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Start Over Author "Eliana Ruberto" Publisher public library of science (plos)
2 results on '"Eliana Ruberto"'

1. Disrupted intestinal microbiota and intestinal inflammation in children with cystic fibrosis and its restoration with Lactobacillus GG: a randomised clinical trial.

Author

Eugenia Bruzzese, Maria Luisa Callegari, Valeria Raia, Sara Viscovo, Riccardo Scotto, Susanna Ferrari, Lorenzo Morelli, Vittoria Buccigrossi, Andrea Lo Vecchio, Eliana Ruberto, and Alfredo Guarino

Subjects
Medicine, Science
Abstract

Intestinal inflammation is a hallmark of cystic fibrosis (CF). Administration of probiotics can reduce intestinal inflammation and the incidence of pulmonary exacerbations. We investigated the composition of intestinal microbiota in children with CF and analyzed its relationship with intestinal inflammation. We also investigated the microflora structure before and after Lactobacillus GG (LGG) administration in children with CF with and without antibiotic treatment.The intestinal microbiota were analyzed by denaturing gradient gel electrophoresis (DGGE), real-time polymerase chain reaction (RT-PCR), and fluorescence in situ hybridization (FISH). Intestinal inflammation was assessed by measuring fecal calprotectin (CLP) and rectal nitric oxide (rNO) production in children with CF as compared with healthy controls. We then carried out a small double-blind randomized clinical trial with LGG.Twenty-two children with CF children were enrolled in the study (median age, 7 years; range, 2-9 years). Fecal CLP and rNO levels were higher in children with CF than in healthy controls (184±146 µg/g vs. 52±46 µg/g; 18±15 vs. 2.6±1.2 µmol/L NO2 (-), respectively; P

Published
2014
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2. Disrupted intestinal microbiota and intestinal inflammation in children with cystic fibrosis and its restoration with Lactobacillus GG: a randomised clinical trial

Author

Andrea Lo Vecchio, Valeria Raia, Alfredo Guarino, Eliana Ruberto, Vittoria Buccigrossi, Maria Luisa Callegari, Lorenzo Morelli, Riccardo Scotto, Susanna Ferrari, Eugenia Bruzzese, Sara Viscovo, Bruzzese, Eugenia, Callegari, Ml, Raia, Valeria, Viscovo, S, Scotto, R, Ferrari, S, Morelli, L, Buccigrossi, Vittoria, LO VECCHIO, Andrea, Ruberto, E, and Guarino, Alfredo

Subjects
Male, Lactobacillus GG, Cystic Fibrosis, Antibiotics, Faecalibacterium prausnitzii, lcsh:Medicine, Gut flora, Pediatrics, Cystic fibrosis, Inflammatory bowel disease, antibiotics, Feces, fluids and secretions, Autosomal Recessive, Child, bacteria, lcsh:Science, Multidisciplinary, Ecology, biology, Microbiota, Biota, Intestines, Community Ecology, Child, Preschool, Settore AGR/16 - MICROBIOLOGIA AGRARIA, child health, Medicine, Pediatric Gastroenterology, Female, Research Article, Ecological Metrics, medicine.drug_class, Gastroenterology and Hepatology, Microbiology, Double-Blind Method, medicine, Humans, Biology, Community Structure, Inflammation, Clinical Genetics, lcsh:R, Immunity, medicine.disease, biology.organism_classification, Lactobacillus, probiotics, Case-Control Studies, Immunology, lcsh:Q, Species Richness, Bacteroides, Calprotectin
Abstract

Background & Aims Intestinal inflammation is a hallmark of cystic fibrosis (CF). Administration of probiotics can reduce intestinal inflammation and the incidence of pulmonary exacerbations. We investigated the composition of intestinal microbiota in children with CF and analyzed its relationship with intestinal inflammation. We also investigated the microflora structure before and after Lactobacillus GG (LGG) administration in children with CF with and without antibiotic treatment. Methods The intestinal microbiota were analyzed by denaturing gradient gel electrophoresis (DGGE), real-time polymerase chain reaction (RT-PCR), and fluorescence in situ hybridization (FISH). Intestinal inflammation was assessed by measuring fecal calprotectin (CLP) and rectal nitric oxide (rNO) production in children with CF as compared with healthy controls. We then carried out a small double-blind randomized clinical trial with LGG. Results Twenty-two children with CF children were enrolled in the study (median age, 7 years; range, 2–9 years). Fecal CLP and rNO levels were higher in children with CF than in healthy controls (184±146 µg/g vs. 52±46 µg/g; 18±15 vs. 2.6±1.2 µmol/L NO2−, respectively; P

Published
2014

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