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Your search keyword '"Juliana de Freitas Germano"' showing total 2 results
Start Over Author "Juliana de Freitas Germano" Publisher public library of science (plos)
2 results on '"Juliana de Freitas Germano"'

1. Dysregulation of microRNAs and target genes networks in human abdominal aortic aneurysm tissues.

Author

Neire Niara Ferreira de Araujo, Hui Tzu Lin-Wang, Juliana de Freitas Germano, Pedro Silvio Farsky, Andre Feldman, Fabio Henrique Rossi, Nilo Mitsuru Izukawa, Maria de Lourdes Higuchi, Felicio Savioli Neto, Mario Hiroyuki Hirata, and Marcelo Chiara Bertolami

Subjects
Medicine, Science
Abstract

BackgroundAbdominal aortic aneurysm (AAA) is a pathological enlargement of infrarenal aorta close to the aortic bifurcation, and it is an important cause of mortality in the elderly. Therefore, the biomarker identification for early diagnosis is of great interest for clinical benefit. It is known that microRNAs (miRNAs) have important roles via target genes regulation in many diseases. This study aimed to identify miRNAs and their target genes involved in the pathogenesis of AAA.MethodsTissue samples were obtained from patients who underwent AAA surgery and from organ donors (control group). Quantitative PCR Array was applied to assess 84 genes and 384 miRNAs aiming to identify differentially expressed targets (AAA n = 6, control n = 6), followed by validation in a new cohort (AAA n = 18, control n = 6) by regular qPCR. The functional interaction between validated miRNAs and target genes was performed by the Ingenuity Pathway Analysis (IPA) software.ResultsThe screening cohort assessed by PCR array identified 10 genes and 59 miRNAs differentially expressed (≥2-fold change, pConclusionPaired interactions of differentially expressed ALOX5, PTGIS, CX3CL1 genes, and miR-193b-3p, 125b-5p, 150-5p revealed a potentially significant role of the eicosanoid synthesis and metalloproteinase/TIMP pathways in the AAA pathogenesis.

Published
2019
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2. Dysregulation of microRNAs and target genes networks in human abdominal aortic aneurysm tissues

Author

Fabio Henrique Rossi, Nilo Mitsuru Izukawa, Marcelo Chiara Bertolami, Felicio Savioli Neto, Maria de Lourdes Higuchi, Juliana de Freitas Germano, Hui Tzu Lin-Wang, Mario Hiroyuki Hirata, Andre Feldman, Pedro Silvio Farsky, and Neire Niara Ferreira de Araujo

Subjects
Male, 0301 basic medicine, Artificial Gene Amplification and Extension, Pathogenesis, 030204 cardiovascular system & hematology, Pathology and Laboratory Medicine, Bioinformatics, Biochemistry, Polymerase Chain Reaction, Vascular Medicine, Cohort Studies, BIOMARCADORES, Aortic aneurysm, 0302 clinical medicine, Gene expression, Medicine and Health Sciences, Gene Regulatory Networks, Regulation of gene expression, Metalloproteinase, Multidisciplinary, Reverse Transcriptase Polymerase Chain Reaction, Neurochemistry, Middle Aged, Nucleic acids, Real-time polymerase chain reaction, cardiovascular system, Medicine, Female, Neurochemicals, Aneurysms, Signal Transduction, Research Article, Adult, Science, Biology, Research and Analysis Methods, 03 medical and health sciences, microRNA, Genetics, medicine, Humans, Vascular Diseases, Non-coding RNA, Molecular Biology Techniques, Molecular Biology, Gene, Aged, Natural antisense transcripts, Biology and life sciences, Gene Expression Profiling, Proteins, medicine.disease, Gene regulation, Elastin, MicroRNAs, 030104 developmental biology, Gene Expression Regulation, RNA, Eicosanoids, Aortic Aneurysm, Abdominal, Neuroscience
Abstract

BackgroundAbdominal aortic aneurysm (AAA) is a pathological enlargement of infrarenal aorta close to the aortic bifurcation, and it is an important cause of mortality in the elderly. Therefore, the biomarker identification for early diagnosis is of great interest for clinical benefit. It is known that microRNAs (miRNAs) have important roles via target genes regulation in many diseases. This study aimed to identify miRNAs and their target genes involved in the pathogenesis of AAA.MethodsTissue samples were obtained from patients who underwent AAA surgery and from organ donors (control group). Quantitative PCR Array was applied to assess 84 genes and 384 miRNAs aiming to identify differentially expressed targets (AAA n = 6, control n = 6), followed by validation in a new cohort (AAA n = 18, control n = 6) by regular qPCR. The functional interaction between validated miRNAs and target genes was performed by the Ingenuity Pathway Analysis (IPA) software.ResultsThe screening cohort assessed by PCR array identified 10 genes and 59 miRNAs differentially expressed (≥2-fold change, pConclusionPaired interactions of differentially expressed ALOX5, PTGIS, CX3CL1 genes, and miR-193b-3p, 125b-5p, 150-5p revealed a potentially significant role of the eicosanoid synthesis and metalloproteinase/TIMP pathways in the AAA pathogenesis.

Published
2019

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