1. Deep brain stimulation restores the glutamatergic and GABAergic synaptic transmission and plasticity to normal levels in kindled rats
- Author
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Amir Shojaei, Saeed Semnanian, Samireh Ghafouri, Azam Asgari, Azin Ebrahim Amini, Yaghoub Fathollahi, and Javad Mirnajafi-Zadeh
- Subjects
0301 basic medicine ,Male ,Physiology ,Deep Brain Stimulation ,Long-Term Potentiation ,Gene Expression ,Hippocampal formation ,Nervous System ,Biochemistry ,Hippocampus ,Synaptic Transmission ,0302 clinical medicine ,Glutamates ,Animal Cells ,LTP induction ,Medicine and Health Sciences ,GABAergic Neurons ,Neurons ,Multidisciplinary ,Neuronal Plasticity ,Chemistry ,musculoskeletal, neural, and ocular physiology ,Drugs ,Long-term potentiation ,Neurochemistry ,Neurotransmitters ,Electrophysiology ,Receptors, Glutamate ,Excitatory postsynaptic potential ,Medicine ,Anticonvulsants ,Anatomy ,Cellular Types ,Research Article ,Science ,Neurophysiology ,Surgical and Invasive Medical Procedures ,Neurotransmission ,Inhibitory postsynaptic potential ,03 medical and health sciences ,Glutamatergic ,Developmental Neuroscience ,Genetics ,Kindling, Neurologic ,Animals ,Rats, Wistar ,Pharmacology ,Functional Electrical Stimulation ,Biology and Life Sciences ,Excitatory Postsynaptic Potentials ,Cell Biology ,Gamma-Aminobutyric Acid ,030104 developmental biology ,nervous system ,Gene Expression Regulation ,Cellular Neuroscience ,Synaptic plasticity ,Synapses ,Neuroscience ,030217 neurology & neurosurgery ,Synaptic Plasticity - Abstract
Background The precise effect of low frequency stimulation (LFS) as a newly postulated, anticonvulsant therapeutic approach on seizure-induced changes in synaptic transmission has not been completely determined. Hypothesis In this study, the LFS effect on impaired, synaptic plasticity in kindled rats was investigated. Methods Hippocampal kindled rats received LFS (4 trials consisting of one train of 200 monophasic square waves, 0.1 ms pulse duration, 1 Hz) on four occasions. LTP induction was evaluated using whole-cell recordings of evoked excitatory and inhibitory post-synaptic potentials (EPSPs and IPSPs respectively) in CA1 neurons in hippocampal slices. In addition, the hippocampal excitatory and inhibitory post-synaptic currents (EPSCs and IPSCs), and the gene expression of NR2A, GluR2 and γ2 were evaluated. Results LTP induction was attenuated in excitatory and inhibitory synapses in hippocampal slices of kindled rats. When LFS was applied in kindled animals, LTP was induced in EPSPs and IPSPs. Moreover, LFS increased and decreased the threshold intensities of EPSCs and IPSCs respectively. In kindled animals, NR2A gene expression increased, while γ2 gene expression decreased. GluR2 gene expression did not significantly change. Applying LFS in kindled animals mitigated these changes: No significant differences were observed in NR2A, γ2 and GluR2 gene expression in the kindled+LFS and control groups. Conclusion The application of LFS in kindled animals restored LTP induction in both EPSPs and IPSPs, and returned the threshold intensity for induction of EPSCs, IPSCs and gene expression to similar levels as controls.
- Published
- 2019