1. Crif1 Deficiency Reduces Adipose OXPHOS Capacity and Triggers Inflammation and Insulin Resistance in Mice
- Author
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Jongkyeong Chung, Soung Jung Kim, Jin-Man Kim, Min Jeong Ryu, Seong Eun Lee, Yong Kyung Kim, Surendar Tadi, Sang-Hee Lee, Koon Soon Kim, Jung Uee Lee, Chul-Ho Lee, Saet Byel Jung, Young Suk Jo, Ki Cheol Park, Hyun-Jin Kim, Young-Yun Kong, Min Hee Lee, Hyo Kyun Chung, Gi Ryang Kweon, Minho Shong, and Min Jeong Choi
- Subjects
Cancer Research ,medicine.medical_specialty ,lcsh:QH426-470 ,medicine.medical_treatment ,Adipose tissue ,Inflammation ,Cell Cycle Proteins ,Haploinsufficiency ,Mitochondrion ,Biology ,Systemic inflammation ,DNA, Mitochondrial ,Oxidative Phosphorylation ,Mice ,Insulin resistance ,Internal medicine ,Brown adipose tissue ,Genetics ,medicine ,Adipocytes ,Animals ,Insulin ,Obesity ,Molecular Biology ,Genetics (clinical) ,Ecology, Evolution, Behavior and Systematics ,Macrophages ,medicine.disease ,Mitochondria ,lcsh:Genetics ,Endocrinology ,medicine.anatomical_structure ,Adipose Tissue ,Medicine ,Tumor necrosis factor alpha ,medicine.symptom ,Insulin Resistance ,Research Article - Abstract
Impaired mitochondrial oxidative phosphorylation (OXPHOS) has been proposed as an etiological mechanism underlying insulin resistance. However, the initiating organ of OXPHOS dysfunction during the development of systemic insulin resistance has yet to be identified. To determine whether adipose OXPHOS deficiency plays an etiological role in systemic insulin resistance, the metabolic phenotype of mice with OXPHOS–deficient adipose tissue was examined. Crif1 is a protein required for the intramitochondrial production of mtDNA–encoded OXPHOS subunits; therefore, Crif1 haploinsufficient deficiency in mice results in a mild, but specific, failure of OXPHOS capacity in vivo. Although adipose-specific Crif1-haploinsufficient mice showed normal growth and development, they became insulin-resistant. Crif1-silenced adipocytes showed higher expression of chemokines, the expression of which is dependent upon stress kinases and antioxidant. Accordingly, examination of adipose tissue from Crif1-haploinsufficient mice revealed increased secretion of MCP1 and TNFα, as well as marked infiltration by macrophages. These findings indicate that the OXPHOS status of adipose tissue determines its metabolic and inflammatory responses, and may cause systemic inflammation and insulin resistance., Author Summary Type 2 diabetes is one of the most challenging health problems in the 21st century. Although insulin resistance is regarded as a fundamental defect that precedes the development of type 2 diabetes, the nature and cause of insulin resistance remain unknown. Adipose tissue is an important organ that determines whole-body energy metabolism, and its dysfunction is a critical element in the development of systemic insulin resistance. Adipose mitochondrial function is suppressed in the insulin-resistant state, and increased adipose mitochondrial biogenesis is associated with the reversal of insulin resistance by a PPARγ agonist. However, despite these important observations, little is known about how mitochondrial respiratory dysfunction in white adipose tissue (WAT) causes insulin resistance. To determine whether adipose deficiency of mitochondrial respiratory capacity plays an etiological role in systemic insulin resistance, the metabolic phenotype of mice with mitochondrial OXPHOS (oxidative phosphorylation)–deficient adipose tissue was examined. Crif1 is a protein required for the translation of mtDNA–encoded OXPHOS subunits. Interestingly, mice haploinsufficient for Crif1 in adipose tissue showed reduced OXPHOS capacity and developed marked insulin resistance.
- Published
- 2013