1. CD19(+) B Cells Confer Protection against Experimental Cerebral Malaria in Semi-Immune Rodent Model
- Author
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Kenji Hirayama, Katsuyuki Yui, Masachika Senba, Lam Quoc Bao, Kiri Honma, Nguyen Tien Huy, Mihoko Kikuchi, Mohammed Nasir Shuaibu, and Tetsuo Yanagi
- Subjects
Adoptive cell transfer ,B Cells ,Mouse ,Plasmodium berghei ,lcsh:Medicine ,Antibodies, Protozoan ,Parasitemia ,Mice ,lcsh:Science ,Disease Resistance ,B-Lymphocytes ,Multidisciplinary ,Statistics ,Animal Models ,Adoptive Transfer ,Interleukin-10 ,Infectious Diseases ,medicine.anatomical_structure ,Cerebral Malaria ,Cytokines ,Medicine ,Disease Susceptibility ,Antibody ,Research Article ,Histology ,Immune Cells ,Immunology ,Antigens, CD19 ,Malaria, Cerebral ,Spleen ,Immunopathology ,Biostatistics ,Biology ,CD5 Antigens ,Interferon-gamma ,Model Organisms ,Immune system ,Species Specificity ,Antigen ,Parasitic Diseases ,medicine ,Animals ,lcsh:R ,Immunity ,Tropical Diseases (Non-Neglected) ,medicine.disease ,biology.organism_classification ,Malaria ,Mice, Inbred C57BL ,Disease Models, Animal ,Immune System ,Immunoglobulin G ,biology.protein ,lcsh:Q ,Clinical Immunology ,Mathematics - Abstract
In African endemic area, adults are less vulnerable to cerebral malaria than children probably because of acquired partial immunity or semi-immune status. Here, we developed an experimental cerebral malaria (ECM) model for semi-immune mice. C57BL/6 (B6) mice underwent one, two and three cycles of infection and radical treatment (1-cure, 2-cure and 3-cure, respectively) before being finally challenged with 104 Plasmodium berghei ANKA without treatment. Our results showed that 100% of naïve (0-cure), 67% of 1-cure, 37% of 2-cure and none of 3-cure mice succumbed to ECM within 10 days post challenge infection. In the protected 3-cure mice, significantly higher levels of plasma IL-10 and lower levels of IFN-γ than the others on day 7 post challenge infection were observed. Major increased lymphocyte subset of IL-10 positive cells in 3-cure mice was CD5(-)CD19(+) B cells. Passive transfer of splenic CD19(+) cells from 3-cure mice protected naïve mice from ECM. Additionally, aged 3-cure mice were also protected from ECM 12 and 20 months after the last challenge infection. In conclusion, mice became completely resistant to ECM after three exposures to malaria. CD19(+) B cells are determinants in protective mechanism of semi-immune mice against ECM possibly via modulatory IL-10 for pathogenic IFN-γ production., PLoS ONE, 8(5), e64836; 2013
- Published
- 2013