9 results on '"Yajun He"'
Search Results
2. Noncanonical TGF-β signaling leads to FBXO3-mediated degradation of ΔNp63α promoting breast cancer metastasis and poor clinical prognosis.
- Author
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Mengmeng Niu, Yajun He, Jing Xu, Liangping Ding, Tao He, Yong Yi, Mengyuan Fu, Rongtian Guo, Fengtian Li, Hu Chen, Ye-Guang Chen, and Zhi-Xiong Jim Xiao
- Subjects
Biology (General) ,QH301-705.5 - Abstract
Transforming growth factor-β (TGF-β) signaling plays a critical role in promoting epithelial-to-mesenchymal transition (EMT), cell migration, invasion, and tumor metastasis. ΔNp63α, the major isoform of p63 protein expressed in epithelial cells, is a key transcriptional regulator of cell adhesion program and functions as a critical metastasis suppressor. It has been documented that the expression of ΔNp63α is tightly controlled by oncogenic signaling and is frequently reduced in advanced cancers. However, whether TGF-β signaling regulates ΔNp63α expression in promoting metastasis is largely unclear. In this study, we demonstrate that activation of TGF-β signaling leads to stabilization of E3 ubiquitin ligase FBXO3, which, in turn, targets ΔNp63α for proteasomal degradation in a Smad-independent but Erk-dependent manner. Knockdown of FBXO3 or restoration of ΔNp63α expression effectively rescues TGF-β-induced EMT, cell motility, and tumor metastasis in vitro and in vivo. Furthermore, clinical analyses reveal a significant correlation among TGF-β receptor I (TβRI), FBXO3, and p63 protein expression and that high expression of TβRI/FBXO3 and low expression of p63 are associated with poor recurrence-free survival (RFS). Together, these results demonstrate that FBXO3 facilitates ΔNp63α degradation to empower TGF-β signaling in promoting tumor metastasis and that the TβRI-FBXO3-ΔNp63α axis is critically important in breast cancer development and clinical prognosis. This study suggests that FBXO3 may be a potential therapeutic target for advanced breast cancer treatment.
- Published
- 2021
- Full Text
- View/download PDF
3. Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors under Multiple Stresses in Brassica napus.
- Author
-
Yajun He, Shaoshuai Mao, Yulong Gao, Liying Zhu, Daoming Wu, Yixin Cui, Jiana Li, and Wei Qian
- Subjects
Medicine ,Science - Abstract
WRKY transcription factors play important roles in responses to environmental stress stimuli. Using a genome-wide domain analysis, we identified 287 WRKY genes with 343 WRKY domains in the sequenced genome of Brassica napus, 139 in the A sub-genome and 148 in the C sub-genome. These genes were classified into eight groups based on phylogenetic analysis. In the 343 WRKY domains, a total of 26 members showed divergence in the WRKY domain, and 21 belonged to group I. This finding suggested that WRKY genes in group I are more active and variable compared with genes in other groups. Using genome-wide identification and analysis of the WRKY gene family in Brassica napus, we observed genome duplication, chromosomal/segmental duplications and tandem duplication. All of these duplications contributed to the expansion of the WRKY gene family. The duplicate segments that were detected indicated that genome duplication events occurred in the two diploid progenitors B. rapa and B. olearecea before they combined to form B. napus. Analysis of the public microarray database and EST database for B. napus indicated that 74 WRKY genes were induced or preferentially expressed under stress conditions. According to the public QTL data, we identified 77 WRKY genes in 31 QTL regions related to various stress tolerance. We further evaluated the expression of 26 BnaWRKY genes under multiple stresses by qRT-PCR. Most of the genes were induced by low temperature, salinity and drought stress, indicating that the WRKYs play important roles in B. napus stress responses. Further, three BnaWRKY genes were strongly responsive to the three multiple stresses simultaneously, which suggests that these 3 WRKY may have multi-functional roles in stress tolerance and can potentially be used in breeding new rapeseed cultivars. We also found six tandem repeat pairs exhibiting similar expression profiles under the various stress conditions, and three pairs were mapped in the stress related QTL regions, indicating tandem duplicate WRKYs in the adaptive responses to environmental stimuli during the evolution process. Our results provide a framework for future studies regarding the function of WRKY genes in response to stress in B. napus.
- Published
- 2016
- Full Text
- View/download PDF
4. E. adenophorum Induces Cell Cycle and Apoptosis of Renal Cells through Mitochondrial Pathway and Caspase Activation in Saanen Goat.
- Author
-
Yajun He, Weihong Chen, Yanchun Hu, Biao Luo, Lei Wu, Yan Qiao, Quan Mo, Ruiguang Xu, Yancheng Zhou, Zhihua Ren, Zhicai Zuo, Junliang Deng, Guangneng Peng, Wei He, and Yahui Wei
- Subjects
Medicine ,Science - Abstract
The cytotoxicity effects of E. adenophorum on cell cycle and apoptosis of renal cells in Saanen goat was evaluated by TUNEL, DAPI, AO/EB staining, DNA fragmentation assay, Caspase activity, Western-blot, qRT-PCR and flow cytometry analysis. 16 saanen goats randomly divided into four groups were fed on 0%, 40%, 60% and 80% E. adenophorum diets. The Results showed that E. adenophorum induced typical apoptotic features of renal cells. E. adenophorum significantly suppressed renal cells viability, caused cell cycle activity arrest and induced typical apoptotic features in a dose-dependent manner. However, the protein levels of Fas/FasL, Bid and caspase-8 did not appear significant changes in the process of E. adenophorum-induced apoptosis. Moreover, E. adenophorum administration slightly decreased Bcl-2 expression, promoted Bax translocation to mitochondria, triggered the release of Cyt c from mitochondria into cytosol and activated caspase-9, -3, and cleaved PARP. The mitochondrial p53 translocation was significantly activated, accompanied by a significant increase in the loss of ΔΨm, Cyt c release and caspase-9 activation. Above all, these data suggest that E. adenophorum induces renal cells apoptosis via the activation of mitochondria-mediated apoptosis pathway in renal cells. These findings may provide new insights to understand the mechanisms involved in E. adenophorum-caused cytotoxicity of renal cells.
- Published
- 2015
- Full Text
- View/download PDF
5. A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L.
- Author
-
Liezhao Liu, Cunmin Qu, Benjamin Wittkop, Bin Yi, Yang Xiao, Yajun He, Rod J Snowdon, and Jiana Li
- Subjects
Medicine ,Science - Abstract
A high density genetic linkage map for the complex allotetraploid crop species Brassica napus (oilseed rape) was constructed in a late-generation recombinant inbred line (RIL) population, using genome-wide single nucleotide polymorphism (SNP) markers assayed by the Brassica 60 K Infinium BeadChip Array. The linkage map contains 9164 SNP markers covering 1832.9 cM. 1232 bins account for 7648 of the markers. A subset of 2795 SNP markers, with an average distance of 0.66 cM between adjacent markers, was applied for QTL mapping of seed colour and the cell wall fiber components acid detergent lignin (ADL), cellulose and hemicellulose. After phenotypic analyses across four different environments a total of 11 QTL were detected for seed colour and fiber traits. The high-density map considerably improved QTL resolution compared to the previous low-density maps. A previously identified major QTL with very high effects on seed colour and ADL was pinpointed to a narrow genome interval on chromosome A09, while a minor QTL explaining 8.1% to 14.1% of variation for ADL was detected on chromosome C05. Five and three QTL accounting for 4.7% to 21.9% and 7.3% to 16.9% of the phenotypic variation for cellulose and hemicellulose, respectively, were also detected. To our knowledge this is the first description of QTL for seed cellulose and hemicellulose in B. napus, representing interesting new targets for improving oil content. The high density SNP genetic map enables navigation from interesting B. napus QTL to Brassica genome sequences, giving useful new information for understanding the genetics of key seed quality traits in rapeseed.
- Published
- 2013
- Full Text
- View/download PDF
6. Noncanonical TGF-β signaling leads to FBXO3-mediated degradation of ΔNp63α promoting breast cancer metastasis and poor clinical prognosis
- Author
-
Liangping Ding, Yajun He, Ye-Guang Chen, Rongtian Guo, Mengyuan Fu, Zhi-Xiong Jim Xiao, Fengtian Li, Yong Yi, Tao He, Mengmeng Niu, Hu Chen, and Jing Xu
- Subjects
RNA viruses ,0301 basic medicine ,Cell signaling ,Protein Expression ,Signal transduction ,Pathology and Laboratory Medicine ,Metastasis ,Medical Conditions ,0302 clinical medicine ,Transforming Growth Factor beta ,Basic Cancer Research ,Breast Tumors ,Medicine and Health Sciences ,HaCaT Cells ,Protein Isoforms ,Biology (General) ,Neoplasm Metastasis ,General Neuroscience ,Signaling cascades ,Cell migration ,Ubiquitin ligase ,Infectious Diseases ,Oncology ,Medical Microbiology ,Viral Pathogens ,030220 oncology & carcinogenesis ,Viruses ,Female ,Pathogens ,General Agricultural and Biological Sciences ,Research Article ,Cell biology ,Epithelial-Mesenchymal Transition ,Infectious Disease Control ,QH301-705.5 ,Ubiquitin-Protein Ligases ,SMAD signaling ,Breast Neoplasms ,Biology ,Research and Analysis Methods ,Microbiology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Cell Line, Tumor ,Breast Cancer ,Retroviruses ,Gene Expression and Vector Techniques ,medicine ,Humans ,Metastasis suppressor ,Epithelial–mesenchymal transition ,Molecular Biology Techniques ,Cell adhesion ,Microbial Pathogens ,Molecular Biology ,Molecular Biology Assays and Analysis Techniques ,Biology and life sciences ,General Immunology and Microbiology ,Tumor Suppressor Proteins ,Lentivirus ,Organisms ,Cancers and Neoplasms ,medicine.disease ,Vector-Borne Diseases ,HEK293 Cells ,030104 developmental biology ,TGF-beta signaling cascade ,biology.protein ,Cancer research ,Transforming growth factor - Abstract
Transforming growth factor-β (TGF-β) signaling plays a critical role in promoting epithelial-to-mesenchymal transition (EMT), cell migration, invasion, and tumor metastasis. ΔNp63α, the major isoform of p63 protein expressed in epithelial cells, is a key transcriptional regulator of cell adhesion program and functions as a critical metastasis suppressor. It has been documented that the expression of ΔNp63α is tightly controlled by oncogenic signaling and is frequently reduced in advanced cancers. However, whether TGF-β signaling regulates ΔNp63α expression in promoting metastasis is largely unclear. In this study, we demonstrate that activation of TGF-β signaling leads to stabilization of E3 ubiquitin ligase FBXO3, which, in turn, targets ΔNp63α for proteasomal degradation in a Smad-independent but Erk-dependent manner. Knockdown of FBXO3 or restoration of ΔNp63α expression effectively rescues TGF-β-induced EMT, cell motility, and tumor metastasis in vitro and in vivo. Furthermore, clinical analyses reveal a significant correlation among TGF-β receptor I (TβRI), FBXO3, and p63 protein expression and that high expression of TβRI/FBXO3 and low expression of p63 are associated with poor recurrence-free survival (RFS). Together, these results demonstrate that FBXO3 facilitates ΔNp63α degradation to empower TGF-β signaling in promoting tumor metastasis and that the TβRI-FBXO3-ΔNp63α axis is critically important in breast cancer development and clinical prognosis. This study suggests that FBXO3 may be a potential therapeutic target for advanced breast cancer treatment., TGF-β signaling is critical for promoting cancer metastasis, primarily via Smad-dependent regulation of the epithelial-mesenchymal transition; this study reveals that non-canonical TGF-β signaling stabilizes the E3 ubiquitin ligase FBXO3 to target ΔNp63α for degradation, resulting in downregulation of adhesion molecules and promotion of breast cancer metastasis.
- Published
- 2021
7. Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors under Multiple Stresses in Brassica napus
- Author
-
Yixin Cui, Jiana Li, Wei Qian, Yajun He, Liying Zhu, Shaoshuai Mao, Yulong Gao, and Daoming Wu
- Subjects
0106 biological sciences ,0301 basic medicine ,Amino Acid Motifs ,Arabidopsis ,Gene Expression ,lcsh:Medicine ,Plant Science ,Plant Genetics ,01 natural sciences ,Genome ,Database and Informatics Methods ,Gene Expression Regulation, Plant ,Plant Resistance to Abiotic Stress ,Gene Duplication ,Gene duplication ,Databases, Genetic ,Plant Genomics ,lcsh:Science ,Conserved Sequence ,Phylogeny ,Segmental duplication ,Plant Proteins ,Genetics ,Multidisciplinary ,Ecology ,Chromosome Biology ,Physics ,Classical Mechanics ,food and beverages ,Genomics ,Genomic Databases ,Adaptation, Physiological ,Tandem Repeats ,Plant Physiology ,Multigene Family ,Physical Sciences ,Mechanical Stress ,Tandem exon duplication ,Sequence Analysis ,Genome, Plant ,Research Article ,Biotechnology ,Quantitative Trait Loci ,Biology ,Quantitative trait locus ,Research and Analysis Methods ,Genes, Plant ,Chromosomes ,Chromosomes, Plant ,03 medical and health sciences ,Protein Domains ,Sequence Motif Analysis ,Stress, Physiological ,Plant-Environment Interactions ,Gene family ,Plant Defenses ,Computer Simulation ,Repeated Sequences ,Amino Acid Sequence ,Molecular Biology Techniques ,Sequencing Techniques ,Gene ,Molecular Biology ,Plant Ecology ,Ecology and Environmental Sciences ,Brassica napus ,lcsh:R ,Biology and Life Sciences ,Computational Biology ,Cell Biology ,Plant Pathology ,Genome Analysis ,WRKY protein domain ,030104 developmental biology ,Thermal Stresses ,Biological Databases ,Plant Biotechnology ,lcsh:Q ,010606 plant biology & botany ,Transcription Factors - Abstract
WRKY transcription factors play important roles in responses to environmental stress stimuli. Using a genome-wide domain analysis, we identified 287 WRKY genes with 343 WRKY domains in the sequenced genome of Brassica napus, 139 in the A sub-genome and 148 in the C sub-genome. These genes were classified into eight groups based on phylogenetic analysis. In the 343 WRKY domains, a total of 26 members showed divergence in the WRKY domain, and 21 belonged to group I. This finding suggested that WRKY genes in group I are more active and variable compared with genes in other groups. Using genome-wide identification and analysis of the WRKY gene family in Brassica napus, we observed genome duplication, chromosomal/segmental duplications and tandem duplication. All of these duplications contributed to the expansion of the WRKY gene family. The duplicate segments that were detected indicated that genome duplication events occurred in the two diploid progenitors B. rapa and B. olearecea before they combined to form B. napus. Analysis of the public microarray database and EST database for B. napus indicated that 74 WRKY genes were induced or preferentially expressed under stress conditions. According to the public QTL data, we identified 77 WRKY genes in 31 QTL regions related to various stress tolerance. We further evaluated the expression of 26 BnaWRKY genes under multiple stresses by qRT-PCR. Most of the genes were induced by low temperature, salinity and drought stress, indicating that the WRKYs play important roles in B. napus stress responses. Further, three BnaWRKY genes were strongly responsive to the three multiple stresses simultaneously, which suggests that these 3 WRKY may have multi-functional roles in stress tolerance and can potentially be used in breeding new rapeseed cultivars. We also found six tandem repeat pairs exhibiting similar expression profiles under the various stress conditions, and three pairs were mapped in the stress related QTL regions, indicating tandem duplicate WRKYs in the adaptive responses to environmental stimuli during the evolution process. Our results provide a framework for future studies regarding the function of WRKY genes in response to stress in B. napus.
- Published
- 2016
8. A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L
- Author
-
Xiao Yang, Yajun He, Liezhao Liu, Jiana Li, Cunmin Qu, Rod J. Snowdon, Bin Yi, and Benjamin Wittkop
- Subjects
Rapeseed ,Agricultural Biotechnology ,Science ,Quantitative Trait Loci ,Population ,Brassica ,Color ,Single-nucleotide polymorphism ,Plant Science ,Quantitative trait locus ,Plant Genetics ,Polymorphism, Single Nucleotide ,Gene mapping ,Cell Wall ,Genome Analysis Tools ,Genetic linkage ,Botany ,Genetics ,Plant Genomics ,SNP ,Trait Locus Analysis ,education ,Biology ,education.field_of_study ,Multidisciplinary ,biology ,Brassica napus ,Chromosome Mapping ,food and beverages ,Agriculture ,Genetic Maps ,Genomics ,biology.organism_classification ,Seeds ,Genetic Polymorphism ,Medicine ,Plant Biotechnology ,Population Genetics ,Research Article - Abstract
A high density genetic linkage map for the complex allotetraploid crop species Brassica napus (oilseed rape) was constructed in a late-generation recombinant inbred line (RIL) population, using genome-wide single nucleotide polymorphism (SNP) markers assayed by the Brassica 60 K Infinium BeadChip Array. The linkage map contains 9164 SNP markers covering 1832.9 cM. 1232 bins account for 7648 of the markers. A subset of 2795 SNP markers, with an average distance of 0.66 cM between adjacent markers, was applied for QTL mapping of seed colour and the cell wall fiber components acid detergent lignin (ADL), cellulose and hemicellulose. After phenotypic analyses across four different environments a total of 11 QTL were detected for seed colour and fiber traits. The high-density map considerably improved QTL resolution compared to the previous low-density maps. A previously identified major QTL with very high effects on seed colour and ADL was pinpointed to a narrow genome interval on chromosome A09, while a minor QTL explaining 8.1% to 14.1% of variation for ADL was detected on chromosome C05. Five and three QTL accounting for 4.7% to 21.9% and 7.3% to 16.9% of the phenotypic variation for cellulose and hemicellulose, respectively, were also detected. To our knowledge this is the first description of QTL for seed cellulose and hemicellulose in B. napus, representing interesting new targets for improving oil content. The high density SNP genetic map enables navigation from interesting B. napus QTL to Brassica genome sequences, giving useful new information for understanding the genetics of key seed quality traits in rapeseed.
- Published
- 2013
9. E. adenophorum Induces Cell Cycle and Apoptosis of Renal Cells through Mitochondrial Pathway and Caspase Activation in Saanen Goat
- Author
-
Ruiguang Xu, Wei He, Quan Mo, Yan Qiao, Lei Wu, Zhihua Ren, Yanchun Hu, Yajun He, Junliang Deng, Biao Luo, Zhicai Zuo, Yahui Wei, Weihong Chen, Yancheng Zhou, and Guangneng Peng
- Subjects
lcsh:Medicine ,Apoptosis ,Mitochondrion ,Kidney ,Fas ligand ,chemistry.chemical_compound ,Animals ,Ageratina ,DAPI ,lcsh:Science ,bcl-2-Associated X Protein ,Multidisciplinary ,TUNEL assay ,biology ,Goats ,Cytochrome c ,lcsh:R ,Cell Cycle ,Cell Cycle Checkpoints ,Cell cycle ,Molecular biology ,Mitochondria ,Cell biology ,Proto-Oncogene Proteins c-bcl-2 ,chemistry ,Caspases ,biology.protein ,DNA fragmentation ,lcsh:Q ,Research Article - Abstract
The cytotoxicity effects of E. adenophorum on cell cycle and apoptosis of renal cells in Saanen goat was evaluated by TUNEL, DAPI, AO/EB staining, DNA fragmentation assay, Caspase activity, Western-blot, qRT-PCR and flow cytometry analysis. 16 saanen goats randomly divided into four groups were fed on 0%, 40%, 60% and 80% E. adenophorum diets. The Results showed that E. adenophorum induced typical apoptotic features of renal cells. E. adenophorum significantly suppressed renal cells viability, caused cell cycle activity arrest and induced typical apoptotic features in a dose-dependent manner. However, the protein levels of Fas/FasL, Bid and caspase-8 did not appear significant changes in the process of E. adenophorum-induced apoptosis. Moreover, E. adenophorum administration slightly decreased Bcl-2 expression, promoted Bax translocation to mitochondria, triggered the release of Cyt c from mitochondria into cytosol and activated caspase-9, -3, and cleaved PARP. The mitochondrial p53 translocation was significantly activated, accompanied by a significant increase in the loss of ΔΨm, Cyt c release and caspase-9 activation. Above all, these data suggest that E. adenophorum induces renal cells apoptosis via the activation of mitochondria-mediated apoptosis pathway in renal cells. These findings may provide new insights to understand the mechanisms involved in E. adenophorum-caused cytotoxicity of renal cells.
- Published
- 2015
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