1. Integrated analysis of microRNA regulation and its interaction with mechanisms of epigenetic regulation in the etiology of systemic lupus erythematosus
- Author
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Lisandro Pacheco Lugo, Lorena Gomez Escorcia, Pablo Navarro Rodriguez, Hernan Lorenzi, Gustavo Aroca Martínez, Cecilia Fernández-Ponce, Joe Luis Villa, Henry J. González Torres, Maria del Carmen Marmolejo, Elkin Navarro Quiroz, Eduardo Sanchez, Kelvin Fernando Navarro, José Villarreal Camacho, Roberto Navarro Quiroz, Augusto Torres, Andres Cadena Bonfanti, and Bioquímica y Biología Molecular, Microbiología, Medicina Preventiva, Salud Pública
- Subjects
0301 basic medicine ,ADN ,Biochemistry ,Epigenesis, Genetic ,White Blood Cells ,0302 clinical medicine ,Animal Cells ,Databases, Genetic ,Medicine and Health Sciences ,Lupus Erythematosus, Systemic ,Gene Regulatory Networks ,Post-Translational Modification ,Regulation of gene expression ,Multidisciplinary ,DNA methylation ,T Cells ,Chromatin ,Nucleic acids ,Histone ,embryonic structures ,Medicine ,Epigenetics ,Post-translational modification ,Cellular Types ,DNA modification ,Chromatin modification ,Research Article ,Chromosome biology ,Cell biology ,In silico ,Immune Cells ,Science ,Immunology ,Computational biology ,Biology ,Systemic Lupus Erythematosus ,Autoimmune Diseases ,03 medical and health sciences ,Systemic lupus erythematosus ,Rheumatology ,microRNA ,DNA-binding proteins ,Genetics ,Transcription factors ,Humans ,Non-coding RNA ,Gene ,Transcription factor ,Genetic Association Studies ,Natural antisense transcripts ,Blood Cells ,Lupus erythematosus ,Biology and life sciences ,Lupus Erythematosus ,Proteins ,DNA ,Regulatory Proteins ,Gene regulation ,body regions ,MicroRNAs ,030104 developmental biology ,Gene Ontology ,Lupus eritematós ,biology.protein ,RNA ,Clinical Immunology ,Gene expression ,Clinical Medicine ,Protein Processing, Post-Translational ,030217 neurology & neurosurgery ,Transcription Factors - Abstract
The aim of this study was to identity in silico the relationships among microRNAs (miRNAs) and genes encoding transcription factors, ubiquitylation, DNA methylation, and histone modifications in systemic lupus erythematosus (SLE). To identify miRNA dysregulation in SLE, we used miR2Disease and PhenomiR for information about miRNAs exhibiting differential regulation in disease and other biological processes, and HMDD for information about experimentally supported human miRNA–disease association data from genetics, epigenetics, circulating miRNAs, and miRNA–target interactions. This information was incorporated into the miRNA analysis. High-throughput sequencing revealed circulating miRNAs associated with kidney damage in patients with SLE. As the main finding of our in silico analysis of miRNAs differentially expressed in SLE and their interactions with disease-susceptibility genes, post-translational modifications, and transcription factors; we highlight 226 miRNAs associated with genes and processes. Moreover, we highlight that alterations of miRNAs such as hsa-miR-30a-5p, hsa-miR-16-5p, hsa-miR-142-5p, and hsa-miR-324-3p are most commonly associated with post-translational modifications. In addition, altered miRNAs that are most frequently associated with susceptibility-related genes are hsa-miR-16-5p, hsamiR- 374a-5p, hsa-miR-34a-5p, hsa-miR-31-5p, and hsa-miR-1-3p.
- Published
- 2019
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