1. Evaluation of the Protective Immunity of a Novel Subunit Fusion Vaccine in a Murine Model of Systemic MRSA Infection
- Author
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Chang Zhi Cai, Dong–Shui Lu, Yan Dong Dong, Yi Wu, Qian Fei Zuo, Qiang Feng, Quan Ming Zou, Ying Guo, Liu Yang Yang, Hao Zeng, and Jiang Gu
- Subjects
Male ,Methicillin-Resistant Staphylococcus aureus ,Recombinant Fusion Proteins ,T-Lymphocytes ,lcsh:Medicine ,Human leukocyte antigen ,Biology ,medicine.disease_cause ,Staphylococcal infections ,Microbiology ,Mice ,Immune system ,Antigen ,Phagocytosis ,Immunity ,medicine ,Animals ,Cloning, Molecular ,lcsh:Science ,Antigens, Bacterial ,Mice, Inbred BALB C ,Multidisciplinary ,lcsh:R ,Staphylococcal Vaccines ,Opsonin Proteins ,Staphylococcal Infections ,medicine.disease ,Methicillin-resistant Staphylococcus aureus ,Virology ,Antibodies, Neutralizing ,Disease Models, Animal ,Staphylococcus aureus ,Immunoglobulin G ,Antibody Formation ,Vaccines, Subunit ,biology.protein ,lcsh:Q ,Immunization ,Antibody ,Chemokines ,Spleen ,Research Article - Abstract
Staphylococcus aureus is a common commensal organism in humans and a major cause of bacteremia and hospital acquired infection. Because of the spread of strains resistant to antibiotics, these infections are becoming more difficult to treat. Therefore, exploration of anti-staphylococcal vaccines is currently a high priority. Iron surface determinant B (IsdB) is an iron-regulated cell wall-anchored surface protein of S. aureus. Alpha-toxin (Hla) is a secreted cytolytic pore-forming toxin. Previous studies reported that immunization with IsdB or Hla protected animals against S. aureus infection. To develop a broadly protective vaccine, we constructed chimeric vaccines based on IsdB and Hla. Immunization with the chimeric bivalent vaccine induced strong antibody and T cell responses. When the protective efficacy of the chimeric bivalent vaccine was compared to that of individual proteins in a murine model of systemic S. aureus infection, the bivalent vaccine showed a stronger protective immune response than the individual proteins (IsdB or Hla). Based on the results presented here, the chimeric bivalent vaccine affords higher levels of protection against S. aureus and has potential as a more effective candidate vaccine.
- Published
- 2013