microscopy)-based fishing (AFM-fishing) and mass spectrometry allows to capture protein molecules from solutions, concentrate and visualize them on an atomically flat surface of the AFM chip and identify by subsequent mass spectrometric analysis. In order to increase the AFM-fishing efficiency we have applied pulsed voltage with the rise time of the front of about 1 ns to the AFM chip. The AFM-chip was made using a conductive material, highly oriented pyrolytic graphite (HOPG). The increased efficiency of AFM-fishing has been demonstrated using detection of cytochrome b 5 protein. Selection of the stimulating pulse with a rise time of 1 ns, corresponding to the GHz frequency range, by the effect of intrinsic emission from water observed in this frequency range during water injection into the cell.
- Published
- 2015