1. The impact of vitamin D3 intake on inflammatory markers in multiple sclerosis patients and their first-degree relatives.
- Author
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Hashemi R, Hosseini-Asl SS, Arefhosseini SR, and Morshedi M
- Subjects
- Adult, Cholecalciferol administration & dosage, Cholecalciferol blood, Enzyme-Linked Immunosorbent Assay, Female, Healthy Volunteers, Humans, Inflammation blood, Male, Multiple Sclerosis blood, RNA, Messenger genetics, RNA, Messenger metabolism, Real-Time Polymerase Chain Reaction, Treatment Outcome, Up-Regulation genetics, Young Adult, Cholecalciferol therapeutic use, Family, Interleukins blood, Multiple Sclerosis drug therapy, Transforming Growth Factor beta1 blood
- Abstract
Background & Aims: In our previous study, a Seesaw model was proposed for the fluctuation of crucial anti- (IL-10) and pro-inflammatory (Il-6 & IL-17A) cytokines through vitamin D3. In this paper, however, it is intended to extend the mentioned model by assessing the expression mRNA levels of IL-27 and TGF-β1 as well as the changes of plasma levels of IL-27, TGF-β1, IL-17A, IL-10, and IL-6 after treatment by vitamin D3., Method: Venous blood samples were drawn from Healthy Participants (HP, n = 25) and First-Degree Relative Participants (FDRP, n = 25) as control groups and Multiple Sclerosis Participants (MSP, n = 25) before and after eight weeks of supplementation with 50000 IU vitamin D3. The mRNA expression and plasma concentrations were gauged by using Real-Time PCR and ELISA assay, respectively., Results: The mRNA surfaces of IL-27, as well as TGF-β1, were up-regulated. However, the plasma levels of TGF-β1, IL-17A, and IL-6 were significantly different among the three groups. In addition, the plasma levels of IL-27, TGF-β1, IL-10, IL-17A, and IL-6 significantly changed following the administration of vitamin D3., Conclusion: The findings of this paper illustrate that anti-inflammatory cytokines could have a key role in immunomodulatory functions due to their anti-inflammatory functions. To conclude, this might contribute to preventing the pathophysiological process of MS. Also, the proposed model could be used as a preventive way on disposed people to multiple sclerosis, particularly in first degree relatives of these patients., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2020
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