4 results on '"Patrizia Barozzi"'
Search Results
2. Identification and validation of diagnostic cut-offs of the ELISpot assay for the diagnosis of invasive aspergillosis in high-risk patients.
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Francesca Bettelli, Daniela Vallerini, Ivana Lagreca, Patrizia Barozzi, Giovanni Riva, Vincenzo Nasillo, Ambra Paolini, Roberto D'Amico, Fabio Forghieri, Monica Morselli, Valeria Pioli, Andrea Gilioli, Davide Giusti, Andrea Messerotti, Paola Bresciani, Angela Cuoghi, Elisabetta Colaci, Roberto Marasca, Livio Pagano, Anna Candoni, Johan Maertens, Pierluigi Viale, Cristina Mussini, Rossella Manfredini, Enrico Tagliafico, Mario Sarti, Tommaso Trenti, Russell Lewis, Patrizia Comoli, Albino Eccher, Mario Luppi, and Leonardo Potenza more...
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Medicine ,Science - Abstract
ObjectiveWe investigated the performance of enzyme linked immunospot (ELISpot) assay for the diagnosis of invasive aspergillosis (IA) in high-risk patients with hematologic malignancies.MethodsWe prospectively enrolled two cohorts of patients undergoing intensive myelosuppressive or immunosuppressive treatments at high risk for IA. ELISpot was performed to detect Aspergillus-specific T cells producing Interleukin-10.ResultsIn the discovery cohort, a derived cut-off of 40 spot forming cells (SFCs)/106 PBMCs has shown to correctly classify IA cases with a sensitivity and specificity of 89.5% and 88.6%, respectively. This cut-off is lowered to 25 SFC when considering the subset of possible IA patients, with sensitivity and specificity of 76% and 93%, respectively. The application of the 40 SFCs cut-off to the validation cohort resulted in a positivity rate of 83.3% in proven/probable cases and a negativity rate of 92.5% in possible/non-IA cases. Adopting the 25 SCFs cut-off, the assay resulted positive in 83.3% of proven/probable cases while it resulted negative in 66.7% of possible/non-IA cases.ConclusionsELISpot shows promises in the diagnosis of IA and the possibility to use two distinct cut-offs with similar diagnostic performances according to patients' different pre-test probability of infection can widen its use in patients at risk. more...
- Published
- 2024
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Catalog
3. Mucorales-Specific T Cells in Patients with Hematologic Malignancies.
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Leonardo Potenza, Daniela Vallerini, Patrizia Barozzi, Giovanni Riva, Andrea Gilioli, Fabio Forghieri, Anna Candoni, Simone Cesaro, Chiara Quadrelli, Johan Maertens, Giulio Rossi, Monica Morselli, Mauro Codeluppi, Cristina Mussini, Elisabetta Colaci, Andrea Messerotti, Ambra Paolini, Monica Maccaferri, Valeria Fantuzzi, Cinzia Del Giovane, Alessandro Stefani, Uliano Morandi, Rossana Maffei, Roberto Marasca, Franco Narni, Renato Fanin, Patrizia Comoli, Luigina Romani, Anne Beauvais, Pier Luigi Viale, Jean Paul Latgè, Russell E Lewis, and Mario Luppi more...
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Medicine ,Science - Abstract
BACKGROUND:Invasive mucormycosis (IM) is an emerging life-threatening fungal infection. It is difficult to obtain a definite diagnosis and to initiate timely intervention. Mucorales-specific T cells occur during the course of IM and are involved in the clearance of the infection. We have evaluated the feasibility of detecting Mucorales-specific T cells in hematological patients at risk for IM, and have correlated the detection of such cells with the clinical conditions of the patients. METHODS AND FINDINGS:By using an enzyme linked immunospot assay, the presence of Mucorales-specific T cells in peripheral blood (PB) samples has been investigated at three time points during high-dose chemotherapy for hematologic malignancies. Mucorales-specific T cells producing interferon-γ, interleukin-10 and interleukin-4 were analysed in order to detect a correlation between the immune response and the clinical picture. Twenty-one (10.3%) of 204 patients, accounting for 32 (5.3%) of 598 PB samples, tested positive for Mucorales-specific T cells. Two groups could be identified. Group 1, including 15 patients without signs or symptoms of invasive fungal diseases (IFD), showed a predominance of Mucorales-specific T cells producing interferon-gamma. Group 2 included 6 patients with a clinical picture consistent with invasive fungal disease (IFD): 2 cases of proven IM and 4 cases of possible IFD. The proven patients had significantly higher number of Mucorales-specific T cells producing interleukin-10 and interleukin-4 and higher rates of positive samples by using derived diagnostic cut-offs when compared with the 15 patients without IFD. CONCLUSIONS:Mucorales-specific T cells can be detected and monitored in patients with hematologic malignancies at risk for IM. Mucorales-specific T cells polarized to the production of T helper type 2 cytokines are associated with proven IM and may be evaluated as a surrogate diagnostic marker for IM. more...
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- 2016
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4. Characterization of specific immune responses to different Aspergillus antigens during the course of invasive Aspergillosis in hematologic patients.
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Leonardo Potenza, Daniela Vallerini, Patrizia Barozzi, Giovanni Riva, Fabio Forghieri, Anne Beauvais, Remi Beau, Anna Candoni, Johan Maertens, Giulio Rossi, Monica Morselli, Eleonora Zanetti, Chiara Quadrelli, Mauro Codeluppi, Giovanni Guaraldi, Livio Pagano, Morena Caira, Cinzia Del Giovane, Monica Maccaferri, Alessandro Stefani, Uliano Morandi, Giovanni Tazzioli, Massimo Girardis, Mario Delia, Giorgina Specchia, Giuseppe Longo, Roberto Marasca, Franco Narni, Francesco Merli, Annalisa Imovilli, Giovanni Apolone, Agostinho Carvalho, Patrizia Comoli, Luigina Romani, Jean Paul Latgè, and Mario Luppi more...
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Medicine ,Science - Abstract
Several studies in mouse model of invasive aspergillosis (IA) and in healthy donors have shown that different Aspergillus antigens may stimulate different adaptive immune responses. However, the occurrence of Aspergillus-specific T cells have not yet been reported in patients with the disease. In patients with IA, we have investigated during the infection: a) whether and how specific T-cell responses to different Aspergillus antigens occur and develop; b) which antigens elicit the highest frequencies of protective immune responses and, c) whether such protective T cells could be expanded ex-vivo. Forty hematologic patients have been studied, including 22 patients with IA and 18 controls. Specific T cells producing IL-10, IFN-γ, IL-4 and IL-17A have been characterized through enzyme linked immunospot and cytokine secretion assays on 88 peripheral blood (PB) samples, by using the following recombinant antigens: GEL1p, CRF1p, PEP1p, SOD1p, α1-3glucan, β1-3glucan, galactomannan. Specific T cells were expanded through short term culture. Aspergillus-specific T cells producing non-protective interleukin-10 (IL-10) and protective interferon-gamma (IFN-γ) have been detected to all the antigens only in IA patients. Lower numbers of specific T cells producing IL-4 and IL-17A have also been shown. Protective T cells targeted predominantly Aspergillus cell wall antigens, tended to increase during the IA course and to be associated with a better clinical outcome. Aspergillus-specific T cells could be successfully generated from the PB of 8 out of 8 patients with IA and included cytotoxic subsets able to lyse Aspergillus hyphae. Aspergillus specific T-cell responses contribute to the clearance of the pathogen in immunosuppressed patients with IA and Aspergillus cell wall antigens are those mainly targeted by protective immune responses. Cytotoxic specific T cells can be expanded from immunosuppressed patients even during the infection by using the above mentioned antigens. These findings may be exploited for immunotherapeutic purposes in patients with IA. more...
- Published
- 2013
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