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1. Genomic analysis of Shiga toxin-producing Escherichia coli from patients and asymptomatic food handlers in Japan.

Author

Hiroaki Baba, Hajime Kanamori, Hayami Kudo, Yasutoshi Kuroki, Seiya Higashi, Kentaro Oka, Motomichi Takahashi, Makiko Yoshida, Kengo Oshima, Tetsuji Aoyagi, Koichi Tokuda, and Mitsuo Kaku

Subjects
Medicine, Science
Abstract

Shiga toxin-producing Escherichia coli (STEC) can cause severe gastrointestinal disease and colonization among food handlers. In Japan, STEC infection is a notifiable disease, and food handlers are required to undergo routine stool examination for STEC. However, the molecular epidemiology of STEC is not entirely known. We investigated the genomic characteristics of STEC from patients and asymptomatic food handlers in Miyagi Prefecture, Japan. Whole-genome sequencing (WGS) was performed on 65 STEC isolates obtained from 38 patients and 27 food handlers by public health surveillance in Miyagi Prefecture between April 2016 and March 2017. Isolates of O157:H7 ST11 and O26:H11 ST21 were predominant (n = 19, 29%, respectively). Non-O157 isolates accounted for 69% (n = 45) of all isolates. Among 48 isolates with serotypes found in the patients (serotype O157:H7 and 5 non-O157 serotypes, O26:H11, O103:H2, O103:H8, O121:H19 and O145:H28), adhesion genes eae, tir, and espB, and type III secretion system genes espA, espJ, nleA, nleB, and nleC were detected in 41 to 47 isolates (85-98%), whereas isolates with other serotypes found only in food handlers were negative for all of these genes. Non-O157 isolates were especially prevalent among patients younger than 5 years old. Shiga-toxin gene stx1a, adhesion gene efa1, secretion system genes espF and cif, and fimbrial gene lpfA were significantly more frequent among non-O157 isolates from patients than among O157 isolates from patients. The most prevalent resistance genes among our STEC isolates were aminoglycoside resistance genes, followed by sulfamethoxazole/trimethoprim resistance genes. WGS revealed that 20 isolates were divided into 9 indistinguishable core genomes (

Published
2019
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2. Genomic analysis of Shiga toxin-producing Escherichia coli from patients and asymptomatic food handlers in Japan

Author

Hayami Kudo, Tetsuji Aoyagi, Hiroaki Baba, Koichi Tokuda, Mitsuo Kaku, Kentaro Oka, Motomichi Takahashi, Yasutoshi Kuroki, Makiko Yoshida, Seiya Higashi, Kengo Oshima, and Hajime Kanamori

Subjects
Male, Bacterial Diseases, 0301 basic medicine, Serotype, Food Handling, Secretion Systems, Pathology and Laboratory Medicine, medicine.disease_cause, Geographical Locations, fluids and secretions, Japan, Microbial Physiology, Medicine and Health Sciences, Bacterial Physiology, Child, Escherichia coli Infections, Phylogeny, Data Management, Aged, 80 and over, Escherichia Coli, Multidisciplinary, Shiga-Toxigenic Escherichia coli, Virulence, Incidence, Sulfamethoxazole, Phylogenetic Analysis, Genomics, Middle Aged, Bacterial Pathogens, Phylogenetics, Infectious Diseases, Experimental Organism Systems, Medical Microbiology, Child, Preschool, Prokaryotic Models, Medicine, Female, Pathogens, Research Article, medicine.drug, Adult, Escherichia, Computer and Information Sciences, Asia, Adolescent, Virulence Factors, Science, 030106 microbiology, Single-nucleotide polymorphism, Biology, Serogroup, Research and Analysis Methods, Microbiology, Young Adult, 03 medical and health sciences, Model Organisms, Antibiotic resistance, Enterobacteriaceae, Microbial Control, medicine, Animals, Humans, Evolutionary Systematics, Microbial Pathogens, Escherichia coli, Aged, Taxonomy, Pharmacology, Evolutionary Biology, Bacteria, Molecular epidemiology, Gut Bacteria, Organisms, Infant, Biology and Life Sciences, Bacteriology, 030104 developmental biology, Age Groups, People and Places, Hemolytic-Uremic Syndrome, Animal Studies, bacteria, Multilocus sequence typing, Population Groupings, Antimicrobial Resistance, Genome, Bacterial, Multilocus Sequence Typing
Abstract

Shiga toxin-producing Escherichia coli (STEC) can cause severe gastrointestinal disease and colonization among food handlers. In Japan, STEC infection is a notifiable disease, and food handlers are required to undergo routine stool examination for STEC. However, the molecular epidemiology of STEC is not entirely known. We investigated the genomic characteristics of STEC from patients and asymptomatic food handlers in Miyagi Prefecture, Japan. Whole-genome sequencing (WGS) was performed on 65 STEC isolates obtained from 38 patients and 27 food handlers by public health surveillance in Miyagi Prefecture between April 2016 and March 2017. Isolates of O157:H7 ST11 and O26:H11 ST21 were predominant (n = 19, 29%, respectively). Non-O157 isolates accounted for 69% (n = 45) of all isolates. Among 48 isolates with serotypes found in the patients (serotype O157:H7 and 5 non-O157 serotypes, O26:H11, O103:H2, O103:H8, O121:H19 and O145:H28), adhesion genes eae, tir, and espB, and type III secretion system genes espA, espJ, nleA, nleB, and nleC were detected in 41 to 47 isolates (85–98%), whereas isolates with other serotypes found only in food handlers were negative for all of these genes. Non-O157 isolates were especially prevalent among patients younger than 5 years old. Shiga-toxin gene stx1a, adhesion gene efa1, secretion system genes espF and cif, and fimbrial gene lpfA were significantly more frequent among non-O157 isolates from patients than among O157 isolates from patients. The most prevalent resistance genes among our STEC isolates were aminoglycoside resistance genes, followed by sulfamethoxazole/trimethoprim resistance genes. WGS revealed that 20 isolates were divided into 9 indistinguishable core genomes (

Published
2019
Full Text
View/download PDF

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