1. Yersinia pestis strains of ancient phylogenetic branch 0.ANT are widely spread in the high-mountain plague foci of Kyrgyzstan
- Author
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Sabyrzhan T. Abdikarimov, Yevgeny G. Oglodin, Yaroslav M. Krasnov, Nikita Yu Nosov, L. M. Kukleva, Aigul K. Dzhaparova, Alexander A. Kuznetsov, N. P. Guseva, Vladimir V. Kutyrev, and G. A. Eroshenko
- Subjects
Bacterial Diseases ,0301 basic medicine ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Pathology and Laboratory Medicine ,Polymerase Chain Reaction ,Medicine and Health Sciences ,Genome Sequencing ,lcsh:Science ,Phylogeny ,Data Management ,Genetics ,Multidisciplinary ,Phylogenetic tree ,biology ,Strain (biology) ,Phylogenetic Analysis ,Yersinia ,Bacterial Pathogens ,Phylogenetics ,Infectious Diseases ,Medical Microbiology ,Pathogens ,Research Article ,SNP array ,DNA, Bacterial ,Computer and Information Sciences ,Yersinia Pestis ,Research and Analysis Methods ,Polymorphism, Single Nucleotide ,Microbiology ,Bubonic plague ,DNA sequencing ,Molecular Genetics ,03 medical and health sciences ,medicine ,Humans ,Evolutionary Systematics ,Kyrgyzstan ,Molecular Biology Techniques ,Sequencing Techniques ,Microbial Pathogens ,Molecular Biology ,Taxonomy ,Whole genome sequencing ,Plague ,Evolutionary Biology ,Bacteria ,lcsh:R ,Organisms ,Biology and Life Sciences ,Paleontology ,medicine.disease ,biology.organism_classification ,Plagues ,030104 developmental biology ,Yersinia pestis ,Earth Sciences ,lcsh:Q ,Paleogenetics - Abstract
Fifty six Yersinia pestis strains, isolated over the period of more than 50 years in three high-mountain foci of Kyrgyzstan (Tien Shan, Alai, and Talas), have been characterized by means of PCR and single nucleotide polymorphism (SNP) typing methods. Seven of these strains were also characterized by means of whole genome sequencing and genome-wide SNP phylogenetic analysis. It was found that forty two strains belong to 0.ANT2, 0.ANT3 and 0.ANT5 phylogenetic branches. From these, strains of 0.ANT2 and 0.ANT3 branches were earlier detected in China only, whereas 0.ANT5 phylogenetic branch was identified for Y. pestis phylogeny for the first time. According to the results of genome-wide SNP analysis, 0.ANT5 strains are ones of the most closely related to Y. pestis strain responsible for the Justinianic Plague. We have also found out that four of the studied strains belong to the phylogenetic branch 2.MED1, and ten strains from Talas high-mountain focus belong to the phylogenetic branch 0.PE4 (sub-branch 0.PE4t). Established diversity of Y. pestis strains and extensive dissemination of the strains pertaining to the 0.ANT branch confirm the antiquity of the mentioned above plague foci and suggest that strains of the 0.ANT branch, which serve as precursors for all highly virulent Y. pestis strains, had their origin in the Tien Shan mountains.
- Published
- 2017
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