1. Engagement of CD160 receptor by HLA-C is a triggering mechanism used by circulating natural killer (NK) cells to mediate cytotoxicity
- Author
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Anne Marie-Cardine, Aliz Barakonyi, Julie Tabiasco, Philippe Le Bouteiller, Jérôme Giustiniani, Françoise Lenfant, Ivan Hilgert, Armand Bensussan, Magali Rabot, Fathia Mami-Chouaib, Laurence Boumsell, and Maryse Aguerre-Girr
- Subjects
Cytotoxicity, Immunologic ,HLA-C Antigens ,GPI-Linked Proteins ,Interleukin 21 ,Receptors, KIR ,Antigens, CD ,MHC class I ,Humans ,Cytotoxic T cell ,Lectins, C-Type ,Receptors, Immunologic ,Receptor ,Multidisciplinary ,Lymphokine-activated killer cell ,biology ,Chemistry ,Membrane Proteins ,Biological Sciences ,Cell biology ,Killer Cells, Natural ,Receptors, KIR2DL3 ,biology.protein ,Interleukin 12 ,Interleukin-2 ,NK Cell Lectin-Like Receptor Subfamily D ,K562 cells - Abstract
Circulating human natural killer (NK) lymphocytes have been functionally defined by their ability to exert cytotoxic activity against MHC class I-negative target cell lines, including K562. Therefore, it was proposed that NK cells recognized the “missing self.” We show here that the Ig-like CD160 receptor expressed by circulating CD56dim+NK cells or IL-2-deprived NK cell lines is mainly involved in their cytotoxic activity against K562 target cells. Further, we report that HLA-C molecules that are constitutively expressed by K562 trigger NK cell lysis through CD160 receptor engagement. In addition, we demonstrate, with recombinant soluble HLA-Cw3 and CD160 proteins, direct interaction of these molecules. We also find that CD158b inhibitory receptors partially interfere with CD160-mediated cytotoxicity, whereas CD94/CD159a and CD85j have no effect on engagement with their respective ligands. Thus, CD160/HLA-C interaction constitutes a unique pathway to trigger NK cell cytotoxic activity.
- Published
- 2002