Search

Your search keyword '"Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings]"' showing total 2 results
Start Over Descriptor "Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings]" Publisher public library of science (plos)
2 results on '"Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings]"'

1. Allergens Induce the Release of Lactoferrin by Neutrophils from Asthmatic Patients

Author

Inmaculada Ventura, Rocío Aroca, M Prados, Javier Monteseirín, Bobadilla P, Antonio Vega-Rioja, Ricardo Palacios, Cristina Chamorro, Lourdes Fernández-Delgado, David Rodriguez, [Fernández-Delgado,L, Vega-Rioja,A, Ventura,I, Chamorro,C, Aroca,R, Prados,M, Monteseirín,J] Unidad de Gestión Clínica de Alergia Intercentros, Hospital Universitario Virgen Macarena, Sevilla, Spain. [Monteseirín,J] Departamento de Medicina. Facultad de Medicina. Universidad de Sevilla, Sevilla, Spain. [Bobadilla,P] Servicio de Alergia, Hospital Infanta Elena, Badajoz, Spain. [Rodríguez,D, Palacios,R] Laboratorios Diater, Madrid, Spain., and This work was supported by the FIS-Thematic Networks and Co-Operative Research Centres RIRAAF (RD07-0064) and Fundación Alergol, Spain. Javier Monteseirín is under the Programa de Intensificación de la Actividad Investigadora del Sistema Nacional de Salud.

Subjects
Male, Allergy, Galectina 3, Neutrophils, Galectin 3, lcsh:Medicine, medicine.disease_cause, Immunoglobulin E, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Allergen, Alérgenos, Phenomena and Processes::Biological Phenomena::Biological Processes [Medical Subject Headings], Receptor, lcsh:Science, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E [Medical Subject Headings], Multidisciplinary, biology, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Lectins::Galectins::Galectin 3 [Medical Subject Headings], Lactoferrin, food and beverages, Middle Aged, Female, medicine.symptom, Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Immunologic Factors::Immunosuppressive Agents [Medical Subject Headings], Research Article, Adult, Adolescent, Inflammation, Diseases::Respiratory Tract Diseases::Lung Diseases::Lung Diseases, Obstructive::Asthma [Medical Subject Headings], Young Adult, medicine, Humans, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies, Monoclonal [Medical Subject Headings], Asma, Asthma, Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Peptide Hydrolases::Endopeptidases::Serine Endopeptidases::Lactoferrin [Medical Subject Headings], business.industry, Receptors, IgE, lcsh:R, Allergens, medicine.disease, Inmunosupresores, In vitro, Case-Control Studies, Anticuerpos monoclonales, Immunology, biology.protein, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Fc::Receptors, IgE [Medical Subject Headings], lcsh:Q, Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings], Anatomy::Cells::Blood Cells::Leukocytes::Granulocytes::Neutrophils [Medical Subject Headings], business, Chemicals and Drugs::Biological Factors::Inflammation Mediators [Medical Subject Headings]
Abstract

Journal Article; BACKGROUND Despite the evidence that Lactoferrin (Lf) is involved in allergic asthma processes, it is unknown whether neutrophils can be one of the main cellular sources of this key inflammatory mediator directly in response of an IgE mediated stimulus. The present study was undertaken to analyze this question. METHODS Neutrophils from healthy subjects (n = 34) and neutrophils from allergic asthmatic patients (n = 102) were challenged in vitro with specific allergens to which the patients were sensitized, PAF, or agonist mAbs against IgE-receptors, and the levels of Lf were measured in the culture supernatant. The levels of serum IgE together with the severity of symptoms were also analyzed. RESULTS Lf was released into the culture supernatant of neutrophils from allergic asthmatic patients in response to allergens and PAF. This response was highly allergen-specific, and did not happen in neutrophils from healthy donors. Allergen effect was mimicked by Abs against FcεRI and galectin-3 but not by FcεRII. The levels of released Lf correlated well with the levels of serum specific IgE and severity of asthma symptoms. These observations represent a novel view of neutrophils as an important source of Lf in allergic asthma. Importantly, the levels of released Lf by neutrophils could therefore be used to evaluate disease severity in allergic asthmatic patients. Yes

Published
2015

2. Antigenic Proteins Involved in Occupational Rhinitis and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon)

Author

Inmaculada Doña, Arantxa Palacín, Araceli Díaz-Perales, Ana Aranda, Cristina Gomez-Casado, Paloma Campo, Miguel Blanca, Luisa Galindo, [Aranda,A] Allergy Research Laboratory, Carlos Haya Hospital, Málaga, Spain. [Campo,P, Doña,I, Galindo,L, Blanca,M] Unidad de Gestión Clínica Allergy, Carlos Haya Hospital, Málaga, Spain. [Palacin,A, Gomez-Casado,C, Díaz-Perales,A] Biotechnology Department, Center for Plant Biotechnology and Genomics, Pozuelo de Alarcon, Madrid, Spain., Ministry of Health grant FIS/PI071175, Andalusian Health Division grants PI-0182/2008, grant from the Spanish Society of Allergy, Ministry of Science and Innovation-DGI, Spain (grant BIO2009-07050), and and Thematic Network FIS-RIRAAF (RD07/0064).

Subjects
Male, Triplochiton scleroxylon, Allergy, Pulmonology, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Globulins::Serum Globulins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E [Medical Subject Headings], lcsh:Medicine, Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings], Plant Science, Biochemistry, Trees, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Diseases::Occupational Diseases [Medical Subject Headings], 0302 clinical medicine, Alérgenos, Anatomy::Plant Structures::Xylem::Wood [Medical Subject Headings], Medicine, lcsh:Science, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Plant Proteins [Medical Subject Headings], Malvaceae, Rinitis, Plant Proteins, Rhinitis, 0303 health sciences, Multidisciplinary, Allergy and Hypersensitivity, Middle Aged, Plants, Occupational and Industrial Health, Diseases::Respiratory Tract Diseases::Nose Diseases::Rhinitis [Medical Subject Headings], Wood, Madera, 3. Good health, Occupational Diseases, Electroforesis en gel de poliacrilamida, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Immunologic Techniques::Immunoassay::Immunoenzyme Techniques::Enzyme-Linked Immunosorbent Assay [Medical Subject Headings], Named Groups::Persons::Age Groups::Adolescent [Medical Subject Headings], visual_art, Plant biochemistry, Diseases::Respiratory Tract Diseases::Respiratory Hypersensitivity::Asthma [Medical Subject Headings], visual_art.visual_art_medium, Electrophoresis, Polyacrylamide Gel, Female, Exposición Profesional, Public Health, Occupational exposure, Enfermedades profesionales, Occupational asthma, Research Article, Adult, Named Groups::Persons::Age Groups::Adult::Young Adult [Medical Subject Headings], Adolescent, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Electrochemical Techniques::Electrophoresis::Electrophoresis, Polyacrylamide Gel [Medical Subject Headings], Immunology, Check Tags::Male [Medical Subject Headings], Immunoglobulins, Enzyme-Linked Immunosorbent Assay, Environmental and Occupational Lung Diseases, complex mixtures, Young Adult, 03 medical and health sciences, Occupational Exposure, Named Groups::Persons::Age Groups::Adult [Medical Subject Headings], Humans, Biology, Asma, Skin Tests, 030304 developmental biology, Asthma, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Globulins::Serum Globulins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin G [Medical Subject Headings], Enzimoinmunoanálisis por adsorción, business.industry, lcsh:R, Proteins, Health Care::Environment and Public Health::Public Health::Environmental Pollution::Environmental Exposure::Occupational Exposure [Medical Subject Headings], Allergens, Immunoglobulin E, medicine.disease, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Immunologic Tests::Skin Tests [Medical Subject Headings], respiratory tract diseases, Check Tags::Female [Medical Subject Headings], 030228 respiratory system, Immunoglobulin G, lcsh:Q, Clinical Immunology, Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings], Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Malvaceae [Medical Subject Headings], business
Abstract

Journal Article; Research Support, Non-U.S. Gov't; BACKGROUND Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated. OBJECTIVE To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma MATERIALS AND METHODS An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies. RESULTS Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results