1. An in vitro model of intestinal infection reveals a developmentally regulated transcriptome of Toxoplasma sporozoites and a NF-κB-like signature in infected host cells.
- Author
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Guiton PS, Sagawa JM, Fritz HM, and Boothroyd JC
- Subjects
- Animals, Cell Line, Humans, Intestinal Mucosa metabolism, Protozoan Proteins metabolism, Rats, Toxoplasmosis metabolism, Transcriptome genetics, Tumor Necrosis Factor-alpha metabolism, NF-kappa B metabolism, Sporozoites metabolism, Toxoplasma genetics
- Abstract
Toxoplasmosis is a zoonotic infection affecting approximately 30% of the world's human population. After sexual reproduction in the definitive feline host, Toxoplasma oocysts, each containing 8 sporozoites, are shed into the environment where they can go on to infect humans and other warm-blooded intermediate hosts. Here, we use an in vitro model to assess host transcriptomic changes that occur in the earliest stages of such infections. We show that infection of rat intestinal epithelial cells with mature sporozoites primarily results in higher expression of genes associated with Tumor Necrosis Factor alpha (TNFα) signaling via NF-κB. Furthermore, we find that, consistent with their biology, these mature, invaded sporozoites display a transcriptome intermediate between the previously reported day 10 oocysts and that of their tachyzoite counterparts. Thus, this study uncovers novel host and pathogen factors that may be critical for the establishment of a successful intracellular niche following sporozoite-initiated infection.
- Published
- 2017
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