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2 results on '"Jacqueline Rosains"'

1. Genetic characterization of smg-8 mutants reveals no role in C. elegans nonsense mediated decay.

Author

Jacqueline Rosains and Susan E Mango

Subjects
Medicine, Science
Abstract

The nonsense mediated decay (NMD) pathway degrades mRNAs bearing premature translation termination codons. In mammals, SMG-8 has been implicated in the NMD pathway, in part by its association with SMG-1 kinase. Here we use four independent assays to show that C. elegans smg-8 is not required to degrade nonsense-containing mRNAs. We examine the genetic requirement for smg-8 to destabilize the endogenous, natural NMD targets produced by alternative splicing of rpl-7a and rpl-12. We test smg-8 for degradation of the endogenous, NMD target generated by unc-54(r293), which lacks a normal polyadenylation site. We probe the effect of smg-8 on the exogenous NMD target produced by myo-3::GFP, which carries a long 3' untranslated region that destabilizes mRNAs. None of these known NMD targets is influenced by smg-8 mutations. In addition, smg-8 animals lack classical Smg mutant phenotypes such as a reduced brood size or abnormal vulva. We conclude that smg-8 is unlikely to encode a component critical for NMD.

Published
2012
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2. Genetic characterization of smg-8 mutants reveals no role in C. elegans nonsense mediated decay

Author

Susan E. Mango and Jacqueline Rosains

Subjects
Untranslated region, Ribosomal Proteins, Nonsense-mediated decay, Mutant, lcsh:Medicine, medicine.disease_cause, Biochemistry, 03 medical and health sciences, 0302 clinical medicine, Model Organisms, Molecular cell biology, stomatognathic system, RNA interference, Genes, Reporter, Gene Order, medicine, Genetics, Animals, RNA, Messenger, Caenorhabditis elegans, Caenorhabditis elegans Proteins, lcsh:Science, Gene, Biology, 030304 developmental biology, 0303 health sciences, Mutation, Multidisciplinary, biology, Alternative splicing, lcsh:R, Animal Models, RNA stability, biology.organism_classification, Nonsense Mediated mRNA Decay, Nucleic acids, Phenotype, RNA processing, Gene Expression Regulation, RNA, lcsh:Q, Gene expression, Gene Function, Animal Genetics, 030217 neurology & neurosurgery, Research Article
Abstract

The nonsense mediated decay (NMD) pathway degrades mRNAs bearing premature translation termination codons. In mammals, SMG-8 has been implicated in the NMD pathway, in part by its association with SMG-1 kinase. Here we use four independent assays to show that C. elegans smg-8 is not required to degrade nonsense-containing mRNAs. We examine the genetic requirement for smg-8 to destabilize the endogenous, natural NMD targets produced by alternative splicing of rpl-7a and rpl-12. We test smg-8 for degradation of the endogenous, NMD target generated by unc-54(r293), which lacks a normal polyadenylation site. We probe the effect of smg-8 on the exogenous NMD target produced by myo-3::GFP, which carries a long 3′ untranslated region that destabilizes mRNAs. None of these known NMD targets is influenced by smg-8 mutations. In addition, smg-8 animals lack classical Smg mutant phenotypes such as a reduced brood size or abnormal vulva. We conclude that smg-8 is unlikely to encode a component critical for NMD.

Published
2012

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