1. AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging
- Author
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Wen-jun Lan, Can Huang, Xiaomei Liu, Qin Chen, Chun Zhang, and Feifei Wang
- Subjects
0301 basic medicine ,Near-Infrared Fluorescence Imaging ,Infrared Rays ,Genetic Vectors ,Biophysics ,Mice, Nude ,Biology ,Mesenchymal Stem Cell Transplantation ,Transfection ,Site-specific integration ,medicine.disease_cause ,Biochemistry ,Cell Line ,MSC ,Mice ,03 medical and health sciences ,Adeno-associated virus ,In vivo ,medicine ,Fluorescence microscope ,Animals ,Humans ,Molecular Biology ,Research Articles ,iRFP ,Optical Imaging ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Cell Biology ,Dependovirus ,Fluorescence ,Molecular biology ,Luminescent Proteins ,030104 developmental biology ,Microscopy, Fluorescence ,Cell Tracking ,Apoptosis ,Female ,in vivo imaging ,Preclinical imaging ,Research Article - Abstract
Near-IR fluorescence (NIRF) imaging is a new technology using IR fluorescent protein (iRFP) gene labelling and is potentially useful for in vivo applications. In the present study, we expressed iRFP and the TNF-related apoptosis inducing ligand gene in mesenchymal stem cells (MSCs) using adeno-associated virus (AAV) and showed that iRFP-labelled MSCs can be detected by fluorescence microscopy. We injected mice with MSCs labelled with AAV-iRFP, which we were then able to detect by whole-animal NIRF imaging. Our technique provides a visualizable, convenient and sensitive platform for research on tracking the fate of transplanted MSC cells in vivo.
- Published
- 2017
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