1. Using NextRAD sequencing to infer movement of herbivores among host plants
- Author
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Zhen Fu, Qi Zheng, Brendan Epstein, Joanna L. Kelley, Carmen I. Castillo Carrillo, Alexander V. Karasev, Jennifer Dahan, Andrew S. Jensen, Alan O. Bergland, William E. Snyder, and Doucet, Daniel
- Subjects
0106 biological sciences ,0301 basic medicine ,Plant genetics ,lcsh:Medicine ,Plant Science ,Insect ,Disease Vectors ,Plant Genetics ,01 natural sciences ,Geographical locations ,Vegetables ,Medicine and Health Sciences ,lcsh:Science ,Phylogeny ,media_common ,Genetics ,Multidisciplinary ,Ecology ,biology ,High-Throughput Nucleotide Sequencing ,food and beverages ,Plants ,Trophic Interactions ,Infectious Diseases ,Community Ecology ,Host-Pathogen Interactions ,Infection ,Potato ,Research Article ,Washington ,Bactericera cockerelli ,General Science & Technology ,media_common.quotation_subject ,Solanum ,010603 evolutionary biology ,Zebra chip ,Host-Parasite Interactions ,Hemiptera ,03 medical and health sciences ,Plant-Animal Interactions ,Animals ,Herbivory ,Plant Diseases ,Solanum tuberosum ,Evolutionary Biology ,Herbivore ,Population Biology ,Plant Ecology ,Ecology and Environmental Sciences ,fungi ,lcsh:R ,Organisms ,Computational Biology ,Biology and Life Sciences ,biology.organism_classification ,United States ,Insect Vectors ,Species Interactions ,030104 developmental biology ,Evolutionary biology ,Vector (epidemiology) ,North America ,Biological dispersal ,lcsh:Q ,People and places ,Population Genetics - Abstract
Herbivores often move among spatially interspersed host plants, tracking high-quality resources through space and time. This dispersal is of particular interest for vectors of plant pathogens. Existing molecular tools to track such movement have yielded important insights, but often provide insufficient genetic resolution to infer spread at finer spatiotemporal scales. Here, we explore the use of Nextera-tagmented reductively-amplified DNA (NextRAD) sequencing to infer movement of a highly-mobile winged insect, the potato psyllid (Bactericera cockerelli), among host plants. The psyllid vectors the pathogen that causes zebra chip disease in potato (Solanum tuberosum), but understanding and managing the spread of this pathogen is limited by uncertainty about the insect's host plant(s) outside of the growing season. We identified 1,978 polymorphic loci among psyllids separated spatiotemporally on potato or in patches of bittersweet nightshade (S. dulcumara), a weedy plant proposed to be the source of potato-colonizing psyllids. A subset of the psyllids on potato exhibited genetic similarity to insects on nightshade, consistent with regular movement between these two host plants. However, a second subset of potato-collected psyllids was genetically distinct from those collected on bittersweet nightshade; this suggests that a currently unrecognized source, i.e., other nightshade patches or a third host-plant species, could be contributing to psyllid populations in potato. Oftentimes, dispersal of vectors of pathogens must be tracked at a fine scale in order to understand, predict, and manage disease spread. We demonstrate that emerging sequencing technologies that detect genome-wide SNPs of a vector can be used to infer such localized movement.
- Published
- 2017