1. Intracellular transport of inositol-containing sphingolipids in the yeast, Saccharomyces cerevisiae
- Author
-
Günther Daum and P. Hechtberger
- Subjects
Biophysics ,Golgi Apparatus ,Cyclopentanes ,Saccharomyces cerevisiae ,Biology ,Cycloheximide ,Ceramides ,Endoplasmic Reticulum ,Biochemistry ,Sphingolipid ,Cell membrane ,chemistry.chemical_compound ,symbols.namesake ,Structural Biology ,parasitic diseases ,Genetics ,medicine ,cardiovascular diseases ,Molecular Biology ,Brefeldin A ,Nocodazole ,Endoplasmic reticulum ,Cell Membrane ,Biological Transport ,Cell Biology ,Golgi apparatus ,Membrane transport ,Lipid transport ,Cell biology ,Secretory protein ,medicine.anatomical_structure ,chemistry ,symbols ,lipids (amino acids, peptides, and proteins) ,Protein secretion ,Inositol ,Plasma membrane - Abstract
Organelles of the early protein secretion pathway (ER, Golgi) are involved in biosynthesis and intracellular migration of the yeast sphingolipids, inositolphosphorylceramide (IPC), mannosylinositolphosphorylceramide (MIPC), and mannosyldiinositolphosphorylceramide (M(IP)2C). Cycloheximide and nocodazole neither block biosynthesis of sphingolipids, nor ER to Golgi transport of IPC. In contrast, treatment of yeast cells with brefeldin A, which affects integrity of the Golgi, decreases formation of IPC and MIPC. Interruption of late steps of protein secretion (Golgi to plasma membrane transport) in temperature-sensitive secretory mutants prevents sphingolipids from being transported to the cell periphery.
- Full Text
- View/download PDF