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Your search keyword '"Denis V. Kurek"' showing total 2 results
Start Over Author "Denis V. Kurek" Publisher public library of science (plos)
2 results on '"Denis V. Kurek"'

1. Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles.

Author

Denis S Grouzdev, Marina V Dziuba, Denis V Kurek, Alexander I Ovchinnikov, Nadezhda A Zhigalova, Boris B Kuznetsov, and Konstantin G Skryabin

Subjects
Medicine, Science
Abstract

In this study, the optimized method for designing IgG-binding magnetosomes based on integration of IgG-binding fusion proteins into magnetosome membrane in vitro is presented. Fusion proteins Mbb and Mistbb consisting of magnetosome membrane protein MamC and membrane associating protein Mistic from Bacillus subtilis as anchors and BB-domains of Staphylococcus aureus protein A as IgG-binding region were used. With Response Surface Methodology (RSM) the highest level of proteins integration into magnetosome membrane was achieved under the following parameters: pH 8.78, without adding NaCl and 55 s of vortexing for Mbb; pH 9.48, 323 mM NaCl and 55 s of vortexing for Mistbb. Modified magnetosomes with Mbb and Mistbb displayed on their surface demonstrated comparable levels of IgG-binding activity, suggesting that both proteins could be efficiently used as anchor molecules. We also demonstrated that such modified magnetosomes are stable in PBS buffer during at least two weeks. IgG-binding magnetosomes obtained by this approach could serve as a multifunctional platform for displaying various types of antibodies.

Published
2014
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2. Optimized method for preparation of IgG-binding bacterial magnetic nanoparticles

Author

A. I. Ovchinnikov, Nadezhda Zhigalova, Boris B. Kuznetsov, Denis S. Grouzdev, Marina V. Dziuba, Denis V. Kurek, and Konstantin G. Skryabin

Subjects
Staphylococcus aureus, Applied Microbiology, Science, Magnetosome, Biophysics, Bioengineering, Plasma protein binding, Bacillus subtilis, Protein Engineering, Biochemistry, Microbiology, Antibodies, Biomaterials, Bacterial Proteins, Staphylococcus aureus protein A, Macromolecular Engineering, Magnetite Nanoparticles, Staphylococcal Protein A, Immune System Proteins, Multidisciplinary, biology, Chemistry, Conjugated Proteins, Biology and Life Sciences, Proteins, Membrane Proteins, Lipid bilayer fusion, biology.organism_classification, Fusion protein, Membrane, Membrane protein, Synthetic Bioengineering, Immunoglobulin G, Bionanotechnology, Medicine, Magnetosomes, Research Article, Biotechnology, Protein Binding
Abstract

In this study, the optimized method for designing IgG-binding magnetosomes based on integration of IgG-binding fusion proteins into magnetosome membrane in vitro is presented. Fusion proteins Mbb and Mistbb consisting of magnetosome membrane protein MamC and membrane associating protein Mistic from Bacillus subtilis as anchors and BB-domains of Staphylococcus aureus protein A as IgG-binding region were used. With Response Surface Methodology (RSM) the highest level of proteins integration into magnetosome membrane was achieved under the following parameters: pH 8.78, without adding NaCl and 55 s of vortexing for Mbb; pH 9.48, 323 mM NaCl and 55 s of vortexing for Mistbb. Modified magnetosomes with Mbb and Mistbb displayed on their surface demonstrated comparable levels of IgG-binding activity, suggesting that both proteins could be efficiently used as anchor molecules. We also demonstrated that such modified magnetosomes are stable in PBS buffer during at least two weeks. IgG-binding magnetosomes obtained by this approach could serve as a multifunctional platform for displaying various types of antibodies.

Published
2014

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